A5064601650- Corneal Surgery and Treatments
- Retinal Diseases and Treatments
- Corneal surgery and disorders
- Intraocular Surgery and Lenses
- Ocular Surface and Contact Lens
- Glaucoma and retinal disorders
- Retinal Imaging and Analysis
- Retinal and Macular Surgery
- Retinal and Optic Conditions
- Neurogenesis and neuroplasticity mechanisms
- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Neuroscience and Neuropharmacology Research
- Connexins and lens biology
- Ophthalmology and Visual Impairment Studies
- Ocular Infections and Treatments
- Ocular Oncology and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Anesthesia and Neurotoxicity Research
- Tissue Engineering and Regenerative Medicine
- Ocular Disorders and Treatments
- Angiogenesis and VEGF in Cancer
- Ocular Diseases and Behçet’s Syndrome
- Mesenchymal stem cell research
- Glioma Diagnosis and Treatment
Oslo University Hospital
2016-2025
University of Oslo
2016-2025
Queen's University Belfast
2023
657 Oslo
2023
Karolinska Institutet
2005-2008
University of Southern California
2003
Neurological Surgery
2003
The complex immunological mechanisms of vaccines bring about an inevitable risk immune-mediated adverse reactions.Of special interest in this time epidemic is the safety COVID-19 and, particular, emerging evidence that ChAdOx1 nCoV-19 adenoviral vector vaccine from AstraZeneca can cause vaccine-induced immune thrombotic thrombocytopenia (VITT).We present case a patient who developed acute paracentral scotoma after having received vaccine.A 27-year-old female was referred for ophthalmology...
The human albumin variant E505Q/T527M/K573P improves pharmacokinetics of protein-based drugs and facilitates transport across mucosal epithelia.
It was long held as an axiom that new neurons are not produced in the adult human brain. More recent studies have identified multipotent cells whose progeny express glial or neuronal markers. This discovery may lead to therapeutic strategies for CNS disorders, either by stimulating neurogenesis vivo transplanting progenitor (MPCs) been propagated and differentiated vitro. The clinical application of such approaches will be limited ability these develop into functional neurons. To facilitate...
Abstract Multiple sclerosis is an inflammatory disease of the central nervous system characterized by inflammation, demyelination, axonal degeneration and accumulation neurological disability. Previously, we demonstrated that stem cells constitute a possible endogenous source for remyelination. We now addressed question whether neurogenesis can occur in neuroinflammatory lesions. that, experimental autoimmune encephalomyelitis, induced rats...
Abstract Corneal tissue regeneration is of crucial importance for maintaining normal vision. We aimed to isolate and cultivate human corneal stroma-derived mesenchymal stem-like cells (CSMSCs) from the central part cadaver corneas study their phenotype, multipotency, role in immunity wound healing. The isolated grew as monolayers vitro , expressed mesenchymal- stemness-related surface markers (CD73, CD90, CD105, CD140b) were negative hematopoietic determined by flow cytometry. CSMSCs able...
Editor, Since the end of 2019, an outbreak a novel coronavirus (SARS-CoV-2) originating in Chinese city Wuhan has rapidly spread across world. The disease is designated COVID-19, abbreviation for 2019 (World Health Organization 2020a, 2020b). While most patients experience mild sickness or are asymptomatic, COVID-19 can lead to severe respiratory illness and even death (Wu & McGoogan 2020). In February 2020, epidemic reached Norway; ground zero was Department Ophthalmology at Oslo University...
Purpose: Explore in vivo whether there is direct communication between the cerebrospinal fluid (CSF) and extravascular compartment of human visual pathway structures. Methods: A prospective observational study included 10 subjects who underwent intrathecal gadolinium–enhanced magnetic resonance imaging (MRI) for suspected CSF circulation disorder, but with a negative result no known ophthalmic diseases. After precontrast T1-weighted MRI, 0.5 mL gadobutrol (Gadovist, 1.0 mmol/mL) was injected...
Purpose: To investigate the mechanism by which resveratrol acts upon retinal pigment epithelial (RPE) cells and to characterize its effect autophagy, survival, inflammation, with consequent implications treatment for age-related macular degeneration (AMD).Cultured ARPE-19 were exposed 10 50 μM resveratrol. Cell survival/death was determined annexin-FITC/propidium iodide using flow cytometry, while autophagy studied detecting autophagic vacuoles formation (acridine orange transmission...
Injury of the cornea is a complex biological process. Regeneration corneal stroma can be facilitated by presence mesenchymal stromal cells (MSCs) and application tissue equivalents. A new tissue-engineering strategy for regeneration presented using cellularized 3D bioprinted hydrogel constructs implanted into organ cultured porcine corneas femtosecond laser-assisted intrastromal keratoplasty. The ex vivo cultured, MSC-loaded structures remain intact, support cell survival, contain de novo...
A simple, reproducible, animal-material free method for cultivating and characterizing cornea limbal epithelial stem cells (LESCs) on human lens capsule (LC) was developed future clinical transplantation. The tissue explants (2×2×0.25 mm) were harvested from 77 cadavers expanded ex vivo either cell culture plates or LC in medium containing serum as the only growth supplement. Cell outgrowth at edge of observed within 24 hours cultivation achieved viable (>97% viability measured by MTT assay...
Regeneration of cells in the central nervous system is a process that might be affected during neurological disease and trauma. Because nitric oxide (NO) its derivatives are powerful mediators inflammatory cascade, we have investigated effects pathophysiological concentrations NO on neurogenesis, gliogenesis, expression proneural genes primary adult neural stem cell cultures. After exposure to NO, neurogenesis was downregulated, this corresponded decreased gene neurogenin-2 beta-III-tubulin....
OBJECTIVE To directly compare stem cells from the normal adult human brain (adult neural [AHNSC]), Grade II astrocytomas (AC II), and glioblastoma multiforme (GBM), with respect to proliferative tumor-forming capacity differentiation potential. METHODS Cells were isolated tissue obtained during epilepsy surgery (AHNSCs) or tumor (glioma [GSC]). They cultured investigated in vitro after transplantation immunodeficient mice. RESULTS Under identical experimental conditions, following found: 1)...
Purpose: DNA damage has been described in the human cataractous lens epithelium, and oxidative stress generated by UV radiation endogenous metabolic processes suggested to play a significant role pathogenesis of cataract. In this study, aim was explore quality relative quantity epithelium cataract patients vivo after incubation cell culture system. Methods: Capsulotomy specimens were analysed, before 1 week ex cultivation, using comet assay measure strand breaks, oxidized purine pyrimidine...
Intravitreal injections of antibody-based biologics targeting vascular endothelial growth factor (VEGF) are highly effective and have markedly decreased the risk visual impairment associated with prevalent retinal diseases, such as neovascular age-related macular degeneration diabetes oedema. The diseases chronic in their nature, most patients need long-term therapy to suppress disease activity. We previously reported a compounding method for repackaging storage aflibercept (Eylea), commonly...
To investigate the migration, proliferation, and differentiation of stem cells neural progenitor (NPCs) from adult human brain after transplantation into rodent brains.Adult NPCs were obtained temporal lobe specimens removed because medical intractable epilepsy. The transplanted posterior periventricular region above hippocampus in brains either healthy rats (control) or with selective injury hippocampal CA1 (global ischemia).In control animals, grafted mainly distributed site toward midline...
OBJECTIVE: It was long held as an axiom that new neurons are not produced in the adult human brain. More recent studies, however, have identified multipotent cells whose progeny express glial or neuronal markers. This discovery may lead to therapeutic strategies against central nervous system disorders by transplanting stem been propagated vitro. Still, it is known whether from brain retain potential mature into integrate and communicate a network. METHODS: We cultured ventricular wall of...
Purpose: Presently, our clinic is the only centre in Scandinavia that offers patients with corneal surface pathology including limbal stem cell deficiency (LSCD) transplantation of ex vivo expanded epithelial cells (LECs). We here present clinical data first nine LSCD who were transplanted autologous LECs medium completely free any animal-derived products and non-human/recombinant growth factors (including Cholera Toxin), human serum as supplement. Methods: conducted a noncomparative...