A5073042046- Advanced Fluorescence Microscopy Techniques
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- DNA and Nucleic Acid Chemistry
- Lipid Membrane Structure and Behavior
- Genomics and Chromatin Dynamics
- Receptor Mechanisms and Signaling
- Photosynthetic Processes and Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Erythrocyte Function and Pathophysiology
- Ion channel regulation and function
- Immune Response and Inflammation
- Cell Image Analysis Techniques
- Advanced biosensing and bioanalysis techniques
- Retinoids in leukemia and cellular processes
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
- Estrogen and related hormone effects
- Advanced Biosensing Techniques and Applications
- Epigenetics and DNA Methylation
- Nanoplatforms for cancer theranostics
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
University of Debrecen
2016-2025
Max Planck Institute for Biophysical Chemistry
1996-2019
Discovery Institute
2015-2018
Sanford Burnham Prebys Medical Discovery Institute
2015-2018
Hungarian Academy of Sciences
2002-2011
University of Illinois Urbana-Champaign
2008
Inserm
2005-2006
Sorbonne Université
2005
Institut de Biologie Physico-Chimique
2005
Goethe University Frankfurt
2005
Transglutaminase 2 (TG2), a protein cross-linking enzyme with many additional biological functions, acts as coreceptor for integrin beta(3). We have previously shown that TG2(-/-) mice develop an age-dependent autoimmunity due to defective in vivo clearance of apoptotic cells. Here we report TG2 on the cell surface and guanine nucleotide-bound form promotes phagocytosis. Besides being binding partner beta(3), receptor known mediate uptake cells via activating Rac1, also show binds MFG-E8...
Immunogold staining and electron microscopy show that IL-2 receptor α-subunits exhibit nonrandom surface distribution on human T lymphoma cells. Analysis of interparticle distances reveals this clustering the scale a few hundred nanometers is independent presence expression IL-2R β-subunit. Clustering IL-2Rα confirmed by confocal microscopy, yielding same average cluster size, ≈600–800 nm, as microscopy. HLA class I II CD48 molecules also form clusters size. Disruption cholesterol-rich lipid...
Membrane proteins of cytotoxic T cells specifically reorganize to form an immunological synapse (IS) on interaction with their specific target. In this paper, we investigated the redistribution Kv1.3 channels, which are dominant voltage-gated potassium in plasma membrane allogen-activated human lymphocytes (CTLs) interacting target cells. channels bearing a FLAG epitope were expressed CTLs and cell-surface distribution fluorescently labeled ion was determined from confocal laser-scanning...
Fluorescence correlation microscopy (FCM) was applied to characterize fusion proteins of the green fluorescent protein (GFP) on cellular as well molecular level within seconds in an integrated instrument. FCM combines inherent sensitivity and high spatial resolution fluorescence spectroscopy with imaging micropositioning, thereby providing a spectrum information context. Signatures characteristic parameters derived from autocorrelation functions served distinguish GFP epidermal growth factor...
The private α-chains of IL-2 and IL-15 receptors (IL-2R IL-15R) share the signaling β- γ c -subunits, resulting in both common contrasting roles T cell function. Knowledge cytokine-dependent subunit assembly is indispensable for understanding paradox distinct capacities. By using fluorescence resonance energy transfer confocal microscopy, we have shown that IL-2Rα, IL-15Rα, IL-2/15Rβ as well MHC class I II glycoproteins formed supramolecular receptor clusters lipid rafts lymphoma line Kit...
The stoichiometry and composition of membrane protein receptors are critical to their function. However, the inability assess receptor subunit in situ has hampered efforts relate structures functional states. Here, we address this problem for asialoglycoprotein using ensemble FRET imaging, analytical modeling, single-molecule counting with photoactivated localization microscopy (PALM). We show that two subunits [rat hepatic lectin 1 (RHL1) RHL2] can assemble into both homo- hetero-oligomeric...
Abstract EGFP oligomers are convenient standards for experiments on fluorescent protein-tagged biomolecules. In this study, we characterized their hydrodynamic and fluorescence properties. Diffusion coefficients D of 1–4 were determined by analytical ultracentrifugation with detection correlation spectroscopy (FCS), yielding 83.4…48.2 μm 2 /s 97.3…54.8 from monomer to tetramer. A “barrels standing in a row” model agreed best the sedimentation data. Oligomerization red-shifted emission...
Retinoic acid receptors (RARs) and the vitamin D receptor (VDR) regulate distinct but overlapping gene sets in multiple cell types. The abundance characteristics of regulatory regions, occupied by both RARs VDR are largely unexplored. We used global approaches (ChIP-seq, RNA-seq, ATAC-seq) bioinformatics tools to map characterize common binding regions RARα differentiated human THP-1 cells. found that cistromes ligand-activated overlapped, their agonists (AM580 calcitriol) co-regulated...
Subunits (α, β and γ) of the interleukin‐2 receptor complex (IL‐2R) are involved in both proliferative activation‐induced cell death (AICD) signaling T cells. In addition, γ chains shared by other cytokines (e.g. IL‐7, IL‐9, IL‐15). However, molecular mechanisms responsible for recruiting/sorting α to at surface not clear. Here we show, four lines human adult lymphoma/leukemia origin, that three IL‐2R subunits compartmented together with HLA glycoproteins CD48 molecules plasma membrane,...
Blood coagulation factor XIII (FXIII) is a zymogen that transformed into an active transglutaminase by thrombin and Ca(2+). FXIII plays essential role in fibrin stabilization the protection of from proteolytic degradation. No convenient method has been available for measurement activity plasma. The aim present study was to improve optimize kinetic photometric assay originally developed our laboratory.In assay, activated Fibrin polymerization prevented inhibitory tetrapeptide. Glycine-ethyl...
Distribution and lateral organization of Kv1.3 potassium channels CD3 molecules were studied by using electron microscopy, confocal laser scanning fluorescence resonance energy transfer. Immunogold labeling microscopy showed that the distribution FLAG epitope-tagged (Kv1.3/FLAG) significantly differs from stochastic Poisson in plasma membrane human T lymphoma cells. Confocal images Kv1.3/FLAG accumulated largely overlapping areas. The numerical analysis crosscorrelation spatial intensity...
Interleukin 2 and interleukin 15 (IL2 IL15, respectively) provide quite distinct contributions to T-cell-mediated immunity, despite having similar receptor composition signaling machinery. As most of the proposed mechanisms underlying this apparent paradox attribute key significance individual α-chains IL2 IL15 receptors, we investigated spatial organization receptors IL2Rα IL15Rα at nanometer scale expressed on a human CD4+ leukemia T cell line using single-molecule-sensitive near-field...
With the evolving technology in CMOS integration, new classes of 2D-imaging detectors have recently become available. In particular, single photon avalanche diode (SPAD) arrays allow detection photons at high acquisition rates (\geq 100 kfps), which is about two orders magnitude higher than with currently available cameras. Here we demonstrate use a SPAD array for imaging fluorescence correlation spectroscopy (imFCS), tool to create 2D maps dynamics fluorescent molecules inside living cells....
The retinoic acid receptor (RAR) is a member of the nuclear superfamily. This ligand-inducible transcription factor binds to DNA as heterodimer with retinoid X (RXR) in nucleus. nucleus dynamic compartment and live-cell imaging techniques make it possible investigate action real-time. We studied diffusion EGFP–RAR by fluorescence correlation spectroscopy (FCS) uncover molecular interactions determining mobility. In absence ligand, we identified two distinct species different mobilities. fast...
The c-Fos and c-Jun transcription factors, members of the activator protein 1 (AP-1) complex, form heterodimers bind to DNA via a basic leucine zipper regulate cell cycle, apoptosis, differentiation, etc. Purified fragments could also stable homodimers, whereas homodimers were found be much less in earlier vitro studies. importance overexpression tumors controversy literature concerning homodimerization prompted us investigate Fos homodimerization. Förster resonance energy transfer (FRET)...
Aging contributes to cellular stress and neurodegeneration. Our understanding is limited regarding the tissue-restricted mechanisms providing protection in postmitotic cells throughout life. Here, we show that spinal cord motoneurons exhibit a high abundance of asymmetric dimethyl arginines (ADMAs) presence this posttranslational modification provides against environmental stress. We identify protein arginine methyltransferase 8 (PRMT8) as enzyme responsible for proper ADMA level neurons....
Retinoid X receptor (RXR) plays a pivotal role as transcriptional regulator and serves an obligatory heterodimerization partner for at least 20 other nuclear receptors (NRs). Given potentially limiting/sequestered pool of RXR simultaneous expression several partners, we hypothesized that NRs compete binding to this competition is directed by specific agonist treatment. Here, tested hypothesis on three NRs: peroxisome proliferator-activated gamma (PPARγ), vitamin D (VDR), retinoic acid alpha...