A5085974441- Hypothalamic control of reproductive hormones
- Regulation of Appetite and Obesity
- Neuroendocrine regulation and behavior
- Receptor Mechanisms and Signaling
- Ovarian function and disorders
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Biochemical Analysis and Sensing Techniques
- Estrogen and related hormone effects
- Stress Responses and Cortisol
- Lipid Membrane Structure and Behavior
- Sleep and Wakefulness Research
- Plant Reproductive Biology
- Complement system in diseases
- Ion channel regulation and function
- Adipose Tissue and Metabolism
- Memory and Neural Mechanisms
- Cannabis and Cannabinoid Research
- Photoreceptor and optogenetics research
- RNA Research and Splicing
- Neuropeptides and Animal Physiology
- stochastic dynamics and bifurcation
- Neurobiology and Insect Physiology Research
- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
Czech Academy of Sciences, Institute of Experimental Medicine
2021-2025
HUN-REN Institute of Experimental Medicine
2011-2024
Hungarian Research Network
2022-2024
Hungarian Academy of Sciences
2009-2020
National Food Chain Safety Office
2012
Nagoya City University
1998-2008
Molecular Biology Consortium
2003
University of Szeged
1988-2002
Mizuho (Japan)
1999
IntroductionGhrelin and cannabinoids stimulate appetite, this effect possibly being mediated by the activation of hypothalamic AMP-activated protein kinase (AMPK), a key enzyme in appetite metabolism regulation. The cannabinoid receptor type 1 (CB1) antagonist rimonabant can block orexigenic ghrelin. In study, we have elucidated mechanism putative ghrelin-cannabinoid interaction.MethodsThe effects ghrelin CB1 wild-type mice, CB1-knockout animals, were studied on food intake, AMPK activity...
Here, we sought to demonstrate that the orexigenic circulating hormone, ghrelin, is able exert neurobiological effects (including those linked feeding control) at level of amygdala, involving neuroanatomical, electrophysiological and behavioural studies. We found ghrelin receptors (GHS-R) are densely expressed in several subnuclei notably ventrolateral (LaVL) ventromedial (LaVM) parts lateral amygdaloid nucleus. Using whole-cell patch clamp electrophysiology record from cells nucleus,...
While hypothalamic kisspeptin (KP) neurons play well-established roles in the estrogen-dependent regulation of reproduction, little is known about extrahypothalamic KP-producing (KP
Cannabinoids suppress fertility via reducing hypothalamic GnRH output. γ-Aminobutyric acid (GABA)A receptor (GABAA-R)-mediated transmission is a major input to cells that can be excitatory. We hypothesized cannabinoids act inhibiting GABAergic input. performed loose-patch electrophysiological studies of acute slices from adult male GnRH-green fluorescent protein transgenic mice. Bath application type 1 cannabinoid (CB1) agonist WIN55,212 decreased neuron firing rate. This action was...
The parabrachial nucleus (PBN) is a key for the regulation of feeding behavior. Inhibitory inputs from hypothalamus to PBN play crucial role in normal maintenance behavior, because their loss leads starvation. Viscerosensory stimuli result neuronal activation PBN. However, origin and neurochemical identity excitatory input remain largely unexplored. Here, we hypothesize that hindbrain glucagon-like peptide 1 (GLP-1) neurons provide PBN, which may lead reduction Our data, obtained mice...
An important question in behavioral neurobiology is how particular neuron populations and pathways mediate the overall roles of brain structures. Here we investigated this issue by studying medial prefrontal cortex (mPFC), an established locus inhibitory control aggression. We male rats that dominantly distinct mPFC project to produce dense fiber networks with glutamate release sites mediobasal hypothalamus (MBH) lateral (LH; i.e., two executory centers species-specific violent bites,...
Activation of the complement system on cell surface results in insertion pore forming membrane attack complexes (MAC, C5b‐9). In order to protect themselves from attack, cells express several regulatory molecules, including terminal complex regulator CD59 that inhibits assembly large MACs by inhibiting additional C9 molecules into C5b‐9 complex. Using whole patch clamp method, we were able measure accumulation homologous − human B‐cells, which formed non‐selective ion channels with a total...
The orexigenic peptide, ghrelin is known to influence function of GnRH neurons, however, the direct effects hormone upon these neurons have not been explored, yet. present study was undertaken reveal expression growth secretagogue receptor (GHS-R) in and elucidate mechanisms actions them. Ca2+-imaging revealed a ghrelin-triggered increase Ca2+-content GT1-7 kept steroid-free medium, which abolished by GHS-R-antagonist JMV2959 (10µM) suggesting action ghrelin. Estradiol (1nM) eliminated...
Glucagon-like peptide-1 (GLP-1), a metabolic signal molecule, regulates reproduction, although, the involved molecular mechanisms have not been elucidated, yet. Therefore, responsiveness of gonadotropin-releasing hormone (GnRH) neurons to GLP-1 analog Exendin-4 and elucidation pathways acting downstream receptor (GLP-1R) challenged. Loose patch-clamp recordings revealed that (100 nM-5 μM) elevated firing rate in hypothalamic GnRH-GFP male mice via activation GLP-1R. Whole-cell measurements...
1. We report the first experimental evidence of a neuronal C5a receptor (nC5aR) in human cells origin. Expression nC5aR mRNA was demonstrated by reverse transcriptase-polymerase chain reaction (RT-PCR) TGW neuroblastoma cells. 2. functional C5aR supported finding that evoked transient increase intracellular calcium level as measured flow cytometry (FACS). 3. To analyse function nC5aR, an antisense peptide fragment used. Previous data showed (a termed PR226) has agonist and antagonist effects...
Excessive fear learning and generalized, extinction-resistant memories are core symptoms of anxiety trauma-related disorders. Despite significant evidence from clinical studies reporting hyperactivity the bed nucleus stria terminalis (BNST) under these conditions, role BNST in expression is still not clarified. Here, we tested how modulates male mice using a chemogenetic approach. Activation GABAergic neurons during conditioning or memory consolidation resulted enhanced cue-related recall....
Kisspeptin neurons in the mediobasal hypothalamus (MBH) are critical targets of ovarian estrogen feedback regulating mammalian fertility. To reveal molecular mechanisms underlying this signaling, we thoroughly characterized estrogen-regulated transcriptome kisspeptin cells from ovariectomized transgenic mice substituted with 17β-estradiol or vehicle. MBH were harvested using laser-capture microdissection, pooled, and subjected to RNA sequencing. Estrogen treatment significantly ( p.adj ....
Abstract In our earlier results, we demonstrated that cells expressing the complement C5aR are vulnerable since abnormal activation of caused apoptosis these cells. this study, demonstrate by antisense homology box (AHB) peptides synthesized in multiple antigenic peptide form and representing putative interaction sites C5a/C5aR evoked calcium influx TGW neuroblastoma Dose-dependent inhibition response was found when were pretreated with C5a, suggesting involved process. addition,...
Human reproduction is controlled by ~2000 hypothalamic gonadotropin-releasing hormone (GnRH) neurons. Here, we report the discovery and characterization of additional ~150,000–200,000 GnRH-synthesizing cells in human basal ganglia forebrain. Nearly all extrahypothalamic GnRH neurons expressed cholinergic marker enzyme choline acetyltransferase. Similarly, were also both embryonic adult brains. Whole-transcriptome analysis interneurons medium spiny projection laser-microdissected from putamen...
GnRH-synthesizing neurons orchestrate reproduction centrally. Early studies have proposed the contribution of acetylcholine (ACh) to hypothalamic control reproduction, although causal mechanisms haven't been clarified. Here, we report that in vivo pharmacogenetic activation cholinergic system increased secretion luteinizing hormone (LH) orchidectomized mice. 3DISCO immunocytochemistry and electron microscopy revealed innervation GnRH by axons. Retrograde viral labeling initiated from...
Abstract Surge release of gonadotropin-releasing hormone (GnRH) is essential in the activation pituitary gonadal unit at proestrus afternoon preceded by rise serum 17β-estradiol (E2) level during positive feedback period. Here, we describe a mechanism estradiol regulation acting directly on GnRH-green fluorescent protein (GFP) neurons mice. Whole-cell clamp and loose patch recordings revealed that high physiological dose (200 pM), significantly increased firing rate afternoon. The mPSC...
In order to investigate haemotropic Mycoplasma (formerly Eperythrozoon) infection of goats, blood samples and blood-sucking lice (Linognathus stenopsis) were collected in two goat herds. DNA was extracted from 20 49 allocated six pools according host individuals. Haemoplasma detected four goats by real-time PCR. From the sample with highest bacterial load simultaneous presence M. ovis 'Candidatus haemoovis' demonstrated cloning sequencing. Louse haemoplasma negative, including those...
Gonadotropin-releasing hormone (GnRH) neurons are controlled by 17β-estradiol (E2) contributing to the steroid feedback regulation of reproductive axis. In rodents, E2 exerts a negative effect upon GnRH throughout estrus-diestrus phase ovarian cycle. The present study was undertaken reveal role estrogen receptor subtypes in mediation signal and elucidate downstream molecular machinery suppression. administration at low physiological concentration (10 pM) on acute brain slices obtained from...
Abstract PL37 (RAARISLGPRCIKAFTE) is an antisense homology box peptide composed of aa 37–53 C5a-anaphylatoxin and considered to be the region essential for C5a function. Using a computer program, we designed complementary peptides ASGAPAPGPAGPLRPMF (Pep-A) ASTAPARAGLPRLPKFF (Pep-B). Pep-A bound with very slow dissociation as determined by analysis using surface plasmon resonance, whereas Pep-B failed bind at all. was inactivated concentrations 7 nM or more Pep-A, this concentration inhibited...
Neuropeptide Y (NPY) is one of the most potent orexigenic peptides. The hypothalamic paraventricular nucleus (PVN) a major locus where NPY exerts its effects on energy homeostasis. We investigated how effect within PVN. Patch clamp electrophysiology and Ca2+ imaging were used to understand involvement signaling retrograde transmitter systems in mediation induced Immuno-electron microscopy performed elucidate subcellular localization elements nitric oxide (NO) system parvocellular In vivo...
In proestrus, the changing gonadal hormone milieu alters physiological properties of GnRH neurons and contributes to development surge. We hypothesized that proestrus also influences expression different ion channel genes in mouse neurons. Therefore, we performed gene profiling collected from intact, proestrous metestrous GnRH-GFP transgenic mice, respectively. Proestrus changed 37 8 calcium homeostasis-regulating genes. Voltage-gated sodium channels responded with upregulation three alpha...
Kisspeptin (KP) neurones in the rostral periventricular area of third ventricle (RP3V) and arcuate nucleus (Arc) are important elements neuronal circuitry regulating gonadotropin-releasing hormone (GnRH) secretion. KP co-synthesised neuropeptides/neurotransmitters act directly on GnRH perikarya processes. not only form final output pathway reproductive functions anterior pituitary gland, but also provide input to sites within hypothalamus. The current double-label immunohistochemical studies...