A5082542719- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Dermatology and Skin Diseases
- Molecular Biology Techniques and Applications
- Immune cells in cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Acne and Rosacea Treatments and Effects
- Cancer-related molecular mechanisms research
- Peroxisome Proliferator-Activated Receptors
- Epigenetics and DNA Methylation
- Rheumatoid Arthritis Research and Therapies
- RNA Research and Splicing
- Hypothalamic control of reproductive hormones
- Cytokine Signaling Pathways and Interactions
- melanin and skin pigmentation
- Insect and Arachnid Ecology and Behavior
- Osteoarthritis Treatment and Mechanisms
- Psoriasis: Treatment and Pathogenesis
- Systemic Lupus Erythematosus Research
- Single-cell and spatial transcriptomics
- Retinoids in leukemia and cellular processes
- Immune Cell Function and Interaction
- Neonatal Respiratory Health Research
- Cardiovascular Disease and Adiposity
- Chronic Lymphocytic Leukemia Research
University of Debrecen
2016-2025
University of Nottingham
2020
Peking University
2019
UD-GenoMed (Hungary)
2012-2019
Peking University People's Hospital
2019
Hungarian Academy of Sciences
2012
University of Szeged
2010
Peroxisome proliferator-activated receptor γ (PPARγ) is a lipid-activated transcription factor regulating lipid metabolism and inflammatory response in macrophages dendritic cells (DCs). These immune exposed to distinct milieu show cell type specification as result of altered gene expression. We demonstrate here mechanism how molecules modulate PPARγ signaling subsets cells. Proinflammatory inhibited whereas interleukin-4 (IL-4) stimulated activity DCs. Furthermore, IL-4 augmented through an...
The molecular basis of signal-dependent transcriptional activation has been extensively studied in macrophage polarization, but our understanding remains limited regarding the determinants repression. Here we show that IL-4-activated STAT6 transcription factor is required for direct repression a large number genes during vitro and vivo alternative polarization. Repression results decreased lineage-determining factor, p300, RNA polymerase II binding followed by reduced enhancer expression,...
Abstract Macrophage gene expression determines phagocyte responses and effector functions. plasticity has been mainly addressed in vitro models that do not account for the environmental complexity observed vivo. In this study, we show microarray profiling revealed a highly dynamic landscape of transcriptomic changes Ly6CposCX3CR1lo Ly6CnegCX3CR1hi macrophage populations during skeletal muscle regeneration after sterile damage. Systematic analysis time elapsed, much more than Ly6C status, was...
While hypothalamic kisspeptin (KP) neurons play well-established roles in the estrogen-dependent regulation of reproduction, little is known about extrahypothalamic KP-producing (KP
Abstract Activation of vitamin D receptor (VDR) by 1,25-dihydroxyvitamin D3 (1,25-vitD) reprograms dendritic cells (DC) to become tolerogenic. Previous studies suggested that 1,25-vitD could inhibit the changes brought about differentiation and maturation DCs. Underpinning described phenotypic functional alterations, there must be 1,25-vitD-coordinated transcriptional events. However, this program has not been systematically investigated, particularly in a developmental context. Hence, it...
Abstract There are several open questions regarding the origin, development, and differentiation of subpopulations monocytes, macrophages (MFs), dendritic cells. It is a particularly intriguing question how circulating monocyte subsets develop contribute to generation steady-state inflammatory tissue MF pools which transcriptional mechanisms these processes. In this study, we took advantage genetic model in LyC6− development severely diminished due lack nuclear receptor, NUR77. We show that,...
The discovery of microRNAs (miRNAs) and their critical role in genetic control opened new avenues understanding various biological processes including immune cell lineage commitment, differentiation, proliferation apoptosis. However, a given miRNA may have hundreds different mRNA targets target might be regulated by multiple miRNAs, thus the characterisation dysregulated expression profiles could give better insight into development immunological disturbances autoimmune diseases. aim our...
Prior exposure to microenvironmental signals could fundamentally change the response of macrophages subsequent stimuli. It is believed that T helper-2 (Th2)-cell-type cytokine interleukin-4 (IL-4) and Toll-like receptor (TLR) ligand-activated transcriptional programs mutually antagonize each other, no remarkable convergence has been identified between them. In contrast, here, we show IL-4-polarized established a hyperinflammatory gene expression program upon lipopolysaccharide (LPS)...
RXR signaling is predicted to have a major impact in macrophages, but neither the biological consequence nor genomic basis of its ligand activation known. Comprehensive genome-wide studies were carried out map liganded RXR-mediated transcriptional changes, active binding sites, and cistromic interactions context macrophage genome architecture. The cistrome has 5200 which are not impacted by ligand. Active enhancers characterized PU.1 binding, an increase enhancer RNA, P300 recruitment. Using...
Summary Megakaryocyte (MK)-derived miRNAs have been detected in platelets. Here, we analysed the expression of platelet and circulating miR-223, miR-26b, miR-126 miR-140 that might be altered with their target mRNAs type 2 diabetes mellitus (DM2). MiRNAs were isolated from leukocyte-depleted platelets plasma samples obtained 28 obese DM2, 19 non-DM 23 healthy individuals. The effect hyperglycaemia on was also evaluated MKs using MEG-01 K562 cells under hyperglycaemic conditions after 8 hours...
In addition to producing sebum, sebocytes link lipid metabolism with inflammation at a cellular level and hence, greatly resemble adipocytes. However, so far no analysis was performed identify characterize the adipocyte-associated inflammatory proteins, members of adipokine family in sebocytes. Therefore, we determined expression profile adipokines [adiponectin, interleukin (IL) 6, resistin, leptin, serpin E1, visfatin, apelin, chemerin, retinol-binding protein 4 (RBP4) monocyte...
Serotonin is a monoamine neurotransmitter that signals through wide array of receptors (5-HT1-7) many which are also involved in immune processes. Dendritic cells (DCs) crucial players defense by bridging innate and adaptive responses via their vast repertoire pattern recognition antigen-presenting capability. Although serotonin known to influence immunity at levels, cell type-specific expression function its remains poorly understood. Here we aimed study 5-HT1-7 CD1a- CD1a+ human...
Rosacea is a common chronic inflammation of sebaceous gland-rich facial skin characterized by severe dryness, elevated pH, transepidermal water loss, and decreased hydration levels. Until now, there has been no thorough molecular analysis permeability barrier alterations in the patients with rosacea. Thus, we aimed to investigate papulopustular rosacea samples compared healthy skin, using RNA sequencing (n = 8). Pathway analyses Cytoscape ClueGO revealed 15 significantly enriched pathways...
The disease-residual transcriptomic profile (DRTP) within psoriatic healed/resolved skin and epidermal tissue-resident memory T (TRM) cells have been proposed to be crucial for the recurrence of old lesions. However, it is unclear whether keratinocytes are involved in disease recurrence. There increasing evidence regarding importance epigenetic mechanisms pathogenesis psoriasis. Nonetheless, changes that contribute psoriasis remain unknown. aim this study was elucidate role relapse. marks...
Retinoid X receptors (RXRs) are heterodimerization partners for many nuclear and also act as homodimers. Heterodimers formed by RXR a nonpermissive partner, e.g. retinoic acid receptor (RAR) vitamin D (VDR), can be activated only the agonist of partner receptor. In contrast, heterodimers that contain permissive partners, liver (LXR) peroxisome proliferator-activated (PPAR), agonists either or RXR, raising possibility pleiotropic signaling. However, it is not known to what extent receptor's...
Abstract Dendritic cells (DCs) respond to changes in their lipid environment by altering gene expression and immunophenotype. Some of these alterations are mediated via the nuclear receptor superfamily. However, little is known about contribution liver X (LXR) DC biology. In this study, we present a systematic analysis LXR, activated synthetic ligands or naturally occurring oxysterols developing human monocyte-derived DCs. We found that LXRs can be throughout differentiation monocyte-...
Retinoid X receptor (RXR) is an obligate heterodimeric partner of several nuclear receptors (NRs), and as such a central component NR signaling regulating the immune metabolic phenotype macrophages. Importantly, binding motifs RXR heterodimers are enriched in tissue-selective open chromatin regions resident macrophages, suggesting roles subtype specification. Recent genome-wide studies revealed that binds to thousands sites genome, but mechanistic details how cistrome established serves...
Abstract Corneal tissue regeneration is of crucial importance for maintaining normal vision. We aimed to isolate and cultivate human corneal stroma-derived mesenchymal stem-like cells (CSMSCs) from the central part cadaver corneas study their phenotype, multipotency, role in immunity wound healing. The isolated grew as monolayers vitro , expressed mesenchymal- stemness-related surface markers (CD73, CD90, CD105, CD140b) were negative hematopoietic determined by flow cytometry. CSMSCs able...
Macrophages polarize into functionally distinct subtypes while responding to microenvironmental cues. The identity of proximal transcription factors (TFs) downstream from the polarization signals are known, but their activity is typically transient, failing explain long-term, stable epigenomic programs developed. Here, we mapped early and late changes interleukin-4 (IL-4)-induced alternative macrophage polarization. We identified TF, growth response 2 (EGR2), bridging transient gene...
In sepsis, platelets may become activated via toll-like receptors (TLRs), causing microvascular thrombosis. Megakaryocytes (MKs) also express these receptors; thus, severe infection modulate thrombopoiesis. To explore the relevance of altered miRNAs in platelet activation upon we first investigated sepsis-induced miRNA expression septic patients. The effect abnormal Dicer level on was evaluated. were profiled vs. normal using TaqMan Open Array. We validated miR-26b with its target SELP...