A5006605910- Immune Cell Function and Interaction
- Advanced Fluorescence Microscopy Techniques
- T-cell and B-cell Immunology
- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Ion channel regulation and function
- Adipokines, Inflammation, and Metabolic Diseases
- Genomics and Chromatin Dynamics
- Drug Transport and Resistance Mechanisms
- DNA Repair Mechanisms
- Nanoparticle-Based Drug Delivery
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Photosynthetic Processes and Mechanisms
- Analytical Chemistry and Sensors
- Monoclonal and Polyclonal Antibodies Research
- Spectroscopy and Chemometric Analyses
- Chemokine receptors and signaling
- Neuroscience and Neuropharmacology Research
- Water Quality Monitoring and Analysis
- Cytokine Signaling Pathways and Interactions
- Toxin Mechanisms and Immunotoxins
- Venomous Animal Envenomation and Studies
- Landslides and related hazards
University of Debrecen
2009-2024
Eötvös Loránd University
2021
Research Network (United States)
2021
Hungarian Research Network
2021
Hungarian Academy of Sciences
2007-2009
Activation of poly(ADP-ribose) polymerase-1 (PARP1) has been shown to mediate cell death induced by genotoxic stimuli. The role glycohydrolase (PARG), the enzyme responsible for polymer degradation, largely unexplored in regulation death. Using lentiviral gene silencing we generated A549 lung adenocarcinoma lines with stably suppressed PARG and PARP1 expression (shPARG shPARP1 lines, respectively) determined parameters apoptotic necrotic following hydrogen peroxide exposure. shPARG cells...
With the evolving technology in CMOS integration, new classes of 2D-imaging detectors have recently become available. In particular, single photon avalanche diode (SPAD) arrays allow detection photons at high acquisition rates (\geq 100 kfps), which is about two orders magnitude higher than with currently available cameras. Here we demonstrate use a SPAD array for imaging fluorescence correlation spectroscopy (imFCS), tool to create 2D maps dynamics fluorescent molecules inside living cells....
ABCG2 is an exporter-type ABC protein that can expel numerous chemically unrelated xeno- and endobiotics from cells. When expressed in tumor cells or stem cells, confers multidrug resistance, contributing to the failure of chemotherapy. Molecular details orchestrating substrate translocation ATP hydrolysis remain elusive. Here, we present methods concomitantly investigate nucleotide binding by Using conformation-sensitive antibody 5D3, show switch inward-facing (IF) outward-facing (OF)...
Significance Interleukin-2 (IL-2), a cytokine produced by activated T cells, acting in paracrine/autocrine fashion, is essential for cell proliferation. In adult leukemia (ATL) and autoimmune/graft-vs.-host diseases, anti–IL-2Rα antibody therapies were established to inhibit proliferation, with only limited success. We demonstrated that IL-2/15 receptors start assemble the endoplasmic reticulum/Golgi signaling Jak1/Jak3 Golgi. Thus, cells also producing IL-2 (e.g., ATL cells), can already...
Thermogenic brown and beige adipocytes oxidize metabolic substrates producing heat, mainly by the mitochondrial uncoupling protein UCP1, can thus counteract obesity. Masked possess white adipocyte-like morphology, but be made thermogenic adrenergic stimuli. We investigated regulation of mitophagy upon activation human masked mature adipocytes. Human primary abdominal subcutaneous adipose-derived stromal cells (hASCs) Simpson–Golabi–Behmel syndrome (SGBS) preadipocytes were differentiated to...
We observed prominent effects of doxorubicin (Dox), an anthracycline widely used in anti-cancer therapy, on the aggregation and intracellular distribution both partners H2A-H2B dimer, with marked differences between two histones. Histone aggregation, assessed by Laser Scanning Cytometry via retention aggregates isolated nuclei, was case H2A. The dominant effect H2B its massive accumulation cytoplasm Jurkat leukemia cells concomitant disappearance from detected confocal microscopy mass...
Abstract Doxorubicin (Dox), a widely used anticancer DNA-binding drug, affects chromatin in multiple ways, and these effects contribute to both its efficacy dose-limiting side effects, especially cardiotoxicity. Here, we studied the of Dox on binding architectural proteins high mobility group B1 (HMGB1) linker histone H1, transcription factor retinoic acid receptor (RARα) by fluorescence recovery after photobleaching (FRAP) correlation spectroscopy (FCS) live cells. At lower doses, increased...
Introduction: White adipocytes store lipids, have a large lipid droplet and few mitochondria. Brown beige adipocytes, which produce heat, are characterized by high expression of uncoupling protein (UCP) 1, multilocular droplets, amounts The rs1421085 T-to-C single-nucleotide polymorphism (SNP) the human FTO gene interrupts conserved motif for ARID5B repressor, resulting in adipocyte type shift from to white. Methods: We obtained abdominal subcutaneous adipose tissue donors carrying TT...
Abstract The voltage-gated proton channel Hv1 is widely expressed, among others, in immune and cancer cells, it provides an efficient cytosolic H + extrusion mechanism regulates vital functions such as oxidative burst, migration proliferation. Here we demonstrate the presence of human (hHv1) placenta/chorion-derived mesenchymal stem cells (cMSCs) using RT-PCR. voltage- pH-dependent gating current similar to that hHv1 expressed cell lines blocked by 5-chloro-2-guanidinobenzimidazole (ClGBI)...
The importance of membrane rafts in HIV-1 infection is still the focus interest. Here, we report that new monoclonal anticholesterol IgG antibodies (ACHAs), recognizing clustered cholesterol (e.g., lipid rafts), rearrange lateral molecular organization receptors and coreceptors plasma permissive human T-cells macrophages. This remodeling accompanied with a substantial inhibition their production vitro. ACHAs promote association CXCR4 both CD4 rafts, consistent decreased mobility CXCR4, while...
We introduce a new multiple-tau hardware correlator design for computing fluorescence correlation functions (CFs) in real time. Use of resources is minimized by scheduling the computation different segments CFs on single block. Simultaneous calculation two autocorrelation (ACFs) and cross-correlation (CCFs) implemented LabVIEW National Instruments field programmable gate array (FPGA) card with minimal sampling time 400 ns. Raw data are stored resolution 50 The can be easily adapted to other...
Single Plane Illumination Microscopy (SPIM) revolutionized time lapse imaging of live cells and organisms due to its high speed reduced photodamage. Quantitative mapping molecular (co)mobility by fluorescence (cross-)correlation spectroscopy (F(C)CS) in a SPIM has been introduced reveal diffusion binding. A complementary aspect interactions is proximity, which can be studied Förster resonance energy transfer (FRET). Here, we extend SPIM-FCCS alternating laser excitation, reduces false...
H2A.Z-nucleosomes are present in both euchromatin and heterochromatin it has proven difficult to interpret their disparate roles the context of stability features. Using an situ assay nucleosome DT40 cells expressing engineered forms histone variant we show that native H2A.Z, but not C-terminally truncated H2A.Z (H2A.Z∆C), is released from nucleosomes peripheral at unusually high salt concentrations. H3K9me3 landscapes reorganized H2A.Z∆C-nuclei overall sensitivity chromatin nucleases...
Brown and beige adipocytes have multilocular lipid droplets, express uncoupling protein (UCP) 1, promote energy expenditure. In rodents, when the stimulus of browning subsides, parkin-dependent mitophagy is activated dormant persist. humans, however, molecular events during to white transition not been studied in detail. this study, human primary subcutaneous abdominal preadipocytes were differentiated for 14 days, then either culture conditions applied an additional days or it was replaced...
Every day, billions of our cells die and get cleared without inducing inflammation. When, clearance is improper, uncleared undergo secondary necrosis trigger In addition, proper efferocytosis would be required for resolution inflammation, thus deficiencies in the long term lead to development various chronic inflammatory diseases. Increasing evidence indicates that obesity, itself being a low-grade disease, predisposes variety other Previous studies indicated this later might partially...
Abstract IL-15 plays a pivotal role in the long-term survival of T cells and immunological memory. Its receptor consists three subunits (IL-15Rα, IL-2/15Rβ, γc). functions mainly via trans-presentation (TP), during which an APC expressing bound to IL-15Rα presents ligand βγc receptor-heterodimer on neighboring T/NK cell. To date, no direct biophysical evidence for intercellular assembly IL-15R heterotrimer exists. Ag presentation (AP), initial step cell activation, is also based APC–T...
Upregulation of the voltage-gated potassium channel KV1.3 is implicated in a range autoimmune and neuroinflammatory diseases, including rheumatoid arthritis, psoriasis, multiple sclerosis, type I diabetes. Understanding expression, localization, trafficking normal disease states key to developing targeted immunomodulatory therapies. HsTX1[R14A], an analogue 34-residue peptide toxin from scorpion Heterometrus spinifer, binds with high affinity (IC50 45 pM) selectivity (2000-fold for over...
Voltage-gated Kv1.3 potassium channels are essential for maintaining negative membrane potential during T-cell activation. They interact with membrane-associated guanylate kinases (MAGUK-s) via their C-terminus and TCR/CD3, leading to enrichment at the immunological synapse (IS). Molecular interactions mobility may impact each other function of these proteins. We aimed identify molecular determinants mobility, applying fluorescence correlation spectroscopy on human Jurkat T-cells expressing...
The heterodimeric receptor complex of IL-9 consists the cytokine-specific α-subunit and common γc -chain shared with other cytokines, including IL-2, a central regulator T cell function. We have shown previously bipartite spatial relationship IL-2 receptors at surface human lymphoma cells: in addition to clusters, expression two kinds could also be observed segregated membrane areas. Here we analyzed further mutual organization receptors. Complementing Pearson correlation data co-occurrence...
Abstract Earlier we found that interleukin-2 and -15 receptors (IL-2R, IL-15R) MHC I II, all proteins essential in T cell activation, form co-clusters at different hierarchic levels lipid rafts of cells. To investigate whether the membrane-associated actin cytoskeleton has a role organizing these clusters, used membrane blebs formed spontaneously on starved human lymphoma cells for comparison, as they have smooth surface without cytoskeletal connections. STED microscopy showed patchy...