A5040841157- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Mitochondrial Function and Pathology
- Probiotics and Fermented Foods
- Microbial Metabolites in Food Biotechnology
- Ubiquitin and proteasome pathways
- ATP Synthase and ATPases Research
- Bacterial Identification and Susceptibility Testing
- Protein Hydrolysis and Bioactive Peptides
- RNA and protein synthesis mechanisms
- Voice and Speech Disorders
- Hepatitis B Virus Studies
- Enterobacteriaceae and Cronobacter Research
- Nanoplatforms for cancer theranostics
- Advanced Proteomics Techniques and Applications
- Inflammatory Bowel Disease
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Advanced Nanomaterials in Catalysis
- Helicobacter pylori-related gastroenterology studies
- Viral gastroenteritis research and epidemiology
- Diverticular Disease and Complications
- DNA Repair Mechanisms
- Gastrointestinal motility and disorders
- Metal-Organic Frameworks: Synthesis and Applications
University of Pavol Jozef Šafárik
2016-2025
Bioscience (Slovakia)
2022-2025
Slovak Academy of Sciences
2012-2017
Institute of Molecular Biology of the Slovak Academy of Sciences
2012-2017
Czech Academy of Sciences, Institute of Experimental Medicine
2016
Fecal microbiota transplantation (FMT) seems to be a promising approach in ulcerative colitis (UC) management with the aim of repopulating patient's dysbiotic beneficial bacteria and restore its metabolic activity healthy characteristics. Metabolites present after FMT may improve function integrity intestinal barrier, reduce inflammation, thus induce remission an UC patient. In this study we evaluated whether Simulator Human Intestinal Microbial Ecosystem (SHIME®) model suitable non-invasive...
Mitochondrial nucleoids consist of several different groups proteins, many which are involved in essential cellular processes such as the replication, repair and transcription mitochondrial genome. The eukaryotic, ATP-dependent protease Lon is found within central nucleoid region, though little presently known about its role there. Aside from association with nucleoids, human also specifically interacts RNA. Recently, was shown to regulate TFAM, most abundant mtDNA structural factor...
Abstract Lon is an essential, multitasking AAA + protease regulating many cellular processes in species across all kingdoms of life. Altered expression levels the human mitochondrial ( h Lon) are linked to serious diseases including myopathies, paraplegia, and cancer. Here, we present first 3D structure full-length using cryo-electron microscopy. has a unique three-dimensional structure, which proteolytic ATP-binding domains (AP-domain) form hexameric chamber, while N-terminal domain...
The ability of probiotic strain Lactobacillus plantarum LS/07 to modify the activity intestinal bacterial enzymes - β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in prevention chronic diseases cancer, atherosclerosis dysbiosis was investigated. male Sprague-Dawley rats were randomly divided into 12 experimental groups: controls groups C (control), AT (atherosclerotic), CC (carcinogenic), dysbiotic each group combination with antibiotics (ATB), probiotics...
Lon, also called protease La, is an ATP-dependent present in all kingdoms of life. It involved protein quality control and several regulatory processes. Eukaryotic Lon possesses three domains, N-terminal domain, ATPase domain a proteolytic domain. requires ATP hydrolysis to digest larger, intact proteins, but can cleave small, fluorogenic peptides such as Glu-Ala-Ala-Phe-MNA by only binding, not hydrolyzing, ATP. Both peptidase activities be stimulated the binding larger substrate, β-casein....
The gut microbiome (GM) of rheumatic arthritis (RA) patients is often altered in composition and function. Moreover, methotrexate (MTX), one the most frequently used disease-modifying antirheumatic drugs, known to negatively affect GM composition. modulation immune system activity therapeutic benefits probiotics. aim current investigation was determine impact MTX therapy combined with Lactobacillus strains, Lactoplantibacillus plantarum LS/07 (LB), on adjuvant (AA) rats. Methods focused...
Dysbiosis of the gut microbiota, caused by antibiotics, plays a key role in establishment Clostridioides difficile CD). Toxin-producing strains are involved pathogenesis infection (CDI), one most common hospital-acquired infections. We cultured total 84 C. isolates from stool samples patients hospitalized at Louis Pasteur University Hospital Kosice, Slovakia, that were suspected CDI and further characterized molecular methods. The presence genes encoding toxin A, B, binary was assessed...
Abstract The effect of the probiotic strain Lactobacillus plantarum LS/07 on intestinal bacterial enzyme activities – β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in prevention cancer, atherosclerosis dysbiosis was investigated. Male Sprague-Dawley rats were randomly divided into 12 experimental groups: C (control group), AT (atherosclerotic CC (carcinogenic then each group combination with antibiotics probiotics individually double antibiotic probiotic. In...
Deviation in the gut microbial composition is involved various pathologies, including inflammatory bowel disease (IBD). Faecal microbiota transplant (FMT) can act as a promising approach to treat IBD by which changes microbiome be reversed and homeostasis restored. Therefore, aim of this study was investigate effect FMT on remission acute response using dextran sulfate sodium (DSS)-induced rat colitis model. communities were analysed 16S rRNA approach, clinical manifestations together with...
Objectives: The aim of this study was to confirm the effectiveness FMT on basis optimum composition faecal microbiota donor for support therapy in patients with ulcerative colitis, and observe viability frozen lyophilised administration forms under various storage conditions. Methods: bacterial samples assessed using amplicon sequencing via next-generation (NGS) technology, conducted Illumina MiSeq platform. BD FACS Canto flow cytometer used analyse metabolic activity samples. Results:...
Transplantation of faecal microbiota (FMT) is generally considered a safe therapeutic procedure with few adverse effects. The main factors that limit the spread use FMT therapy for idiopathic inflammatory bowel disease (IBD) are necessity minimising risk infection and transfer another disease. Obtaining animal model UC (ulcerative colitis) by exposure to DSS (dextran sodium sulphate) depends on many significantly affect result.
Background Clostridioides difficile infection (CDI) has become a serious health problem worldwide in recent years, the severity of which lies ability to spread epidemically hospitals and frequent diseases relapses. CDI Surveillance was performed at Louis Pasteur University Hospital Košice from January February 2020 analyse molecular characteristics ofC. difficile(CD) isolates local patients with CDI. Methods initially diagnosed using C. rapid test (for enzyme GDH toxin A/B). A total 36 stool...