A5056336001- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer Genomics and Diagnostics
- Gastrointestinal disorders and treatments
- Circular RNAs in diseases
- Colorectal Cancer Treatments and Studies
- Drug Transport and Resistance Mechanisms
- Renal cell carcinoma treatment
- Gastrointestinal Tumor Research and Treatment
- interferon and immune responses
- Tracheal and airway disorders
- Soft tissue tumors and treatment
- Multiple and Secondary Primary Cancers
- Tumors and Oncological Cases
- Abdominal vascular conditions and treatments
- IgG4-Related and Inflammatory Diseases
- Genetic and Kidney Cyst Diseases
- Biliary and Gastrointestinal Fistulas
- Liver physiology and pathology
- Caveolin-1 and cellular processes
- Neuroendocrine Tumor Research Advances
- Vascular Tumors and Angiosarcomas
Uzsoki Hospital
2011-2023
Semmelweis University
2021
Memorial Hospital of South Bend
2010-2013
Extracellular vesicles (EVs) are membrane-surrounded structures that transmit biologically important molecules from the releasing to target cells, thus providing a novel intercellular communication mechanism. Since EVs carry their cargo in protected form and secretion is generally increased tumorigenesis, hold great potential for early cancer diagnosis. By 3D culturing, we provide evidence colorectal (CRC) patient-derived organoids, representing state-of-the-art established essential...
The aim of the present study was to analyse preoperative platelet count and platelet-lymphocyte ratio (PLR) in patients with colorectal cancer (CRC) different stages hepatic metastasis CRC (mCRC) compare these factors as potential prognostic markers. Clinicopathological data 10 years were collected retrospectively from 336 118 mCRC. Both mCRC group overall survival (OS) significantly worse who had elevated (hazard [HR] = 2.2, p < 0.001 HR 2.9, 0.018, respectively). Multivariate analysis...
Small extracellular vesicles (EVs) are membrane enclosed structures that usually released from cells upon exocytosis of multivesicular bodies (MVBs) as a collection separate, free EVs. In this study, we analysed paraffin embedded sections archived human colorectal cancer samples. We studied 3D reconstructions confocal microscopic images complemented by HyVolution and STED imaging. Unexpectedly, found evidence large, MVB-like aggregates ALIX/CD63 positive EV clusters were en bloc migrating...
Abstract Extracellular vesicles (EV) are membrane-surrounded that represent a novel way of intercellular communication by carrying biologically important molecules in concentrated and protected form. The intestinal epithelium is continuously renewed small proliferating stem cell (ISC) population, residing at the bottom crypts specific microenvironment, niche. By using 3D mouse human organoids, we show fibroblast-derived EVs involved forming ISC niche transmitting Wnt epidermal growth factor...
Extracellular vesicles (EV), structures surrounded by a biological membrane, transport biologically active molecules, and represent recently identified way of intercellular communication. Colorectal cancer (CRC), one the most common types in Western countries, is composed both tumor stromal cells amount fibroblasts negatively correlates with patient survival. Here we show that normal colon (NCF) release EVs characteristic miRNA cargo profile when stimulated TGFβ, important activating factors...
Abstract The majority of colorectal cancer (CRC) patients carry mutations in the APC gene, which lead to unregulated activation Wnt pathway. Extracellular vesicles (EV) are considered potential therapeutic tools. Although CRC is a genetically heterogeneous disease, significance intra-tumor heterogeneity EV uptake cells not yet known. By using mouse and patient-derived organoids, currently available best model capturing cellular heterogeneity, we found that Apc mutation induced expression...
Extracellular vesicles (EV) are released by virtually all cells and they transport biologically important molecules from the release site to target cells. Colorectal cancer (CRC) is a leading cause of cancer-related death cases, thus, it represents major health issue. Although EV cargo may reflect molecular composition releasing EVs hold great promise for tumor diagnostics, impact intratumoral heterogeneity on intensity still largely unknown. By using CRC patient-derived organoids that...
Colorectal cancer (CRC) has a high incidence and is one of the leading causes cancer-related death. The accumulation cancer-associated fibroblasts (CAF) induces an aggressive, stem-like phenotype in tumor cells, it indicates poor prognosis. However, cellular heterogeneity among CAFs targeting both stromal CRC cells are not yet well resolved. Here, we identified CD142high with higher stimulating effect on cell proliferation via secreting more hepatocyte growth factor (HGF) compared to...
The ATP-Binding Cassette (ABC)-transporter MultiDrug Resistance Protein 1 (MDR1) and Multidrug Related (MRP1) are expressed on the surface of enterocytes, which has led to belief that these high capacity transporters responsible for modulating chemosensitvity colorectal cancer. Several immunohistochemistry reverse transcription polymerase chain reaction (RT-PCR) studies have provided controversial results in regards expression levels two ABC-transporters Our study was designed determine yet...
Liver is the third most common organ for breast cancer (BC) metastasis. Two main histopathological growth patterns (HGP) exist in liver metastases (LM): desmoplastic and replacement. Although a reduced immunotherapy efficacy reported patients with LM, tumor-infiltrating lymphocytes (TIL) have not yet been investigated BCLM. Here, we evaluate distribution of HGP TIL BCLM, their association clinicopathological variables survival. We collect samples from surgically resected BCLM (n = 133...
Phosphatase and tensin homolog deleted in chromosome 10 (PTEN) loss of function is frequently detected advanced colorectal cancer. Its detection thought to have prognostic significance it being considered predict responsiveness anti-EGFR therapy. Unfortunately, while immunohistochemical assessment PTEN expression widespread, lacks standardization the results are hardly comparable across available publications.Retrospectively collected, formalin-fixed paraffin-embedded tumor tissue samples...
Abstract Background: Liver metastases (LM) are ultimately present in ~50% of all patients with metastatic breast cancer (BC). Metastatic seeding to the liver relies on interaction between cells and host microenvironment, resulting into two main histopathological growth patterns (HGPs): replacement HGP (metastasis mimics architecture exploits vasculature) desmoplastic (presence a fibrotic rim separating hepatocytes from tumor cells). While prognostic value these HGPs is established for...
Besides clinicopathological parameters, molecular markers can be very important, and further characterize colorectal carcinomas into chromosomally unstable, microsatellite instable "CqG-island methylator phenotype" groups.To study the frequency of instability using immunohistochemical evaluation MLH1, MSH2, MSH6 PMS2 proteins in carcinoma.122 as well 69 paired liver metastases were evaluated. Additionally, prognostic predictive potential mismatch repair status was tested.Microsatellite...
Absztrakt Az autoimmun pancreatitis (AIP) egy ritka és pathomechanismusát tekintve ismeretlen betegség, amely az IgG4-asszociált betegségek családjába tartozik. Jól kezelhető steroiddal, bár a relapsus aránya 20–30%-ot is elérheti. Komoly differenciáldiagnosztikai problémát jelenthet azonban elkülönítése jóval gyakoribb pancreasdaganattól. esetek mintegy 20%-ában csak műtét utáni szövettani vizsgálat során derül ki AIP. A számtalan vizsgálóeljárás mindegyike hozzátesz valamit definitív...
The research group takes samples for molecular genetical examinations from tumors removed during operations within ischemic time interval. Samples are stored in liquid nitrogen. Clinical data of these patients recorded an informatics system developed by the group. Patients followed out-patient clinic set up this purpose not financed National Health Insurance Fund. Tissue and follow used to cooperate with laboratories.
A mesenterialis cysták ritka hasüregi elváltozások, amelyek jellegtelen panaszokat okozhatnak, és sokszor még a modern preoperatív képalkotó eljárások segítségével sem diagnosztizálhatóak egyértelműen. definitív ellátás sebészi reszekció, amely nyílt, laparoszkópos akár retroperitoneoszkópos technikával is elvégezhető. Műtéti indikáció áll fenn igazolt cysta esetén, ha okoz, növekszik, malignitás gyanúja merül fel. Sürgős beavatkozásra vérzés, volvulus, torquatio vagy bélelzáródás miatt...
Background: The ABC-transporters MDR1 and MRP1 are expressed in the normal mucosa of gastrointestinal tract. These proteins extruding various drugs from cells therefore can alter sensitivity colorectal cancer (CRC) – a phenomenon extensively studied previously by immune-histochemistry real-time PCR techniques. However, posttranslational modifications influence functional activity (MDR Activity Factor: MAF) MDR proteins, so determination bring new insights into understanding connection...
Introduction: About 0.2–1% of gastrointestinal tumours are stromal (GIST), which occur most frequently in the stomach, sometimes small intestine but they can develop rarely other parts tract as well. Mutation KIT gene has a prominent role its development, gain-of-function mutations commonly exons 11, 9 and 13. Not only surgical interventions, molecular biological methods have been available treatment recently.