A5000783572- Photosynthetic Processes and Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- Light effects on plants
- Antioxidant Activity and Oxidative Stress
- Algal biology and biofuel production
- Plant Stress Responses and Tolerance
- Electrochemical sensors and biosensors
- Marine and coastal ecosystems
- Photochemistry and Electron Transfer Studies
- Lipid metabolism and biosynthesis
- Mitochondrial Function and Pathology
- Quantum Dots Synthesis And Properties
- Porphyrin and Phthalocyanine Chemistry
- Plant Gene Expression Analysis
- Plant responses to elevated CO2
- Water Quality Monitoring and Analysis
- Origins and Evolution of Life
- Plant biochemistry and biosynthesis
- Gold and Silver Nanoparticles Synthesis and Applications
- Microbial Fuel Cells and Bioremediation
- Electrochemical Analysis and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Molecular Junctions and Nanostructures
- Molecular spectroscopy and chirality
Charles University
2016-2024
University of Potsdam
2006-2019
Institut für biologische Forschung
1997-2019
Institut für Seeverkehrswirtschaft und Logistik
2018
University of Glasgow
2013-2017
Institute for Molecular Medicine
2016
Glasgow Life
2014-2016
University of Freiburg
2012-2014
Humboldt-Universität zu Berlin
1997-2012
National Agricultural Technology Institute
2009
The vesicle-inducing protein in plastids (VIPP1) was suggested to play a role thylakoid membrane formation via vesicles. As this functional assignment is under debate, we investigated the function of VIPP1 Chlamydomonas reinhardtii. Using immunofluorescence, localized distinct spots within chloroplast. In VIPP1-RNA interference/artificial microRNA cells, consistently observed aberrant, prolamellar body-like structures at origin multiple layers, which appear coincide with immunofluorescent...
Abstract Little is known so far about RNA regulators of photosynthesis in plants, algae, or cyanobacteria. The small PsrR1 (formerly SyR1) has been discovered Synechocystis sp PCC 6803 and appears to be widely conserved within the cyanobacterial phylum. Expression induced shortly after a shift from moderate high-light conditions. Artificial overexpression led bleaching phenotype under light growth Advanced computational target prediction suggested that several photosynthesis-related mRNAs...
Xanthophyll-cycle kinetics as well the relationship between xanthophyll de-epoxidation state and Stern-Volmer type nonphotochemical chlorophyll (Chl) fluorescence quenching (qN) were investigated in barley (Hordeum vulgare L.) leaves comprising a stepwise reduced antenna system. For this purpose plants of wild (WT) Chl b-less mutant chlorina 3613 cultivated under either continuous (CL) or intermittent light (IML). Violaxanthin (V) availability varied from about 70% WT up to 97 98% IML-grown...
A 3D inverse-opal mesoporous scalable electrode utilizing photosystem I with high efficiency for photocurrent generation and providing insights into protein-surface electrochemistry.
We report on the development of graphene-nanobiohybrid light-harvesting electrodes consisting photosystem I and π-system modified graphene electrodes.
We report on the development of an all-biomolecule-based light harvesting 3D architecture electrodes allowing efficient and tunable photocurrent generation.
Abstract The glycerolipid digalactosyl diacylglycerol (DGDG) is exclusively associated with photosynthetic membranes and thus may play a role in the proper assembly maintenance of apparatus. Here we employ genetic approach based on dgd1 mutant Arabidopsis thaliana to investigate function DGDG thylakoid membranes. primary defect genetically well-characterized resulted 90% reduction content. showed decreased photosystem II (PSII) I ratio. In vivo room- low-temperature (77 K) chlorophyll...
Summary Extremophilic organisms are gaining increasing interest because of their unique metabolic capacities and great biotechnological potential. The unicellular acidophilic mesothermophilic red alga Galdieria sulphuraria (074G) can grow autotrophically in light as well heterotrophically the dark. In this paper, effects externally added glucose on primary secondary photosynthetic reactions assessed to elucidate mixotrophic alga. Photosynthetic O 2 evolution was quantified an open system...
Conversion of light into an electrical current based on biohybrid systems mimicking natural photosynthesis is becoming increasingly popular. Photosystem I (PSI) particularly useful in such photo-bioelectrochemical devices. Herein, we report a novel biomimetic approach for effective assembly photosystem with the electron transfer carrier cytochrome c (cyt c), deposited thiol-modified gold-surface. Atomic force microscopy and surface plasmon resonance measurements have been used...
Artificial light-driven signal chains are particularly important for the development of systems converting light into a current, chemicals or light-induced sensing. Here, we report on construction an all-protein, light-triggered, catalytic circuit based photosystem I, cytochrome c (cyt c) and human sulfite oxidase (hSOX). The defined assembly all components using modular design results in artificial biohybrid electrode architecture, combining photophysical features PSI with biocatalytic...
The trimeric main light-harvesting complex (LHC-II) is the only antenna of higher plants which a high-resolution 3D structure has been obtained (Kühlbrandt, W., Wang, D., and Fujiyoshi, Y. (1994) Nature 367, 614−621) can be refolded in vitro from its components. Four different recombinant forms LHC-II, each with specific chlorophyll (Chl) binding site removed by site-directed mutagenesis, were heterologously overexpressed apoprotein, purified pigments, lipid. Absorption spectra mutant LHC-II...
Ycf53 is a hypothetical chloroplast open reading frame with similarity to the Arabidopsis nuclear gene GUN4 . In plants, involved in tetrapyrrole biosynthesis. We demonstrate that one of two Synechocystis sp. PCC 6803 ycf53 genes functions chlorophyll (Chl) biosynthesis as well: cyanobacterial gun4 mutant cells exhibit lower Chl contents, accumulate protoporphyrin IX and show less activity not only Mg chelatase but also Fe chelatase. The possible role Gun4 for well porphyrin branches discussed.
A large, two-orders-of-magnitude enhancement of fluorescence emission from cyanobacterial Photosystem I (PSI) coupled to plasmonic excitations in silver island films was observed. Such a high value has not been reported for metal-enhanced photosynthetic pigment-protein complexes before. The dramatic the PSI occurs when is excited resonantly into Qx and Qy bands chlorophyll a. In contrast, excitation carotenoid absorption band yields ten times lower factors. We attribute these large values...
Water-soluble chlorophyll-binding proteins (WSCPs) are interesting model systems for the study of pigment-pigment and pigment-protein interactions. While class IIa WSCP has been extensively studied by spectroscopic theoretical methods, a comprehensive IIb was lacking so far despite fact that its structure determined X-ray crystallography. In this paper, results two-dimensional electronic spectroscopy applied to from Lepidium virginicum presented. Global analysis 2D data allowed determination...
One major vital element of the oxygenic photosynthesis is photosystem I (PSI). We report on construction graphene-based nanohybrid light-harvesting architectures consisting PSI supercomplexes adsorbed onto π-system-modified graphene interfaces. The light-driven nanophotobioelectrochemical have been designed a modified carbon surface, basis π-π-stacking interactions between polycyclic aromatic compounds and graphene. As result remarkable features feasibility purposeful surface property...
Abstract A cytoplasmically inherited chlorophyll-deficient mutant of barley (Hordeum vulgare) termed cytoplasmic line 3 (CL3), displaying a viridis (homogeneously light-green colored) phenotype, has been previously shown to be affected by elevated temperatures. In this article, biochemical, biophysical, and molecular approaches were used study the CL3 under different temperature light conditions. The results lead conclusion that an impaired assembly photosystem I (PSI) higher temperatures...
Prochlorococcus marinus SS120 is an ecologically important and biochemically intriguing marine cyanobacterium. In addition to divinyl chlorophylls (DV‐Chls) a b it possesses particular form of phycoerythrin (PE), but no other phycobilins therefore complete phycobilisomes. Here, spectroscopic characterisation these DV‐Chls PE provided. Comparison fluorescence quantum yields, excited state lifetimes absorption characteristics indicate similar light‐harvesting properties the as their monovinyl...