A5082437028- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- Polyomavirus and related diseases
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Cellular transport and secretion
- Peptidase Inhibition and Analysis
- Viral Infections and Immunology Research
- MicroRNA in disease regulation
- Viral gastroenteritis research and epidemiology
- Mechanisms of cancer metastasis
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- FOXO transcription factor regulation
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Extracellular vesicles in disease
- Genetic factors in colorectal cancer
- Receptor Mechanisms and Signaling
- Endoplasmic Reticulum Stress and Disease
- Cell Adhesion Molecules Research
- RNA Research and Splicing
University of Tübingen
2015-2025
Utrecht University
2018-2023
ABSTRACT Extracellular vesicles (EV) are membrane encapsulated nanoparticles that can function in intercellular communication, and their presence biofluids be indicative for (patho)physiological conditions. Studies aiming to resolve functionalities of EV or discover EV‐associated biomarkers disease liquid biopsies hampered by limitations current protocols isolate from cell culture medium. isolation is complicated the >10 5 ‐fold numerical excess other types particles, including...
Human adenovirus 52 (HAdV-52) is one of only three known HAdVs equipped with both a long and short fiber protein. While the binds to coxsackie receptor, function in virus life cycle poorly understood. Here, we show, by glycan microarray analysis cellular studies, that knob (SFK) HAdV-52 recognizes chains α-2,8-linked polysialic acid (polySia), large posttranslational modification selected carrier proteins, can use polySia as receptor on target cells. X-ray crystallography, NMR, molecular...
Most adenoviruses attach to host cells by means of the protruding fiber protein that binds via coxsackievirus and adenovirus receptor (CAR) protein. Human type 52 (HAdV-52) is one only three gastroenteritis-causing HAdVs are equipped with two different proteins, long short. Here we show, virion-cell binding infection experiments, HAdV-52 can also CAR, but most depends on sialylated glycoproteins. Glycan microarray, flow cytometry, surface plasmon resonance ELISA analyses reveal terminal knob...
Human adenovirus type 36 (HAdV-D36) has been putatively linked to obesity in animals and associated with humans some but not all studies. Despite extensive epidemiological research there is limited information about its receptor profile. We investigated the portfolio of HAdV-D36 using a combined structural biology virology approach. The fiber knob domain (FK), which mediates primary attachment many HAdVs host cells, significantly elongated DG loop that alters known binding interfaces for...
Adenovirus type 37 (Ad37) is one of the principal agents responsible for epidemic keratoconjunctivitis (EKC), a severe ocular infection that remains without treatment.
Abstract The DNA mismatch repair protein MutSα recognizes wrongly incorporated bases and initiates their correction during replication. Dysfunctions in lead to a predisposition cancer. Here, we study the homozygous mutation V63E MSH2 that was found germline of patient with suspected constitutional deficiency syndrome who developed colorectal cancer before age 30. Characterization mutant mouse models, as well slippage assays, shows mildly pathogenic phenotype. Using cryogenic electron...
Murine polyomavirus (MuPyV) causes tumors of various origins in newborn mice and hamsters. Infection is initiated by attachment the virus to ganglioside receptors at cell surface. Single amino acid exchanges receptor-binding pocket major capsid protein VP1 are known drastically alter tumorigenicity spread closely related MuPyV strains. The represents a rare example differential receptor recognition directly influencing viral pathogenicity, although factors underlying these differences remain...
Human adenovirus serotype 26 (Ad26) is used as a gene-based vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and HIV-1. However, its primary receptor portfolio remains controversial, potentially including sialic acid, coxsackie (CAR), integrins, CD46. We others have shown that Ad26 can use CD46, but these observations were questioned on the basis of inability to cocrystallize fiber with Recent work demonstrated binds CD46 hexon protein rather than fiber. examined...
The signal peptidase complex (SPC) is an essential membrane in the endoplasmic reticulum (ER), where it removes peptides (SPs) from a large variety of secretory pre-proteins with exquisite specificity. Although determinants this process have been established empirically, molecular details SP recognition and removal remain elusive. Here, we show that human SPC exists two functional paralogs distinct proteolytic subunits. We determined atomic structures both using electron cryo-microscopy...
Abstract The signal peptidase complex (SPC) is an essential membrane in the endoplasmic reticulum (ER), where it removes peptides (SPs) from a large variety of secretory pre-proteins with exquisite specificity. Although determinants this process have been established empirically, molecular details SP recognition and removal remain elusive. Here, we show that human SPC exists two functional paralogs distinct proteolytic subunits. We determined atomic structures both using electron...
ABSTRACT Human adenovirus serotype 26 (Ad26) is used as a gene-based vaccine against SARS-CoV-2 and HIV-1. Yet, its primary receptor portfolio remains controversial, potentially including sialic acid, CAR, integrins, CD46. We others have shown that Ad26 can use CD46, but these observations were questioned by the inability to co-crystallize fiber with Recent work demonstrated binds CD46 hexon protein rather than fiber. examined functional consequences of for infection in vitro vivo . Ectopic...