A5000414691- Drug Transport and Resistance Mechanisms
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Trace Elements in Health
- HIV/AIDS drug development and treatment
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- Tryptophan and brain disorders
- Glycosylation and Glycoproteins Research
- Pharmacological Effects and Toxicity Studies
- Protein Interaction Studies and Fluorescence Analysis
- Reproductive System and Pregnancy
- Genomics and Chromatin Dynamics
- Caveolin-1 and cellular processes
- Fish Ecology and Management Studies
- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Reproductive Biology and Fertility
- Antibiotics Pharmacokinetics and Efficacy
- Amino Acid Enzymes and Metabolism
- Retinoids in leukemia and cellular processes
- Stress Responses and Cortisol
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Cell Adhesion Molecules Research
University of Debrecen
2011-2023
Olympus (Japan)
2009
GeneCare Research Institute (Japan)
2009
Osaka University
1993-2003
Kobe Gakuin University
1987-1999
Abstract Background A novel flow cytometric assay has been described in an accompanying report (Gombos et al., Methods The kinetics of the decrease immunofluorescence intensity was analyzed after addition raft‐preserving Triton X‐100 or Nonidet P‐40, both which disrupt entire membrane. Mild treatments by detergents leave cells attached to only those proteins that are anchored cytoskeleton rafts independent rafts. Agents affect microfilaments and modulate membrane levels cholesterol...
Abstract P-glycoprotein (Pgp) is an ABC transporter responsible for the ATP-dependent efflux of chemotherapeutic compounds from multidrug resistant cancer cells. Better understanding molecular mechanism Pgp-mediated transport could promote rational drug design to circumvent resistance. By measuring binding affinity and reactivity a conformation-sensitive antibody we show here that nucleotide drives Pgp high low substrate-affinity state this switch coincides with flip inward- outward-facing...
Two species from the families Acipenseridae and Polyodontidae, Russian sturgeon (Acipenser gueldenstaedtii, Brandt Ratzeberg, 1833; functional tetraploid) American paddlefish (Polyodon spathula, Walbaum 1792, diploid) were hybridized. The hybridization was repeated using eggs three sperm four individuals. Survival in all hybrid family groups ranged 62% to 74% 30 days after hatching. This first successful between these two members of Polyodontidae. Flow cytometry chromosome analysis revealed...
ABCG2 is an exporter-type ABC protein that can expel numerous chemically unrelated xeno- and endobiotics from cells. When expressed in tumor cells or stem cells, confers multidrug resistance, contributing to the failure of chemotherapy. Molecular details orchestrating substrate translocation ATP hydrolysis remain elusive. Here, we present methods concomitantly investigate nucleotide binding by Using conformation-sensitive antibody 5D3, show switch inward-facing (IF) outward-facing (OF)...
We reported earlier that neonatal monocyte-derived macrophages (MDM) could not be fully activated with IFN-gamma, a finding attributed to lower expression of IFN-gamma receptors on the cells. In this study we explored elements R-mediated signalling in cord monocytes and MDM. Intracellular STAT-1 was analysed by flow cytometry. have assessed phosphorylation using MoAbs distinguish native phosphorylated forms discrete cell basis. Using against form revealed comparable protein adult cells (both...
Abstract A number of cell functions, such as flagellar beating, swimming velocity, acrosome reaction, etc., are triggered by a Ca 2+ influx across the membrane. For appropriate physiological motile human sperm maintains intracellular free calcium concentration ([Ca ] i ) at submicromolar level. The objective this study was to determine role Na + /Ca exchanger (NCX) in maintenance [Ca spermatozoa. Spermatozoa maintained extracellular medium containing ≥1 μM exhibited motility similar that...
Human ABCG2 is a plasma membrane glycoprotein working as homodimer or homo-oligomer. The protein plays an important role in the protection/detoxification of various tissues and may also be responsible for multidrug-resistant phenotype cancer cells. In our previous study we found that 5D3 monoclonal antibody shows function-dependent reactivity to extracellular epitope transporter. current experiments have further characterized 5D3-ABCG2 interaction. effect chemical cross-linking modulation...
P-glycoprotein (Pgp) is one of the active efflux pumps that are able to extrude a large variety chemotherapeutic drugs from cells, causing multidrug resistance. The conformation-sensitive UIC2 monoclonal antibody potentially inhibits Pgp-mediated substrate transport. However, this inhibition usually partial, and its extent variable because binds only 10 40% Pgp present in cell membrane. rest molecules become recognized by presence certain substrates or modulators, including vinblastine,...
There is evidence that reperfusion injury of cardiac tissue may be caused by the generation oxygen-derived free radicals and oxidants induction intracellular calcium overload, although relation between these two mechanisms uncertain. In addition, types cellular specific active species unclear. an attempt to resolve problems, we investigated effects oxygen oxidants, which are purportedly generated during after prolonged ischemia, various antioxidants on contractility morphology cultured fetal...
Retinoids - derivatives of vitamin A are important cell permeant signaling molecules that regulate gene expression through activation nuclear receptors. P-glycoprotein (Pgp) and ABCG2 plasma membrane efflux transporters affecting the tissue distribution numerous structurally unrelated lipophilic compounds. In present work we aimed to study interaction above ABC with retinoid derivatives. We have found 13-cis-retinoic acid, retinol retinyl-acetate inhibited Pgp mediated substrate transport as...
Conformational changes accompanying P‐glycoprotein (Pgp) mediated drug transport are reflected by in the avidity of certain monoclonal antibodies (mAbs). More UIC2 mAb binds to Pgp‐expressing cells presence substrates or modulators [Mechetner, E.B., Schott, B., Morse, S.B., Stein, W., Druley, T., Dvis, K.A., Tsuruo, T. & Roninson, I.B. (1997) Proc. Natl Acad. Sci. USA 94 , 12908–12913], while binding other mAbs (e.g. MM12.10, MRK16, 4E3) is not conformation sensitive. Pre‐staining Pgp +...
Abstract Background Lipid rafts are cholesterol‐ and glycosphingolipid‐rich microdomains in the cellular plasma membranes that play critical roles compartmentalization (concentration, coupling, isolation) of receptors signal molecules. Therefore, detecting constitutive or induced raft associations such proteins is central interest cell biology. This has mostly been done with time‐ cell‐consuming immunobiochemical techniques affected by several sources artifacts. A flow cytometric analysis...
Several ABC exporters carry a degenerate nucleotide binding site (NBS) that is unable to hydrolyze ATP at rate sufficient for sustaining transport activity. A hallmark of NBS the lack catalytic glutamate in Walker B motif domain (NBD). The multidrug resistance transporter ABCB1 (P-glycoprotein) has two canonical NBSs, and mutation E556 NBS1 renders transport-incompetent. In contrast, closely related bile salt export pump ABCB11 (BSEP), which shares 49% sequence identity with ABCB1, naturally...
By using a microscopic approach, field inversion single-cell gel electrophoresis, we show that preformed single-strand discontinuities are present in the chromatin of resting and proliferating mammalian yeast cells. These breaks primarily nicks positioned at approximately 50-kbp intervals throughout entire genome could be efficiently labeled situ by DNA polymerase I holoenzyme but not Klenow fragment terminal transferase unless after ribonucleolytic treatments. The RNA molecules involved...