A5009528433- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- T-cell and B-cell Immunology
- Psoriasis: Treatment and Pathogenesis
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Biotin and Related Studies
- Dermatology and Skin Diseases
- Bioinformatics and Genomic Networks
- Skin and Cellular Biology Research
- Biochemical Analysis and Sensing Techniques
- Antibiotic Resistance in Bacteria
- Adipose Tissue and Metabolism
- melanin and skin pigmentation
- Advanced Proteomics Techniques and Applications
- Autoimmune Bullous Skin Diseases
- Sleep and Wakefulness Research
- Computational Drug Discovery Methods
- Asthma and respiratory diseases
- Regulation of Appetite and Obesity
- Cancer Genomics and Diagnostics
- Evolution and Genetic Dynamics
- Vaccine Coverage and Hesitancy
- Renal Transplantation Outcomes and Treatments
HUN-REN Szegedi Biológiai Kutatóközpont
2018-2025
Institute of Biochemistry
2018-2025
University of Szeged
2015-2024
Research Network (United States)
2023
Hungarian Research Network
2021-2022
Hungarian Academy of Sciences
2018-2021
MTA-SZTE Research Group on Artificial Intelligence
2015
Despite ongoing antibiotic development, evolution of resistance may render candidate antibiotics ineffective. Here we studied in vitro emergence to 13 introduced after 2017 or currently compared with in-use antibiotics. Laboratory showed that clinically relevant arises within 60 days exposure Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa, priority Gram-negative ESKAPE pathogens. Resistance mutations are already present natural populations...
Central players of the adaptive immune system are groups proteins encoded in major histocompatibility complex (MHC), which shape response against pathogens and tolerance to self-peptides. The corresponding genomic region is particular interest, as it harbors more disease associations than any other human genome, including with infectious diseases, autoimmune disorders, cancers, neuropsychiatric diseases. Certain MHC molecules can bind a much wider range epitopes others, but functional...
The disease-residual transcriptomic profile (DRTP) within psoriatic healed/resolved skin and epidermal tissue-resident memory T (TRM) cells have been proposed to be crucial for the recurrence of old lesions. However, it is unclear whether keratinocytes are involved in disease recurrence. There increasing evidence regarding importance epigenetic mechanisms pathogenesis psoriasis. Nonetheless, changes that contribute psoriasis remain unknown. aim this study was elucidate role relapse. marks...
Data indicate that in psoriasis, abnormalities are already present nonlesional skin. Transforming growth factor-β and keratinocyte factor (KGF), together with fibronectin α5β1 integrin, were suggested to play a crucial role the pathogenesis of psoriasis by influencing inflammation hyperproliferation.To investigate expression KGF, fibroblast receptor (FGFR)2, (FN) extra domain A (EDA)-positive FN healthy psoriatic skin, study effect KGF on regulation EDA(+) production fibroblasts.Healthy,...
Introduction Tobacco smoking generates airway inflammation in chronic obstructive pulmonary disease (COPD), and its involvement the development of lung cancer is still among leading causes early death. Therefore, we aimed to have a better understanding disbalance immunoregulation inflammatory conditions smoker subjects with stable COPD (stCOPD), exacerbating (exCOPD), or non-small cell (NSCLC). Methods Smoker controls without illness were recruited as controls. Through extensive mapping...
Psoriasis is a multifactorial inflammatory skin disease characterized by increased proliferation of keratinocytes, activation immune cells and susceptibility to metabolic syndrome. Systems biology approach makes it possible reveal novel important factors in the pathogenesis disease. Protein-protein, protein-DNA, merged (containing both protein-protein protein-DNA interactions) chemical-protein interaction networks were constructed consisting differentially expressed genes (DEG) between...
Abstract To better understand the pathomechanism of psoriasis, a comparative proteomic analysis was performed with non-lesional and lesional skin from psoriasis patients healthy individuals. Strikingly, 79.9% proteins that were differentially expressed in exhibited expression levels within twofold observed skin, suggesting represents an intermediate stage. Proteins outside this trend categorized into three groups: I. exhibiting similar to which might be predisposing factors (i.e., CSE1L,...
Precise spatiotemporal protein organization is critical for fundamental biological processes including cell division. Indeed, aberrant mitosis and mitotic factors are involved in diverse diseases, various cancers, Alzheimer's disease, rare diseases. During mitosis, complex spatial rearrangements regulation ensure the accurate separation of replicated sister chromatids to produce genetically identical daughter cells. Previous studies employed high-throughput methodologies follow specific...
The identification of immunogenic peptides has become essential in an increasing number fields immunology, ranging from tumor immunotherapy to vaccine development. nature the adaptive immune response is shaped by similarity between foreign and self-protein sequences, a concept extensively applied numerous studies. Can we precisely define degree self? Furthermore, do accurately In current work, aim unravel conceptual mechanistic vagueness hindering assessment self-similarity. Accordingly,...
We found that Sweet potato feathery mottle virus (SPFMV) P1, a close homologue of mild did not have any silencing suppressor activity. Remodeling the Argonaute (AGO) binding domain SPFMV P1 by introduction two additional WG/GW motifs converted it to with AGO capacity. To our knowledge, this is first instance transformation viral protein unknown function functional suppressor.
Abstract We previously described a novel in vitro culture technique for dedifferentiated human adult skin melanocytes. Melanocytes cultured defined, cholera toxin and PMA free medium became bipolar, unpigmented, highly proliferative. Furthermore, TRP‐1 c‐Kit expression disappeared EGFR receptor nestin were induced the cells. Here, we further characterized phenotype of these cells by comparing them to mature pigmented melanocytes detected crucial steps their change. Our data suggest that...
HLA class II proteins are important elements of human adaptive immune recognition and associated with numerous infectious immune-mediated diseases. These highly variable molecules can be classified into DP, DQ DR groups. It has been proposed that in contrast DP DR, epitope binding by variants rather results tolerance. However, the pieces evidence limited controversial. We found bind more epitopes than DR. Pathogen-associated bound similar to ones Accordingly, significantly different pathogen...
Adaptive immune recognition is mediated by the binding of peptide-human leukocyte antigen complexes T cells. Positive selection cells in thymus a fundamental step generation responding cell repertoire: only those survive that recognize human peptides presented on surface cortical thymic epithelial We propose while this essential for optimal function, process results defective repertoire because it self-peptides. To test our hypothesis, we focused amino acid motifs contact with receptors....
As drug development is extremely expensive, the identification of novel indications for in-market drugs financially attractive. Multiple algorithms are used to support such repurposing, but highly reliable methods combining simulation intracellular networks and machine learning currently not available. We developed an algorithm that simulates effects on flow information through protein-protein interaction networks, vector identify potentially effective in our model disease, psoriasis. Using...
Abstract Despite the ongoing development of new antibiotics, future evolution bacterial resistance may render them ineffective. We demonstrate that antibiotic candidates currently under are as prone to in Gram-negative pathogens clinically employed antibiotics. Resistance generally stems from both genomic mutations and transfer genes microbiomes associated with humans, factors carrying equal significance. The molecular mechanisms overlap those found commonly used Therefore, these already...
Keratinocytes are one of the primary cells affected by psoriasis inflammation. Our study aimed to delve deeper into their morphology, transcriptome, and epigenome changes in response psoriasis-like We created a novel cytokine mixture mimic mild severe inflammatory conditions cultured keratinocytes. Upon induction inflammation, we observed that keratinocytes exhibited mesenchymal-like phenotype, further confirmed increased VIM mRNA expression results obtained from confocal microscopy....
To better understand the molecular events underlying vulvovaginal candidiasis, we established an in vitro system. Immortalized vaginal epithelial cells were infected with live, yeast form C. albicans and cultured same medium without used as control. In both cases a to hyphae transition was robustly induced. Whole transcriptome sequencing identify specific gene expression changes . Numerous genes leading upregulated control response cells. Strikingly, GlcNAc pathway exclusively triggered by...
The diagnosis of severe dermatological infections is usually not the pathologist’s task, but sometimes pathological enables beginning targeted or even lifesaving therapy. An infectious disease considered unusual when it rare in given climate causative agent normally cause human illness, itself very rare. In case an uncommon with generalized skin symptoms and possibility origin biopsy easily feasible, relatively quick precise „bed-side” histological could be lifesaving. Authors present such...