A5015060881- Endometrial and Cervical Cancer Treatments
- Ovarian cancer diagnosis and treatment
- Advanced Fluorescence Microscopy Techniques
- Cervical Cancer and HPV Research
- Gynecological conditions and treatments
- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Uterine Myomas and Treatments
- Endometriosis Research and Treatment
- Cancer Research and Treatments
- Colorectal and Anal Carcinomas
- Cancer Cells and Metastasis
- Nuclear Structure and Function
- Diversity and Career in Medicine
- Bacteriophages and microbial interactions
- Reproductive System and Pregnancy
- Microtubule and mitosis dynamics
- Minimally Invasive Surgical Techniques
- Gestational Trophoblastic Disease Studies
- Single-cell and spatial transcriptomics
- Force Microscopy Techniques and Applications
- Sarcoma Diagnosis and Treatment
- Surgical Simulation and Training
- Enzyme Structure and Function
- Lipid Membrane Structure and Behavior
Stanford University
2017-2024
Charité - Universitätsmedizin Berlin
2024
Gynecologic Oncology Group
2019-2024
Heinrich Heine University Düsseldorf
2007-2024
Stanford Cancer Institute
2022-2023
Albert Einstein College of Medicine
2010-2020
Montefiore Medical Center
2015-2020
Duke University
2020
Northwell Health
2020
Marine Biological Laboratory
2018
Rapidly emerging techniques of super-resolution single-molecule microscopy living cells rely on the continued development genetically encoded photoactivatable fluorescent proteins. On basis monomeric TagRFP, we have developed a TagRFP protein that is initially dark but becomes red after violet light irradiation. Compared to other dark-to-red proteins including PAmCherry, PATagRFP has substantially higher molecular brightness, better pH stability, less sensitivity blue light, and...
The stoichiometry and composition of membrane protein receptors are critical to their function. However, the inability assess receptor subunit in situ has hampered efforts relate structures functional states. Here, we address this problem for asialoglycoprotein using ensemble FRET imaging, analytical modeling, single-molecule counting with photoactivated localization microscopy (PALM). We show that two subunits [rat hepatic lectin 1 (RHL1) RHL2] can assemble into both homo- hetero-oligomeric...
The view on nuclear filaments formed by non-skeletal β-actin has significantly changed over the decades. Initially, filamentous actin was observed in amphibian oocyte nuclei and only under specific cell stress conditions mammalian nuclei. Improved labeling imaging technologies have permitted insights into a transient but microscopically apparent filament network that is relevant for chromatin organization, biomechanics of nucleus, gene expression, DNA damage repair. Here, we will provide...
Abstract The CapG protein, a Gelsolin‐related actin‐binding is expressed at higher levels in breast cancer, especially metastasizing than normal epithelium. Furthermore, it known that an increased expression of the protein triggers increase cell motility. According to vitro experiments, was supposed nuclear fraction which causes Here, we examined dynamical distribution within living cell, i.e . import into nucleus. kinetics invasive, cancer cells were compared non‐neoplastic similar FRAP...
This case series discusses a human papilloma virus (HPV)–related neovaginal squamous cell carcinoma in transgender woman and the need for formal gynecologic screening recommendations.
Despite the fundamental importance of diffusion for embryonic morphogen gradient formation in early Drosophila melanogaster embryo, there remains controversy regarding both extent and rate well-characterized morphogens. Furthermore, recent observation diffusional "compartmentalization" has suggested that may fact be nonideal mediated by an as-yet-unidentified mechanism. Here, we characterize effects geometry syncytial embryo on effective diffusivity cytoplasmic proteins. Our results...
In Brief OBJECTIVE: To evaluate the performance of simultaneous endometrial aspiration at time sonohysterography for screening postmenopausal women risk cancer. METHODS: A retrospective cohort study older than 50 years who underwent saline-infusion evaluation their endometrium. On completion imaging, remaining intracavitary saline and tissue were aspirated through catheter submitted pathologic evaluation. Based on clinical, pathologic, ultrasonographic results, patients surgical treatment...
Advanced mixed epithelial endometrial carcinomas are rare high-grade cancers with a poor prognosis. A clear cell component infers relative chemotherapy insensitivity, likely further increased by p53 wild type status and MMR deficiency. PD-1 inhibition for deficient has been recently added to first-line adjuvant treatment in combination platinum-based chemotherapy. Information on de-escalation of alone without is sparse.
Photoactivatable fluorescent proteins (PA-FPs) have been widely used to assess the dynamics of cell biological processes. In addition, PA-FPs enabled single-molecule based super-resolution imaging (photoactivated localization microscopy) and thereby provided unprecedented structural insight. For lack tools, however, fraction that is, actually being switched on fluoresce, photoactivation efficiency, has difficult assess. Uncertainty about efficiency hampered an understanding absolute amount...
ABSTRACT Objective To assess the long‐term outcome of postmenopausal women diagnosed with non‐atypical endometrial hyperplasia (NEH). Methods This was a retrospective study aged 55 or older who underwent sampling in our academic medical center between 1997 and 2008. Women had current recent (< 2 years) histological diagnosis NEH were included group compared those atrophic endometrium (AE). Outcome data obtained until February 2018. The main outcomes risk progression to carcinoma...
Objective Vulvar cancers account for 5% of all gynecologic malignancies; only 1%–3% those vulvar are primary sarcomas. Given the rarity sarcomas, outcome data specific to histopathologic subtypes sparse. The aim this study was identify clinical and pathologic factors sarcomas that associated with survival may inform treatment decisions. Methods Surveillance, Epidemiology, End Results (SEER) database searched women diagnosed sarcoma between 1973 2018. We identified 315 patients reviewed their...