A5066034218- Chronic Lymphocytic Leukemia Research
- Drug Transport and Resistance Mechanisms
- Lymphoma Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- HIV/AIDS drug development and treatment
- Amino Acid Enzymes and Metabolism
- Trace Elements in Health
- Nanoparticle-Based Drug Delivery
- Retinoids in leukemia and cellular processes
- Multiple Myeloma Research and Treatments
- Acute Myeloid Leukemia Research
- Peroxisome Proliferator-Activated Receptors
- Cancer, Lipids, and Metabolism
- Single-cell and spatial transcriptomics
- Biosensors and Analytical Detection
- Cholesterol and Lipid Metabolism
- ATP Synthase and ATPases Research
- Cystic Fibrosis Research Advances
- Pluripotent Stem Cells Research
- Clinical Laboratory Practices and Quality Control
- RNA Interference and Gene Delivery
- Mine drainage and remediation techniques
- Calcium signaling and nucleotide metabolism
- Water Quality and Pollution Assessment
- Nuclear Receptors and Signaling
Semmelweis University
2018-2025
HUN-REN Institute of Experimental Medicine
2022
University of Szeged
2021
National Institute of Oncology
2021
University of Debrecen
2013-2020
Abstract P-glycoprotein (Pgp) is an ABC transporter responsible for the ATP-dependent efflux of chemotherapeutic compounds from multidrug resistant cancer cells. Better understanding molecular mechanism Pgp-mediated transport could promote rational drug design to circumvent resistance. By measuring binding affinity and reactivity a conformation-sensitive antibody we show here that nucleotide drives Pgp high low substrate-affinity state this switch coincides with flip inward- outward-facing...
Retinoids - derivatives of vitamin A are important cell permeant signaling molecules that regulate gene expression through activation nuclear receptors. P-glycoprotein (Pgp) and ABCG2 plasma membrane efflux transporters affecting the tissue distribution numerous structurally unrelated lipophilic compounds. In present work we aimed to study interaction above ABC with retinoid derivatives. We have found 13-cis-retinoic acid, retinol retinyl-acetate inhibited Pgp mediated substrate transport as...
Several ABC exporters carry a degenerate nucleotide binding site (NBS) that is unable to hydrolyze ATP at rate sufficient for sustaining transport activity. A hallmark of NBS the lack catalytic glutamate in Walker B motif domain (NBD). The multidrug resistance transporter ABCB1 (P-glycoprotein) has two canonical NBSs, and mutation E556 NBS1 renders transport-incompetent. In contrast, closely related bile salt export pump ABCB11 (BSEP), which shares 49% sequence identity with ABCB1, naturally...
Diabetes mellitus-related morbidity and mortality is a rapidly growing healthcare problem, globally. Several nutraceuticals exhibit potency to target the pathogenesis of diabetes mellitus. The antidiabetic effects compounds garlic have been extensively studied, however, limited data are available on biological certain component, allithiamine. In this study, allithiamine was tested using human umbilical cord vein endothelial cells (HUVECs) as hyperglycaemic model. HUVECs were isolated by...
Abstract Objectives CD49f is an adhesion molecule present on malignant lymphoblasts in B-cell acute lymphoblastic leukemia; it associated with a poor prognosis. expression has been proposed as marker for measurable residual disease (MRD) marker, but this yet to be implemented clinical practice. Methods In study, we used flow cytometry detect by leukemic blasts paired bone marrow and cerebrospinal fluid samples at diagnosis day 15 of treatment. Results At diagnosis, 93% 100% expressed CD49f....
Expression of multidrug pumps including P-glycoprotein (MDR1, ABCB1) in the plasma membrane tumor cells often results decreased intracellular accumulation anticancer drugs causing serious impediment to successful chemotherapy. It has been shown earlier that combined treatment with UIC2 anti-Pgp monoclonal antibody (mAb) and cyclosporine A (CSA) is an effective way blocking Pgp function. In present work we investigated suitability four PET diagnostic radiotracers 2-[ 18...
P-glycoprotein (Pgp) extrudes a large variety of chemotherapeutic drugs from the cells, causing multidrug resistance (MDR). The UIC2 monoclonal antibody recognizes human Pgp and inhibits its drug transport activity. However, this inhibition is partial, since binds only to 10–40% cell surface Pgps, while rest becomes accessible in presence certain substrates or modulators (e.g. cyclosporine A (CsA)). combined addition 10 times lower concentrations CsA than what necessary for when modulator...
Abstract Context DNA demethylation and inhibitory effects of aspirin on pituitary cell proliferation have been demonstrated. Objective Our aim was to clarify the molecular mechanisms behind aspirin-related in cells. Methods methylome whole transcriptome profile were investigated RC-4B/C GH3 lines upon treatment. Effects a agent, decitabine, further tested vitro. PTTG1 expression 41 human PitNET samples genome gene protein data 76 34 control (available Gene Expression Omnibus) evaluated....
Background: Ibrutinib is widely known as an effective and well-tolerated therapeutical choice of the chronic lymphocytic leukaemia (CLL). However, acquired resistance may occur during treatment, causing relapse. Early detection ibrutinib important issue, therefore we aimed to find phenotypic markers on CLL cells expression which correlate with appearance resistance. Methods: We examined 28 patients' peripheral blood (PB) samples (treatment naïve, sensitive, clinically resistant). The surface...
MiR-206 is a remarkable miRNA because it functions as suppressor in rhabdomyosarcoma while at the same time, previously showed, can act an oncomiRNA SMARCB1 immunonegative soft tissue sarcomas. The aim of this study was to investigate effect miR-206 on its several target genes various human tumorous and normal cell lines. In current work, we created miR-206-overexpressing lines (HT-1080, Caco2, iASC, SS-iASC) using permanent transfection. mRNA expression (SMARCB1, ACTL6A, CCND1, POLA1,...
The Sleeping Beauty transposon system was established as a robust and efficient method for germline transgenesis in different mammalian species. generation of transgenic mice, rats, rabbits swine carrying an identical Venus reporter construct delivered by transposon-mediated gene transfer enables comparative studies expression these lines models. Whereas comparable patterns the were found somatic tissues, preliminary suggested that striking difference may exist mature spermatozoa Here we...
The Bcl-2 inhibitor venetoclax has been recently introduced into the treatment of chronic lymphocytic leukemia. Venetoclax is a highly effective drug, however acquired resistance may make long-term challenging. In our study, we present potential novel mechanisms and prognostic markers that are potentially able to predict early appearance resistance.Repeated complete blood counts, flow cytometric measurements, physical examinations were performed during patient follow-up. Clinical laboratory...
A nagy granuláris lymphocyta (LGL) leukémia leggyakrabban normál LGL-sejtek expanzióját kiváltó krónikus antigén stimulációt okozó állapotokkal, mint például vírus infekcióval, autoimmun betegségekkel és elsősorban hematológiai malignitásokkal társul. bemutatott esetben az LGL-leukémia egy ritka okát, tünetét diagnosztikai lehetőségeit tárgyaljuk. várandós nőbetegnél rutin vérvizsgálat során észlelt thrombocytosis splenomegália hátterében chronikus myeloid igazolódott. Az interferon...
Plasma cell enrichment plays a pivotal role in the accurate prognosis and molecular characterization of multiple myeloma. The separation is commonly carried out by positive selection using CD138 monoclonal antibody conjugated to magnetic beads. Optimally, during procedure, cells should neither be damaged, nor their phenotype significantly altered, as these changes would falsify results if isolated were subsequently used. For this reason, we investigated expression patterns different surface...
Abstract In the pathogenesis of chronic lymphocytic leukemia (CLL) microenvironment plays an important role, as it produces survival signals and mediates drug resistance. Lenalidomide, which has immunomodulatory effect, can enhance activation T‐, NK‐cells endothelial cells, however there are no data available whether modulate bone marrow stromal cells (BMSCs). our study, we investigated effects lenalidomide on BMSCs CLL cells. were cultured alone or with treated lenalidomide. Apoptosis,...
Plasma cell myeloma (multiple [MM]) is a malignant neoplasm originating from the plasma cells. Besides other methods, flow cytometric analysis of patient's bone marrow aspirate has an important role in diagnosis and also response assessment. Since surface markers, used for identifying abnormal cells, are expressed diversely treatment can alter phenotype there increasing demand new markers. VS38c monoclonal antibody that recognizes CLIMP-63 protein membrane endoplasmic reticulum. known to be...
Absztrakt: A krónikus limfoid leukémia (CLL) a leggyakoribb felnőttkori fejlett országokban. CLL egy indolens lefolyású, gyógyíthatatlan betegség, mely kezelést követő relapszusokkal járhat, emiatt betegek folyamatos követése szükséges. Az elmúlt évek bővülő terápiás lehetőségei (pl.: alemtuzumab, venetoclax) lehetővé tették daganatos sejtek mennyiségének tartós és drasztikus csökkenését, ezért szükségessé vált visszatérő betegség minél érzékenyebb detektálása. Erre nyújt lehetőséget...
Flow cytometry is a standard tool to detect protein expression, DNA content, and other cell physiological parameters at single level (1-3). Since flow cytometers are capable of processing suspensions, they excellent instruments study fluid tissue samples like blood or bone marrow aspirates. This makes very useful in hematology immunology. In human samples, the cells interest usually leukocytes, therefore largest portion formed elements, anucleated erythrocytes, platelets, undesired samples....