A5103090017- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- RNA regulation and disease
- Identification and Quantification in Food
- CAR-T cell therapy research
- Viral Infections and Outbreaks Research
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Folate and B Vitamins Research
- Vector-Borne Animal Diseases
- Heat shock proteins research
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Endoplasmic Reticulum Stress and Disease
- Viral Infections and Vectors
- Fungal and yeast genetics research
- Hepatitis Viruses Studies and Epidemiology
- Fish Biology and Ecology Studies
- Meat and Animal Product Quality
- Genetic diversity and population structure
- Lymphatic System and Diseases
- Biochemical and Structural Characterization
- Respiratory viral infections research
- T-cell and Retrovirus Studies
Swedish Veterinary Agency
2021-2023
Aberystwyth University
2023
Umeå University
2017-2021
Indian Veterinary Research Institute
2017
National Bureau of Fish Genetic Resources
2014
University of California, Santa Barbara
2013-2014
Central Marine Fisheries Research Institute
2013
University of Rostock
2010-2013
Charité - Universitätsmedizin Berlin
2012
Institute of Genomics and Integrative Biology
2006-2011
Significance The adenovirus capsid protein is built by three main capsomers: hexon, fiber, and penton base. Entry mediated fiber proteins anchoring the virus to host cell receptors followed base engaging coreceptors, resulting in entry. Here, we demonstrate that human species D types, which constitute two-thirds of all adenoviruses, enter cells through a direct interaction between hexon CD46. This study provides insights into entry mechanisms used adenoviruses. As these viruses are also as...
Approaches are needed for therapy of the severe acute respiratory syndrome from SARS-CoV-2 coronavirus (COVID-19). Interfering with interaction viral antigens angiotensin converting enzyme 2 (ACE-2) receptor is a promising strategy by blocking infection coronaviruses into human cells. We have implemented novel protein engineering technology to produce super-potent tetravalent form ACE2, coupled immunoglobulin γ1 Fc region, using self-assembling, tetramerization domain p53 protein. This high...
Intracellular thiols like cysteine, homocysteine and glutathione play a critical role in the regulation of important cellular processes. Alteration intracellular thiol concentration results many diseased states; for instance, elevated levels are considered to be an independent risk factor cardiovascular disease. Yeast has proved excellent model system studying human diseases since it carries homologues nearly 40% disease genes fundamental pathways highly conserved between two organisms. In...
Chikungunya virus (CHIKV) is an alphavirus of the Togaviridae family. After autoproteolytic cleavage, CHIKV capsid protein (CP) involved in RNA binding and assembly viral particle. The monomeric CP approximately 30 kDa size small enough for passive transport through nuclear pores. Some alphaviruses are found to harbor localization signals (NLS) these proteins between cellular compartments was shown be energy dependent. active import cytoplasmic mediated by karyopherins their export...
Hepatitis E virus (HEV) is a non-enveloped plus-strand RNA that causes acute hepatitis. The capsid protein open reading frame 2 (ORF2) known to induce endoplasmic reticulum stress in ORF2 expressing cells.In this study we found HEV activates the expression of pro-apoptotic gene C/EBP homologous (CHOP). stimulates CHOP promoter mainly through AARE (amino acid response elements) and minor extent ERSE (endoplasmic elements). Activating transcription factor 4 (ATF4) binds regulatory sites...
Marburg and Ebola filoviruses are two of the deadliest infectious agents several outbreaks have occurred in last decades. Although receptors co-receptors been reported for virus, key host factors remain to be elucidated. In this study, using a haploid cell screening platform, we identify guanine nucleotide exchange factor CCZ1 as early stage filovirus replication. The critical role infections is validated 3D primary human hepatocyte cultures blood-vessel organoids, both target sites virus...
The alphavirus capsid is multifunctional and plays a key role in the viral life cycle. nucleocapsid domain released by self-cleavage activity of serine protease within capsid. All alphaviruses analyzed to date show this autocatalytic cleavage. Here we have sequence requirements for cleavage Chikungunya virus genus alphavirus. Amongst alphaviruses, C-terminal amino acid tryptophan (W261) conserved found be important Mutating alanine (W261A) completely inactivated protease. Other acids near...
An elevated level of homocysteine, a thiol amino acid, is associated with various complex disorders. The cellular effects homocysteine and its precursors S-adenosylhomocysteine (AdoHcy) S-adenosylmethionine (AdoMet) are, however, poorly understood. We used Saccharomyces cerevisiae as model to understand the basis pathogenicity induced by precursors. Both AdoHcy but not AdoMet inhibited growth str4Δ strain (which lacks enzyme that converts cystathionine-mimicking vascular cells). Addition...
The vectorization of rare human adenovirus (HAdV) types will widen our knowledge this family and their interaction with cells, tissues organs. In study we focus on HAdV-56, a member Ad species D, create ease-of-use cloning systems to generate recombinant HAdV-56 vectors carrying foreign genes. We present in vitro transduction profiles for direct comparison the most commonly used HAdV-5-based vector. vivo characterizations demonstrate that when it is delivered intravenously (i.v.) mainly...
Preexisting immune responses toward adenoviral vectors limit the use of a vector based on particular serotypes and its clinical applicability for gene therapy and/or vaccination. Therefore, there is significant interest in vectorizing novel types that have low seroprevalence human population. Here, we describe discovery vectorization chimeric adenovirus, which call HAdV-20-42-42. Full-genome sequencing revealed this virus closely related to serotype 42, except penton base, derived from 20....
Abstract Approaches are needed for therapy of the severe acute respiratory syndrome from SARS-CoV-2 coronavirus (COVID-19). Interfering with interaction viral antigens angiotensin converting enzyme 2 (ACE-2) receptor is a promising strategy by blocking infection coronaviruses into human cells. We have implemented novel protein engineering technology to produce super-potent tetravalent form ACE2, coupled immunoglobulin g1 Fc region, using self-assembling, tetramerization domain p53 protein....
Bluetongue virus (BTV), an arbovirus of ruminants, is a causative agent numerous epidemics around the world. Due to emergence novel reassortant BTV strains and new outbreaks, there unmet need for efficacious antivirals. In this study, we used improved haploid screening platform identify relevant host factors infection. Our tool identified validated factor Niemann-Pick C1 (NPC1), lysosomal membrane protein that involved in cholesterol transport, as critical This finding prompted us...
Red lined torpedo barbs (RLTBs) (Cyprinidae: Puntius) endemic to the Western Ghats Hotspot of India, are popular and highly priced freshwater aquarium fishes.Two decades indiscriminate exploitation for pet trade, restricted range, fragmented populations continuing decline in quality habitats has resulted their 'Endangered' listing.Here, we tested whether isolated RLTB demonstrated considerable variation qualifying be considered as distinct conservation targets.Multivariate morphometric...
Interleukin 28B (IL28B) which is also called as Interferon lambda3 (IFNλ3) has been analyzed for its immunomodulatory activity in mice. Bovine IL28B protein was administered to mice intramuscularly and then the spleenocytes were harvested from on 7th day post injection. The collected activation of B T lymphocytes using Fluorescent Activated Cell Sorting (FACS) analysis. Helper cell CD3-FITC, CD4-PE/Cy7 CD69-PE; cytotoxic CD8-PE/Cy7 CD69-PE CD19-PE CD22-FITC studied. results show that...
Type III Interferons also known as Interferon lambdas (IFNï¬s/IFNLs) constitute a recently described IFN with antiviral activities by signaling through unique receptor complex composed of IFNï¬s 1 (IFNï¬R1) and interleukin-10 2 (IL10R2). It found to have more immune modulatory in addition activities. In the present study, construct pcDNA-bovine IFNλ3 was administered intramuscularly mice. The mice were sacrificed on 7th 14th day collect different organs/tissues. tissues used isolate total...
The islands of the Caribbean are particularly susceptible to effects climate change due their low-lying coastal areas and location within Atlantic basin's hurricane belt. UK Overseas Territory Anguilla is one such island. predicted increase in severity hurricanes sea-level rise highly likely flood risk already vulnerable island communities. In this study, erosion models used prioritise opportunity for nature-based restoration identify those that would have greatest impact on in-land...