A5075619750- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Atherosclerosis and Cardiovascular Diseases
- Algal biology and biofuel production
- Forensic Toxicology and Drug Analysis
- Semiconductor materials and interfaces
- Glycogen Storage Diseases and Myoclonus
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Surface and Thin Film Phenomena
- Barrier Structure and Function Studies
- Steroid Chemistry and Biochemistry
- Cholesterol and Lipid Metabolism
- Pesticide Residue Analysis and Safety
- Antibiotics Pharmacokinetics and Efficacy
- Pharmacogenetics and Drug Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Sphingolipid Metabolism and Signaling
- Carcinogens and Genotoxicity Assessment
- Biocrusts and Microbial Ecology
- Advancements in Semiconductor Devices and Circuit Design
- Enzyme Structure and Function
Fraunhofer Institute for Translational Medicine and Pharmacology
2021
University of Tartu
2017-2020
St George's, University of London
2018-2020
University Hospital Leipzig
2013-2017
A rapid liquid chromatography–tandem mass spectrometric (LC–MS/MS) method was developed and validated for the quantification of reactive oxygen species (ROS) derived free oxysterols cholesterol in human plasma atherosclerotic plaque. In vitro autoxidation during sample pretreatment avoided by applying only one protein precipitation re-concentration step using 80 μl plasma. For preparation 10 mg plaques an additional liquid–liquid extraction included. Free 7-keto-, 7-α/ß-hydroxy-,...
The precise regulation of blood-brain barrier (BBB) permeability for immune cells and blood-borne substances is essential to maintain brain homeostasis. Sphingosine-1-phosphate (S1P), a lipid signaling molecule enriched in plasma, known affect BBB permeability. Previous studies focused on endothelial S1P receptors 1 2, reporting barrier-protective effect S1P1 barrier-disruptive S1P2. Here, we present novel data characterizing the expression, localization, function receptor 4 (S1P4) primary...
3,4-Methylenedioxymethamphetamine (MDMA, Ecstasy) tablets are widely used recreationally, and not only vary in appearance, but also MDMA content. Recently, the prevalence of high-content is concern to public health authorities. To compare UK data with other countries, we evaluated content 412 collected from UK, 2001-2018, investigated within-batch variability for a sub-set these samples. In addition, dissolution profiles using pharmaceutical industry-standard experiments on 247 tablets. All...
Traditionally, bioanalytical laboratories do not report actual concentrations for samples with results below the LOQ (BLQ) in pharmacokinetic studies. BLQ values are outside method calibration range established during validation and no data available to support reliability of these values. However, ignoring can contribute bias imprecision model-based analyses. From this perspective, routine use would be advantageous. We like initiate an interdisciplinary debate on important topic by...
Abstract Laborious sample pretreatment of biological samples represents the most limiting factor for translation targeted proteomics assays from research to clinical routine. An optimized method simultaneous quantitation 12 major apolipoproteins (apos) combining on‐line SPE and fast LC‐MS/MS analysis in 6.5 min total run time was developed, reducing manual 3 μL serum or plasma by 60%. Within‐run between‐day imprecisions below 10 15% ( n = 10) high recovery rates (94–131%) were obtained...
Sample preparation for the analysis of clinical samples with mass spectrometer (MS) can be extensive and expensive. Simplifying speeding up process would very beneficial. This paper reports sponge spray-a novel sampling direct MS approach-attempting exactly that. It enables without any sample from dried blood, plasma, urine. The tip a volumetric absorptive microsampling device is used to collect an exact amount that same electrospray directed into spectrometer. We demonstrate here that,...
Albeit achieving the X-ray diffraction structure of dimeric photosystem II core complexes (dPSIIcc) at atomic resolution, nature detergent belt surrounding dPSIIcc remains ambiguous. Therefore, solution whole detergent-protein complex Thermosynechococcus elongatus (T. elongatus) solubilized in n-dodecyl-ß-d-maltoside (ßDM) was investigated by a combination small-angle scattering (SAXS) and neutron (SANS) with contrast variation. First, studied separately SANS experiments using 5% D2O....
This research focuses on retention mechanisms in a LC column with C18 stationary phase when novel eluent additives (HFIP, HFTB and TFE as well NFTB perfluoropinacol) are used. The factors between conventional ones like ammonium acetate bicarbonate at different pH values were compared. A simple set of drug-like molecules, widely spread over logP values, containing protonated deprotonated acids bases was selected for this investigation. HFIP, HFTB, PP demonstrated strong influence basic polar...
Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is an ultra-rare disorder caused by mutations in TYMP, leading to a deficiency thymidine phosphorylase and subsequent systemic accumulation of 2’-deoxyuridine. Erythrocyte-encapsulated (EE-TP) under clinical development as enzyme replacement therapy for MNGIE. Bioanalytical methods were developed according regulatory guidelines the quantification 2’-deoxyuridine plasma urine using liquid chromatography-tandem mass spectrometry...