A5081690721- Radiopharmaceutical Chemistry and Applications
- Peptidase Inhibition and Analysis
- Medical Imaging Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Drug Transport and Resistance Mechanisms
- Lanthanide and Transition Metal Complexes
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Metabolism, Diabetes, and Cancer
- Muscle Physiology and Disorders
- Advanced MRI Techniques and Applications
- Lung Cancer Treatments and Mutations
- Inflammatory mediators and NSAID effects
- Neuropeptides and Animal Physiology
- Drug Solubulity and Delivery Systems
- Radiomics and Machine Learning in Medical Imaging
- MRI in cancer diagnosis
- Prostate Cancer Treatment and Research
- Crystallization and Solubility Studies
- Amino Acid Enzymes and Metabolism
- Photodynamic Therapy Research Studies
- Glioma Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
University of Debrecen
2015-2024
Active Medicine (United Kingdom)
2021
Faculty (United Kingdom)
2021
Smart/intelligent contrast agent candidates for MRI based on Mn(II) ion are rare, as it usually forms labile complexes with polyaminocarboxylate-type ligands. Here, we report the first example of a complex that can be activated by changing pH its local environment. The PC2A-EA ligand an ethylamine pendant arm was found to form thermodynamically stable (log KMnL = 19.01, pMn 9.27) and kinetically inert respect trans-chelation metal such Cu(II). [MnH(PCA2-EA)] displays relatively slow water...
Objective— Vascular calcification is associated with high risk of cardiovascular events and mortality. Osteochondrogenic differentiation vascular smooth muscle cells (VSMCs) the major cellular mechanism underlying calcification. Because tissue hypoxia a common denominator in calcification, we investigated whether per se triggers osteochondrogenic VSMCs. Approach Results— We studied human aorta VSMCs cultured under normoxic (21% O 2 ) hypoxic (5% conditions. Hypoxia increased protein...
Toxicity concerns related to Gd(III)-based magnetic resonance imaging (MRI) agents prompted an intensive research toward their replacement by complexes of essential metal ions, like Mn(II). Here, we report a macrocyclic chelate, [Mn(PC2A-BP)], which possesses high thermodynamic stability (log KMnL = 14.86 and pMn=8.35) kinetic inertness (t1/2pH=7.4 286.2 h) as well remarkable relaxivity (r1p 23.5 mM-1 s-1, 0.49 T, 37 °C) in the presence human serum albumin, allowing significant MRI signal...
Targeted alpha-particle therapy using radionuclides with alpha emission is a rapidly developing area in modern cancer treatment. To selectively deliver alpha-emitting isotopes to tumors, targeting vectors, including monoclonal antibodies, peptides, small molecule inhibitors, or other biomolecules, are attached them, which ensures specific binding tumor-related antigens and cell surface receptors. Although earlier studies have already demonstrated the anti-tumor potential of radium (Ra)...
Abstract Unprecedented fast and efficient complexation of Sc III was demonstrated with the chelating agent AAZTA (AAZTA=1,4‐bis(carboxymethyl)‐6‐[bis(carboxymethyl)]amino‐6‐methylperhydro‐1,4‐diazepine) under mild experimental conditions. The robustness 44 Sc(AAZTA) − chelate conjugated biomolecules thereof is further shown by in vivo PET imaging healthy tumor mice models. new results pave way towards development Sc‐based radiopharmaceuticals using chelator.
This study aimed to develop a novel radiotracer using trastuzumab and the long-lived [52Mn]Mn isotope for HER2-targeted therapy selection monitoring. A new Mn(II) chelator, BPPA, synthesized from rigid bispyclen platform possessing picolinate pendant arm, formed stable inert complex with favorable relaxation properties. BPPA was converted into bifunctional chelator (BFC), conjugated trastuzumab, labeled isotope. In comparison DOTA-GA-trastuzumab, BPPA-trastuzumab conjugate exhibits labeling...
Key points The in situ phenotypic switch of macrophages is delayed acute injury following irradiation. combination bone marrow transplantation and local muscle radiation protection allows for the identification a myeloid cell contribution to tissue repair. PET‐MRI monitoring invasion metabolism. Altered cellular composition prior sterile affects transition invading cells repair macrophages. There reciprocal intercellular communication between compartments, such as PAX7 positive cells,...
Electrospinning has recently been recognized as a potential method for use in biomedical applications such nanofiber-based drug delivery or tissue engineering scaffolds. The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) bone regeneration under vitro vivo conditions. mesh physicochemical properties included 147 ± 50 nm structure, aqueous media...
Until the recent years, substances containing radioactive
Seminal vesicle secretion 2 (SVS2) is a protein secreted by the mouse seminal vesicle. We previously demonstrated that SVS2 regulates fertilization in mice; attached to ganglioside GM1 on plasma membrane of sperm head and inhibits capacitation vitro as decapacitation factor. Furthermore, male mice lacking display prominently reduced fertility vivo, which indicates protects spermatozoa from some spermicidal attack uterus. In this study, we tried investigate mechanisms controls vivo...
Alzheimer's disease (AD) is a neurodegenerative disorder with cognitive impairment. Physical exercise has long been proven to be beneficial in the disorder. The present study was designed examine effect of voluntary on spatial memory, imaging, and pathological abnormalities. Particular focus given role heme oxygenase-1 (HO-1)-an important cellular cytoprotectant preserving mental acuity-using an aging rat model dementia. Male female Wistar rats were segregated into six groups-namely, (i)...
The purpose of this study was to develop a folate receptor-targeted 68Ga-labeled agent for the detection cancer cells in mouse models ovarian by dual positron-emission tomography (PET) and magnetic resonance imaging (MRI). Moreover, we aimed controlled biopolymer-based chemistry that enables linking metal-binding (here Ga-68) chelators.The nanoparticle (NP) created self-assembling folic acid-modified polyglutamic acid chelator-modified chitosan followed radiolabeling with 68Ga (III) ions...
A Zn II responsive Mn -based MRI contrast agent, [Mn(PC2A-DPA)], has been synthesized, investigated and applied in imaging studies. It shows high stability excellent inertness can be used to visualize glucose triggered release by MRI.
Today septins are considered as the fourth component of cytoskeleton, with Septin7 isoform playing a critical role in formation higher-order structures. While its importance has already been confirmed several intracellular processes different organs, very little is known about skeletal muscle. Here, using conditional knockdown (KD) mouse model, C2C12 cell line, and enzymatically isolated adult muscle fibers, organization localization septin filaments revealed, an ontogenesis-dependent...
Among humanized monoclonal antibodies, bevacizumab specifically binds to vascular endothelial growth factor A (VEGF-A). VEGF-A is an overexpressed biomarker in cervix carcinoma and involved the development maintenance of tumor-associated neo-angiogenesis. The non-invasive positron emission tomography using radiolabeled target-specific antibodies (immuno-PET) provides longitudinal quantitative assessment tumor target expression. Due having a long-circulating time, radioactive metal ions...
Dosage forms with increased gastric residence time are promising tools to increase bioavailability of drugs narrow absorption window. Low-density floating formulations could avoid emptying; therefore, sustained drug release can be achieved. Our aim was develop a new technology produce low-density by melt foaming. Excipients were selected carefully, the criteria low irritation, melting range below 70°C and well-known use in oral formulations. PEG 4000, Labrasol stearic acid type 50 used...
Time-lapse video microscopy was designed to follow the movement of single cells for an unlimited period time under physiological conditions. The system is based on two inverted microscopes located in a CO(2) incubator and equipped with charge-coupled device cameras connected computer. Frames were recorded every minute subsequent sequence converted database form. applied describe movements normal HaCaT Pb-treated causing so-called apoptotic dance during cell death. also followed...