A5006500541- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Herpesvirus Infections and Treatments
- Galectins and Cancer Biology
- Viral Infections and Immunology Research
- CAR-T cell therapy research
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- Macrophage Migration Inhibitory Factor
- interferon and immune responses
- Signaling Pathways in Disease
- RNA Interference and Gene Delivery
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Pancreatic and Hepatic Oncology Research
- Glycosylation and Glycoproteins Research
- Ocular Diseases and Behçet’s Syndrome
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Respiratory viral infections research
- Mosquito-borne diseases and control
- Antimicrobial Peptides and Activities
- Cardiovascular and exercise physiology
Umeå University
2015-2025
Johns Hopkins University
2010
Hohenstein Institute
2010
Medizinische Hochschule Hannover
2010
Johns Hopkins Medicine
2010
European Molecular Biology Laboratory
2009
National Center for Immunization and Respiratory Diseases
2008
Centers for Disease Control and Prevention
2008
The family Adenoviridae includes non-enveloped viruses with linear dsDNA genomes of 25–48 kb and medium-sized icosahedral capsids. Adenoviruses have been discovered in vertebrates from fish to humans. is divided into six genera, each which more common certain animal groups. outcome infection may vary subclinical lethal disease. This a summary the ICTV Report on , available at ictv.global/report/adenoviridae .
ABSTRACT The 51 human adenovirus serotypes are divided into six species (A to F). Many adenoviruses use the coxsackie-adenovirus receptor (CAR) for attachment host cells in vitro. Species B do not compete with CAR-binding binding cells, and it has been suggested that a other than CAR. mainly cause disease respiratory tract, eyes, urinary tract. Here we demonstrate type 11 (Ad11; of B) binds Chinese hamster ovary (CHO) transfected CD46 (membrane cofactor protein)-cDNA at least 10 times more...
Two cellular receptors for adenovirus, coxsackievirus-adenovirus receptor (CAR) and major histocompatibility complex class I (MHC-I) alpha2, have recently been identified. In the absence of CAR, MHC-I alpha2 has suggested to serve as a attachment protein subgenus C adenoviruses, while members from all subgenera except B shown interact with CAR. We found that adenovirus type 37 (Ad37) CAR-expressing CHO cells was no better than lacking CAR expression, suggesting is not used by Ad37 during...
Adenovirus serotype 37 (Ad37) belongs to species D and can cause epidemic keratoconjunctivitis, whereas the closely related Ad19p does not. Primary cell attachment by adenoviruses is mediated through receptor binding of knob domain fiber protein. The knobs Ad37 differ at only two positions, Lys240Glu Asn340Asp. We report high-resolution crystal structures knobs, both native in complex with sialic acid, which has been proposed as a for Ad37. Overall, are very similar previously reported...
The 51 human adenovirus serotypes are divided into six species (A to F). Adenovirus from all except B utilize the coxsackie-adenovirus receptor for attachment host cells in vitro. Species adenoviruses primarily cause ocular and respiratory tract infections, but certain also associated with renal disease. We have previously demonstrated that type 11 (species B) uses CD46 (membrane cofactor protein) as a cellular instead of (A. Segerman et al., J. Virol. 77:9183-9191, 2003). In present study,...
ABSTRACT Selected members of the adenovirus family have been shown to interact with coxsackie receptor, α v integrins, and sialic acid on target cells. Initial interactions subgenus D adenoviruses cells until now poorly characterized. Here, we demonstrate that type 8 (Ad8), Ad19a, Ad37 use as a functional cellular whereas Ad9 Ad19 prototypes do not.
The recently discovered human Merkel cell polyomavirus (MCPyV or MCV) causes the aggressive carcinoma (MCC) in skin of immunocompromised individuals. Conflicting reports suggest that cellular glycans containing sialic acid (Neu5Ac) may play a role MCPyV infectious entry. To address this question, we solved X-ray structures major capsid protein VP1 both alone and complex with several sialylated oligosaccharides. A shallow binding site on apical surface capsomer recognizes disaccharide...
Most adenoviruses attach to host cells by means of the protruding fiber protein that binds via coxsackievirus and adenovirus receptor (CAR) protein. Human type 52 (HAdV-52) is one only three gastroenteritis-causing HAdVs are equipped with two different proteins, long short. Here we show, virion-cell binding infection experiments, HAdV-52 can also CAR, but most depends on sialylated glycoproteins. Glycan microarray, flow cytometry, surface plasmon resonance ELISA analyses reveal terminal knob...
Human adenovirus 52 (HAdV-52) is one of only three known HAdVs equipped with both a long and short fiber protein. While the binds to coxsackie receptor, function in virus life cycle poorly understood. Here, we show, by glycan microarray analysis cellular studies, that knob (SFK) HAdV-52 recognizes chains α-2,8-linked polysialic acid (polySia), large posttranslational modification selected carrier proteins, can use polySia as receptor on target cells. X-ray crystallography, NMR, molecular...
Significance The adenovirus capsid protein is built by three main capsomers: hexon, fiber, and penton base. Entry mediated fiber proteins anchoring the virus to host cell receptors followed base engaging coreceptors, resulting in entry. Here, we demonstrate that human species D types, which constitute two-thirds of all adenoviruses, enter cells through a direct interaction between hexon CD46. This study provides insights into entry mechanisms used adenoviruses. As these viruses are also as...
Human adenovirus (HAdV) types F40 and F41 are a prominent cause of diarrhea diarrhea-associated mortality in young children worldwide. These enteric HAdVs differ notably tissue tropism pathogenicity from respiratory ocular adenoviruses, but the structural basis for this divergence has been unknown. Here, we present first structure an HAdV-HAdV-F41-determined by cryo-electron microscopy to resolution 3.8 Å. The reveals extensive alterations virion exterior as compared nonenteric HAdVs,...
ABSTRACT Unlike most adenovirus (Ad) serotypes, the species B Ads do not use coxsackie-adenovirus receptor as an attachment receptor. The receptor(s) has yet been identified and is also poorly characterized. Species can be further divided into B1 B2 Ads, these display different organ tropisms, suggesting a difference in usage. We have studied interactions of serotypes 3p 7p 11p 35 characterized properties receptor(s). Reciprocal blocking experiments using unlabeled Ad11p or Ad3p virions to...
ABSTRACT Binding to target cell receptors is a critical step in the virus life cycle. Coxsackievirus A24 variant (CVA24v) has pandemic potential and major cause of acute hemorrhagic conjunctivitis, but its cellular receptor hitherto been unknown. Here we show that CVA24v fails bind infect CHO cells defective sialic acid expression. infection corneal epithelial are efficiently inhibited by treating with acid-cleaving enzyme or acid-binding lectins treatment soluble, multivalent acid. Protease...
Significance Acute hemorrhagic conjunctivitis (AHC) is a painful and highly contagious infection of the eye, with reported incidence rates up to 48%. No drugs or vaccines are available for treatment prevention AHC. Coxsackievirus A24 variant (CV-A24v) main etiological agent AHC, being responsible >10 million AHC cases worldwide during last decades. We have identified CV-A24v protein receptor determined high-resolution structure virus–receptor complex. Furthermore, we found that an...
Human adenovirus type 36 (HAdV-D36) has been putatively linked to obesity in animals and associated with humans some but not all studies. Despite extensive epidemiological research there is limited information about its receptor profile. We investigated the portfolio of HAdV-D36 using a combined structural biology virology approach. The fiber knob domain (FK), which mediates primary attachment many HAdVs host cells, significantly elongated DG loop that alters known binding interfaces for...
ABSTRACT Epidemic keratoconjunctivitis (EKC) is a severe eye infection caused mainly by adenovirus type 8 (Ad8), Ad19, and Ad37. We have shown that the EKC-causing adenoviruses use sialic acid as cellular receptor on A549 cells instead of coxsackie-adenovirus receptor, which used most adenoviruses. Recently, Wu et al. (Virology 279:78-89, 2001) proposed Ad37 uses 50-kDa protein Chang C conjunctival this interaction independent acid. According to American Type Culture Collection, cell line...
Most adenoviruses bind to the coxsackie- and adenovirus receptor (CAR). Surprisingly, CAR is not expressed apically on polarized cells thus easily available viruses. Consequently, alternative mechanisms for entry of coxsackievirus into have been suggested. We found that tear fluid promotes infection, we identified human lactoferrin (HLf) as component responsible this effect. HLf alone was promote binding epithelial in a dose-dependent manner also infection by adenovirus. gene delivery from...