A5051186528- Drug Transport and Resistance Mechanisms
- Pluripotent Stem Cells Research
- Trace Elements in Health
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Cholesterol and Lipid Metabolism
- Pharmacological Effects and Toxicity Studies
- Adenosine and Purinergic Signaling
- HIV/AIDS drug development and treatment
- Pediatric Hepatobiliary Diseases and Treatments
- Tissue Engineering and Regenerative Medicine
- Hemoglobinopathies and Related Disorders
- Nanoparticle-Based Drug Delivery
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Gout, Hyperuricemia, Uric Acid
- Neuroinflammation and Neurodegeneration Mechanisms
- Amino Acid Enzymes and Metabolism
- Liver physiology and pathology
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Genomics and Rare Diseases
- Neuroscience and Neural Engineering
- Renal and related cancers
- Phagocytosis and Immune Regulation
HUN-REN Research Centre for Natural Sciences
2016-2025
Institute of Molecular Life Sciences
2015-2023
Hungarian Academy of Sciences
2009-2021
Hungarian Research Network
2020
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2014
National Institutes of Health
2014
Semmelweis University
2008-2011
University of North Carolina at Chapel Hill
1999-2000
Hungarian National Blood Transfusion Service
1991-1996
In this report we show that NIH-3T3 mouse fibroblasts stably expressing the human multidrug transporter (MDR1 or P-glycoprotein), in contrast to control cells, actively extrude hydrophobic acetoxymethyl ester (AM) derivatives used for cellular loading of various fluorescent calcium and pH indicators. This dye extrusion is blocked by competing substrates inhibitors transporters, e.g. verapamil, vincristine, sodium orthovanadate, oligomycin, a monoclonal anti-MDR1 antibody. The hydrophilic...
Airway epithelia are positioned at the interface between body and environment, generate complex signaling responses to inhaled toxins other stresses. Luminal mechanical stimulation of airway epithelial cells produces a propagating wave elevated intracellular Ca2+ that coordinates components integrated stress response. In polarized epithelia, this response has been attributed IP3 permeation through gap junctions. Using combination approaches, including enzymes destroy extracellular...
Abstract Iressa (ZD1839, Gefitinib), used in clinics to treat non–small cell lung cancer patients, is a tyrosine kinase receptor inhibitor that leads specific decoupling of epidermal growth factor (EGFR) signaling. Recent data indicate especially effective tumors with certain EGFR mutations; however, subset these does not respond Iressa. In addition, populations have an elevated risk side effects during treatment. The human ABCG2 (BCRP/MXR/ABCP) transporter causes drug resistance by actively...
In this paper we demonstrate that the expression of multidrug resistance‐associated protein (MRP) in a variety intact human tumour cells results ATP‐dependent, mutually exclusive extrusion both acetoxymethyl ester and free anion forms fluorescent dye calcein, as well pyrenemaleimide‐glutathione conjugate. The MRP‐dependent transport all these three model compounds closely correlates with level MRP is cross‐inhibited by hydrophobic anticancer drugs, reversing agents for MDR1, also not...
Extracellular nucleotides are believed to be important regulators of ion transport in epithelial tissues as a result their ability activate cell surface receptors. Although numerous receptors that bind have been identified, the complexity this receptor family, combined with lack pharmacological agents specific for these receptors, has made assignment particular and ligands physiological responses difficult. Because ATP UTP appear equipotent equieffective regulating many epithelia, we tested...
To test for the role of P2Y<sub>2</sub> receptor (P2Y<sub>2</sub>-R) in regulation nucleotide-promoted Ca<sup>2+</sup> signaling lung, we generated P2Y<sub>2</sub>-R-deficient (P2Y<sub>2</sub>-R(−/−)) mice and measured intracellular Ca<sup>2+</sup><sub>i</sub> responses (ΔCa<sup>2+</sup><sub>i</sub>) to nucleotides cultured lung fibroblasts nasal tracheal epithelial cells from wild type P2Y<sub>2</sub>-R(−/−) mice. In fibroblasts, rank order potencies nucleotide-induced...
Abstract Synaptic functional disturbances with concomitant synapse loss represent central pathological hallmarks of Alzheimer’s disease. Excessive accumulation cytotoxic amyloid oligomers is widely recognized as a key event that underlies neurodegeneration. Certain complement components are crucial instruments widespread because they can tag synapses impairments leading to their engulfment by microglia. However, an exact understanding the affected synaptic functions predispose...
The P2Y<sub>4</sub> receptor is selectively activated by UTP. Although addition of neither ATP nor UDP alone increased intracellular Ca<sup>2+</sup> in 1321N1 human astrocytoma cells stably expressing the receptor, combined these nucleotides resulted a slowly occurring elevation Ca<sup>2+</sup>. possibility that stimulatory effect reflected formation UTP an extracellular transphosphorylating activity was investigated. Incubation with [<sup>3</sup>H]UDP or [<sup>3</sup>H]ADP under conditions...
Abstract Dendritic cells (DCs) respond to changes in their lipid environment by altering gene expression and immunophenotype. Some of these alterations are mediated via the nuclear receptor superfamily. However, little is known about contribution liver X (LXR) DC biology. In this study, we present a systematic analysis LXR, activated synthetic ligands or naturally occurring oxysterols developing human monocyte-derived DCs. We found that LXRs can be throughout differentiation monocyte-...
Small extracellular vesicles (EVs) are membrane enclosed structures that usually released from cells upon exocytosis of multivesicular bodies (MVBs) as a collection separate, free EVs. In this study, we analysed paraffin embedded sections archived human colorectal cancer samples. We studied 3D reconstructions confocal microscopic images complemented by HyVolution and STED imaging. Unexpectedly, found evidence large, MVB-like aggregates ALIX/CD63 positive EV clusters were en bloc migrating...
In this report we demonstrate that various biologically active hydrophobic peptide derivatives, e.g., proteinase inhibitors, chemoattractants, ionophores, enkephalins, and immunosuppressants, stimulate a membrane ATPase activity associated with the human multidrug transporter (MDR1). The stimulation of MDR1-ATPase by these agents does not correlate their known biochemical or pharmacological activities but rather hydrophobicity. peptides show high-affinity interaction also interfere strongly...
Abstract Human embryonic stem (HuES) cells represent a new potential tool for cell-therapy and gene-therapy applications. However, these approaches require the development of efficient, stable gene delivery, proper progenitor cell tissue separation methods. In HuES lines, we have generated stable, enhanced green fluorescent protein (EGFP)-expressing clones using transposon-based (Sleeping Beauty) system. This method yielded high percentage transgene integration expression. Similarly to...