A5053471856- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Viral Infections and Outbreaks Research
- HIV/AIDS Research and Interventions
- Viral Infections and Vectors
- Hepatitis B Virus Studies
- Gastrointestinal motility and disorders
- RNA Research and Splicing
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Advanced Glycation End Products research
- Influenza Virus Research Studies
- HIV-related health complications and treatments
- Virology and Viral Diseases
- Immunodeficiency and Autoimmune Disorders
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Renal and Vascular Pathologies
- Signaling Pathways in Disease
- Diabetes and associated disorders
- Data-Driven Disease Surveillance
- Biosensors and Analytical Detection
- Biochemical Acid Research Studies
- Dermatology and Skin Diseases
- Hepatitis C virus research
Public Health Agency of Sweden
2017-2024
Örebro University
2014-2020
Karolinska University Hospital
2004-2019
Maasai Mara University
2019
Helsinki University Hospital
2019
University of Nairobi
2019
Swedish Veterinary Agency
2019
University of Arkansas at Fayetteville
2019
University of Helsinki
2019
Karolinska Institutet
2005-2014
Bombali virus (genus Ebolavirus) was identified in organs and excreta of an Angolan free-tailed bat (Mops condylurus) Kenya. Complete genome analysis revealed 98% nucleotide sequence similarity to the prototype from Sierra Leone. No Ebola virus-specific RNA or antibodies were detected febrile humans area who reported contact with bats.
Abstract Climate change and population densities accelerated transmission of highly pathogenic viruses to humans, including the Crimean–Congo haemorrhagic fever virus (CCHFV). Here we report that Low Density Lipoprotein Receptor (LDLR) is a critical receptor for CCHFV cell entry, playing vital role in infection culture blood vessel organoids. The interaction between LDLR specific, with other members protein family failing bind or neutralize virus. Biosensor experiments demonstrate...
Abstract Background Innate immune responses to conserved microbial products such as lipopolysaccharide ( LPS ) and flagellin are likely important in microbial–host interactions intestinal homeostasis. We hypothesized that bacterial translocation activation of mucosal immunity against common antigens might be involved the development irritable bowel syndrome IBS ). therefore compared serum levels , soluble CD 14 sCD 14), antibodies between patients with different subtypes healthy controls....
Crimean-Congo hemorrhagic fever (CCHF), caused by virus (CCHFV), is on the World Health Organizations' list of prioritized diseases and pathogens. With global distribution, high fatality rate, no approved vaccine or effective treatment, CCHF constitutes a threat against health. In current study, we demonstrate that vaccination with nucleoside-modified mRNA-lipid nanoparticles (mRNA-LNP), encoding for CCHFV nucleoprotein (N) glycoproteins (GcGn) protect IFNAR-/- mice lethal infection....
Objective: To investigate plasma levels of high mobility group box-1 protein (HMGB1), a marker tissue necrosis and immune activation, as well lipopolysaccharide (LPS), bacterial translocation, in HIV-1-infected patients. Design: We studied 32 HIV-1-positive patients who had responded to antiretroviral therapy with undetectable viremia after 2 years, 10 nonresponders 19 healthy controls. Methods: HMGB1 was analyzed by ELISA, LPS Lamilus colometric assay. Nonparametric statistics were applied....
Emerging tropical viruses have caused serious outbreaks during the recent years, such as Ebola virus (EBOV) in 2014 and most 2018 to 2019 Congo. Thus, immediate diagnostic attention is demanded at point of care resource-limited settings, because performance operational parameters conventional EBOV testing are limited. Especially, their sensitivity, specificity, coverage other disease make them unsuitable for care. Here, a padlock probe (PLP)-based rolling circle amplification (RCA) method...
Baseline plasma samples of 490 randomly selected antiretroviral therapy (ART) naïve patients from seven hospitals participating in the first nationwide Ethiopian HIV-1 cohort were analysed for surveillance drug resistance mutations (sDRM) by population based Sanger sequencing (PBSS). Also next generation (NGS) was used a subset 109 baseline patients. Treatment outcome after 6- and 12-months assessed on-treatment (OT) intention-to-treat (ITT) analyses. Transmitted (TDR) detected 3.9% (18/461)...
Objectives We investigated whether there are differences in the effects on microbial translocation (MT) and enterocyte damage by different antiretroviral therapy (ART) regimens after 1.5 years antibiotic use has impact MT. In a randomized clinical trial (NCT01445223) first line ART, patients started either lopinavir/r (LPV/r) (n = 34) or efavirenz (EFV) containing ART 37). Lipopolysaccharide (LPS), sCD14, anti-flagellin antibodies intestinal fatty acid binding protein (I-FABP) levels were...
Plasma levels of high-mobility group box 1 protein (HMGB1) are elevated during the course human immunodeficiency virus type (HIV-1) infection and molecule has an impact on replication. This study investigated mode cell death release HMGB1 HIV-1 in vitro. MT4 cells primary CD4+ T were infected with isolates, was monitored relation to cytopathic effects (CPE) apoptosis. from analysed by Western blotting. For cells, enzyme-linked immunosorbent spot (ELISPOT) assay adapted measure necrosis....
Objective . We hypothesized that HMGB1 in complex with bacterial components, such as flagellin, CpG-ODN, and LPS, promotes HIV-1 replication. Furthermore, we studied the levels of antiflagellin antibodies during HIV-1-infection. Methods Chronically HIV-1-infected U1 cells were stimulated necrotic extract/recombinant TLR ligands or alone. replication was estimated by p24 antigen culture supernatants 48–72 hours after stimulation. The presence systemic anti-flagellin IgG determined 51 patients...
The capsid protein (CA or p24) of human immunodeficiency virus type 1 (HIV-1) plays a major role both early and late in the replication cycle. Many studies have suggested that C-terminal domain this is involved dimerization proper assembly viral core. Point mutations were introduced two conserved sites region their effects on expression, particle infectivity studied. Eight different mutants (L205A+P207A, L205A, P207A, 223GPG225AAA, G223A, P224A, G225A V221G) infectious clone pNL4-3...
Introduction The role of microbial translocation (MT) in HIV patients living with from low‐ and middle‐income countries (LMICs) is not fully known. aim this study to investigate compare the patterns MT Vietnam, Ethiopia Sweden. Methods Cross‐sectional samples were obtained treatment‐naïve HIV‐1 healthy controls Vietnam ( n =83; =46), =9492; =50) Sweden =51; =19). Longitudinal a subset Vietnamese =24) whom antiretroviral therapy (ART) tuberculostatics given. Plasma lipopolysaccharide (LPS),...
Abstract Background The mature HIV-1 conical core formation proceeds through highly regulated protease cleavage of the Gag precursor, which ultimately leads to substantial rearrangements capsid (CAp24) molecule involving both inter- and intra-molecular contacts CAp24 molecules. In this aspect, Asp51 is located in N-terminal domain plays an important role by forming a salt-bridge with free imino terminus Pro1 following proteolytic liberation protein from Pr55Gag precursor. Thus, previous...
CCR5 coreceptor using HIV-1 subtype C (HIV-1C) has been reported to dominate the Ethiopian epidemic. However, almost all data have obtained from two large cities in central and north-west regions recent is lacking.Plasma were 420 treatment-naïve patients recruited 2009-2011 a country-wide cohort. The V3 region was sequenced co-receptor tropism predicted by clinical clonal models of geno2pheno tool at different false positive rates (fpr) for subtype. In an intention treat analysis impact...
Abstract We have studied the effects associated with two single amino acid substitution mutations in HIV-1 capsid (CA), E98A and E187G. Both acids are well conserved among all major subtypes. infectivity is critically dependent on proper CA cone formation lethal when they inhibit assembly by destabilizing intra and/or inter molecular contacts, which ultimately abrogate viral replication. Glu98, located a surface of flexible cyclophilin A binding loop not involved any intra-molecular contacts...
To assess the presence of two major non-nucleoside reverse transcriptase inhibitors (NNRTI) drug resistance mutations (DRMs), Y181C and K103N, in minor viral quasispecies treatment naïve HIV-1 infected East-African Swedish patients by allele-specific polymerase chain reaction (AS-PCR).Treatment adults (n=191) with three epidemiological backgrounds were included: 92 Ethiopians living Ethiopia; 55 East-Africans who had migrated to Sweden; 44 Caucasians Sweden. The pol gene was analysed...
ABSTRACT Upon maturation of the human immunodeficiency virus type 1 (HIV-1) virion, proteolytic cleavage Gag precursor protein by viral protease is followed morphological changes capsid p24, which will ultimately transform core from an immature spherical to a mature conical structure. Virion infectivity critically dependent on optimal semistability cone We have reported earlier that glycineamide (G-NH 2 ), when added culture medium infected cells, inhibits HIV-1 replication and particles...
Abstract Background Formation of an HIV-1 particle with a conical core structure is prerequisite for the subsequent infectivity virus particle. We have previously described that glycineamide (G-NH 2 ) when added to culture medium infected cells induces non-infectious particles aberrant structures. Results Here we demonstrate it not G-NH itself but metabolite thereof displays antiviral activity. show conversion its catalyzed by enzyme present in bovine and porcine surprisingly human serum....
Summary Ebola virus glycoprotein is one of the most heavily O-glycosylated viral envelope glycoproteins. Using glycoengineered cell lines we demonstrate that O-linked glycan truncation and perturbed initiation retarded production particles decreased infectivity progeny virus. Next, using TMT isobaric labelling, performed quantitative differential O-glycoproteomics on proteins produced in wild type HEK293 cells ablated for three key GalNAc-transferases, GalNAc-T1, -T2, -T3, as well compared...
Genotypic tropism testing (GTT) has been developed largely on HIV-1 subtype B. Although a few reports have analysed the utility of GTT in other subtypes, more studies using C (HIV-1C) are needed, considering huge contribution HIV-1C to global epidemic.Plasma was obtained from 420 treatment-naïve infected Ethiopians recruited 2009-2011. The V3 region sequenced and coreceptor usage predicted by five tools: Geno2Pheno clinical-and clonal-models, PhenoSeq-C, C-PSSM Raymond's algorithm. impact...
Segment 8 mRNAs of influenza virus A/Brevig Misson/1918/1 (H1N1) are poorly spliced compared to segment A/Netherlands/178/95 (H3N2). Using oligonucleotide-mediated protein pull down with oligos spanning the entire length either H1N1 or H3N2 we identified cellular RNA binding proteins that interacted oligonucleotides derived from sequences. When hot spots for in were replaced by sequences, splicing efficiency increased significantly. Replacing as few three nucleotides mRNA sequences mRNA,...
Ebola virus glycoprotein (EBOV GP) is one of the most heavily O-glycosylated viral glycoproteins, yet we still lack a fundamental understanding structure its large mucin-like domain and to what degree host O-glycosylation capacity influences EBOV replication. Using tandem mass spectrometry, identified 47 O-glycosites on GP found similar glycosylation signatures virus-like particle- cell lysate-derived GP. Furthermore, performed quantitative differential O-glycoproteomics proteins produced in...
Animal models of hypertension are crucial for studying the pathophysiology diseases and developing new treatments. This study introduces a reproducible technique inducing surgical renovascular in rats using smaller renal artery clip. The groups were compared to control group standard drug assess effectiveness model its stability 21 days. results revealed progressive increase systolic diastolic blood pressure group. Moreover, heart rate body weight demonstrated sustained elevation at end...