A5070883421- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Fire effects on ecosystems
- SARS-CoV-2 and COVID-19 Research
- Malaria Research and Control
- Plant Virus Research Studies
- COVID-19 Clinical Research Studies
- Urinary Tract Infections Management
- interferon and immune responses
- Viral Infections and Immunology Research
- Reproductive tract infections research
- Wheat and Barley Genetics and Pathology
- Plant and fungal interactions
- Energy and Environment Impacts
- SARS-CoV-2 detection and testing
Karolinska Institutet
2021-2024
Karolinska University Hospital
2021-2024
Public Health Agency of Sweden
2024
Justus-Liebig-Universität Gießen
2016-2023
Philipps University of Marburg
2012-2017
Biomedical Research Institute
2010
Institut de Médecine Tropicale du Service de Santé des Armées
2010
Institut de Virologie
2010
ABSTRACT Crimean-Congo hemorrhagic fever virus (CCHFV) is a bunyavirus causing severe disease in humans, with high mortality rates. The requirement of high-containment laboratory and the lack an animal model hampered study immune response protection vaccine candidates. Using recently developed interferon alpha receptor knockout (IFNAR −/− ) mouse model, which replicates human disease, we investigated immunogenicity two novel CCHFV candidates: DNA encoding ubiquitin-linked version Gc, Gn, N...
Article4 July 2022Open Access Transparent process Clinical grade ACE2 as a universal agent to block SARS-CoV-2 variants Vanessa Monteil orcid.org/0000-0002-2652-5695 Unit of Microbiology, Karolinska Institutet and University Hospital, Stockholm, Sweden Contribution: Conceptualization, Data curation, Formal analysis, Visualization, Methodology Search for more papers by this author Brett Eaton NIAID Integrated Research Facility at Fort Detrick (IRF-Frederick), Frederick, Maryland, USA analysis...
Abstract Climate change and population densities accelerated transmission of highly pathogenic viruses to humans, including the Crimean–Congo haemorrhagic fever virus (CCHFV). Here we report that Low Density Lipoprotein Receptor (LDLR) is a critical receptor for CCHFV cell entry, playing vital role in infection culture blood vessel organoids. The interaction between LDLR specific, with other members protein family failing bind or neutralize virus. Biosensor experiments demonstrate...
Abstract Chlamydia trachomatis is a global cause of blinding trachoma and sexually transmitted infections (STIs). We used comparative genomics the family Chlamydiaceae to select conserved housekeeping genes for C. multilocus sequencing, characterizing 19 reference 68 clinical isolates from 6 continental/subcontinental regions. There were 44 sequence types (ST). Identical STs STI recovered different regions, whereas restricted by continent. Twenty-nine 52 alleles had nonuniform distributions...
ABSTRACT Crimean-Congo hemorrhagic fever, a severe disease found throughout Africa, Europe, and Asia, is caused by the tick-borne fever virus (CCHFV). CCHFV negative-sense single-stranded RNA (ssRNA) belonging to Nairovirus genus of Bunyaviridae family. Its genome three segments encapsidated nucleocapsid protein (CCHFV N) form ribonucleoprotein complex. This complex required during replication transcription viral genomic RNA. Here, we present crystal structures N in two distinct forms, an...
Viruses with an RNA genome are often the cause of zoonotic infections. In order to identify novel pro-viral host cell factors, we screened a haploid insertion–mutagenized mouse embryonic library for clones that resistant Rift Valley fever virus (RVFV). This screen returned low-density lipoprotein receptor–related protein 1 (LRP1) as top hit, plasma membrane involved in wide variety activities. Inactivation LRP1 human cells reduced RVFV levels already at attachment and entry stages infection....
ABSTRACT Crimean-Congo hemorrhagic fever virus (CCHFV; genus Nairovirus ) is an extremely pathogenic member of the Bunyaviridae family. Since handling requires a biosafety level 4 (BSL-4) facility, little known about pathomechanisms and host interactions. Here, we describe establishment transcriptionally competent virus-like particle (tc-VLP) system for CCHFV. Recombinant polymerase (L), nucleocapsid protein (N) reporter minigenome expressed in human HuH-7 cells resulted formation active...
Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne orthonairovirus that has become serious threat to the public health. CCHFV single-stranded, tripartite RNA genome composed of L, M, and S segments. Cleavage M polyprotein precursor generates two envelope glycoproteins (GPs) as well three secreted nonstructural proteins GP38 GP85 or GP160, representing only linked mucin-like protein (MLD), double-membrane-spanning called NSm. Here, we examined relevance each M-segment...
Background Deciphering host responses contributing to dengue shock syndrome (DSS), the life-threatening form of acute viral infections, is required improve both differential prognosis and treatments provided DSS patients, a challenge for clinicians. Methodology/Principal Findings Based on prospective study, we analyzed genome-wide expression profiles whole blood cells from 48 matched Cambodian children: 19 progressed while 16 13 presented respectively classical fever (DF) or hemorrhagic...
Abstract Crimean Congo hemorrhagic fever virus (CCHFV) is a highly virulent tick-borne pathogen that causes in humans. The geographic range of human CCHF cases largely reflects the presence ticks. However, similar CCHFV lineages occur geographically distant regions. Tick-infested migratory birds have been suggested, but not confirmed, to contribute dispersal. Bats recently shown carry nairoviruses distinct from CCHFV. In order assess wide bat species over range, we analyzed 1,135 sera 16...
Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome wrapped around virally encoded nucleoprotein N to form ribonucleoprotein complex (RNP). We determined crystal structures hexameric alone (apo) and with a nonameric single-stranded RNA. RNA sequestered sequence-independent fashion deep groove inside hexamer. At junction between two adjacent copies Ns,...
Marburg and Ebola filoviruses are two of the deadliest infectious agents several outbreaks have occurred in last decades. Although receptors co-receptors been reported for virus, key host factors remain to be elucidated. In this study, using a haploid cell screening platform, we identify guanine nucleotide exchange factor CCZ1 as early stage filovirus replication. The critical role infections is validated 3D primary human hepatocyte cultures blood-vessel organoids, both target sites virus...
Toscana virus (TOSV) is an arthropod-borne human pathogen responsible for seasonal outbreaks of fever and meningoencephalitis in the Mediterranean basin. TOSV a segmented negative-strand RNA (sNSV) that belongs to genus phlebovirus (family Phenuiviridae, order Bunyavirales), encompassing other important pathogens such as Rift Valley (RVFV). Here, we carried out structural functional characterization cap-snatching endonuclease, N terminal domain viral polymerase (L protein) provides capped...
Background Many ruminant diseases of viral aetiology can be effectively prevented using appropriate vaccination measures. For such as Rift Valley fever (RVF) the long inter-epizootic periods make routine programs unfeasible. Coupling RVF prophylaxis with seasonal programmes by means multivalent vaccine platforms would help to reduce risk new outbreaks. Methodology/Principal findings In this work we generated recombinant attenuated viruses (RVFVs) encoding in place virulence factor NSs either...
Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne orthonairovirus that causes severe, often fatal, disease throughout Africa, Asia, and Southeast Europe. A wide variety of strains are circulating in the field which broadly correlate to their geographic distribution. The viral determinants pathogenicity remain unclear, as does contribution strain-specific differences pathology. Aigai (AIGV) closely related (formally designated CCHFV genotype VI, Europe II, or AP92-like virus), has...
Dear Editor,Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne viral disease widely distributed across countries of Africa, Southern Europe, the Middle East, and Asia1. CCHF caused...
Crimean-Congo Hemorrhagic Fever virus (CCHFV; family Nairoviridae) is an extremely pathogenic member of the Bunyavirales order. Previous studies have shown that N-terminal domain CCHFV polymerase (L) contains ovarian tumor-type protease (OTU) with capability to remove both ubiquitin and ISG15 molecules from proteins. The approximately 200 amino acids-long OTU domain, if ectopically expressed, can interfere induction antiviral type I interferons (IFN) as well IFN-stimulated signaling. A...
Abstract The recent emergence of the SARS-CoV-2 variant Omicron has caused considerable concern due to reduced vaccine efficacy and escape from neutralizing antibody therapeutics. is spreading rapidly around globe suspected account for most new COVID-19 cases in several countries, though severity Omicron-mediated disease still under debate. It therefore paramount identify therapeutic strategies that inhibit variant. Here we report using 3D structural modelling Spike can associate with human...
Abstract Viruses with an RNA genome are often the cause of zoonotic infections. In order to identify novel pro-viral host cell factors, we screened a haploid insertion-mutagenized mouse embryonic library for clones that rendered them resistant Rift Valley fever virus (RVFV; family Phleboviridae , Bunyavirales ). This screen returned Low Density Lipoprotein Receptor-Related protein 1 (LRP1, or CD91) as top hit, 600 kDa plasma membrane known be involved in wide variety activities. Inactivation...
Purpose: Rift Valley fever virus (RVFV, family Bunyaviridae, genus Phlebovirus) is currently referenced by the WHO as one of 8 emerging pathogens likely to cause serious outbreaks in near future and for which few medical countermeasures exist. Transmitted mosquitoes, RVFV causes large devastating epidemics Africa Arabia, leading hemorrhages massive abortions livestock ruminants, hepatitis 10-20% human hospitalized patients. While some proteins are known interfere with apoptosis antiviral...