A5043600016- Muscle Physiology and Disorders
- Ion channel regulation and function
- Adipose Tissue and Metabolism
- Ion Channels and Receptors
- Breast Cancer Treatment Studies
- Cardiomyopathy and Myosin Studies
- Genetic Neurodegenerative Diseases
- Osteoarthritis Treatment and Mechanisms
- Neuroscience and Neuropharmacology Research
- Exercise and Physiological Responses
- Advanced Radiotherapy Techniques
- Adenosine and Purinergic Signaling
- RNA Interference and Gene Delivery
- Neuropeptides and Animal Physiology
- Mitochondrial Function and Pathology
- Neurobiology and Insect Physiology Research
- Breast Lesions and Carcinomas
- Cannabis and Cannabinoid Research
- Neuroscience and Neural Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Antioxidant Activity and Oxidative Stress
- Cutaneous lymphoproliferative disorders research
- MicroRNA in disease regulation
- Radiation Therapy and Dosimetry
- Mast cells and histamine
University of Debrecen
2014-2024
National Institute of Oncology
1975-2022
Országos Reumatológiai és Fizioterápiás Intézet
1996-2021
Országos Pszichiátriai és Neurológiai Intézet
2004
National Institute of Criminology
1986
Abstract Ca 2+ ‐signaling of human melanoma is in the focus intensive research since identification role WNT‐signaling melanomagenesis. Genomic and functional studies pointed to important various channels melanoma, but these data were contradictory. In present study we clearly demonstrate, a number different ways including microarray analysis, DNA sequencing immunocytochemistry, that cell lines tissues overexpress ryanodine receptor type 2 (RyR2) express P2X 7 channel proteins as compared...
Abstract During aging reduction in muscle mass (sarcopenia) and decrease physical activity lead to partial loss of force increased fatigability. Deficiency the essential trace element selenium might augment these symptoms as it can cause pain, fatigue, proximal weakness. Average voluntary daily running, maximal twitch tetanic force, calcium release from sarcoplasmic reticulum (SR) decreased while reactive oxygen species (ROS) production associated with contractions aged – 22-month-old...
Understanding the key elements of signaling chondroprogenitor cells at earliest steps differentiation may substantially improve our opportunities for application mesenchymal stem in cartilage tissue engineering, which is a promising approach regenerative therapy joint diseases. Ion channels, membrane potential and Ca(2+)-signaling are important regulators cell proliferation differentiation. Our aim was to identify such plasma ion channels involved during chondrogenesis, serve as specific...
TASK-3 channel overexpression was shown to facilitate the survival of malignantly transformed cells, possibly by providing greater hypoxia tolerance through a still unknown mechanism. Although it has been suggested previously that channels are expressed in mitochondrial membranes, their role here remains elusive. In this study, transient transfection knockdown melanoma cell cultures produced show significance expression. Reduction protein biosynthesis induced characteristic changes...
Key points Marijuana was found to cause muscle weakness, although the exact regulatory role of its receptors (CB1 cannabinoid receptor; CB1R) in excitation–contraction coupling (ECC) mammalian skeletal remains unknown. We that CB1R activation or knockout did not affect force directly, whereas decreased Ca 2+ ‐sensitivity contractile apparatus and made fibres more prone fatigue. demonstrate CB1Rs are connected inositol 1,4,5‐trisphosphate pathway either myotubes adult fibres. By contrast,...
Key points Hypermuscularity associated with naturally occurring mutations in the myostatin gene as found Compact mice results increased muscle mass but reduced specific force. The calcium sensitivity of contractile apparatus assessed on chemically skinned skeletal fibres under isometric conditions is not altered these animals. While resting concentration remains unaffected, depolarization‐evoked increases intracellular are suppressed. Spontaneous release events from sarcoplasmic reticulum...
Today septins are considered as the fourth component of cytoskeleton, with Septin7 isoform playing a critical role in formation higher-order structures. While its importance has already been confirmed several intracellular processes different organs, very little is known about skeletal muscle. Here, using conditional knockdown (KD) mouse model, C2C12 cell line, and enzymatically isolated adult muscle fibers, organization localization septin filaments revealed, an ontogenesis-dependent...
The 95 kDa triadin (Trisk 95), an integral protein of the sarcoplasmic reticular membrane in skeletal muscle, interacts with both ryanodine receptor (RyR) and calsequestrin. While its role regulation calcium homeostasis has been extensively studied, data are not available on whether overexpression or interference expression Trisk would affect sparks localized events release (LCRE). In present study LCRE transients were studied using laser scanning confocal microscopy C2C12 cells primary...
The sarcoplasmic/endoplasmic reticulum Ca2+ATPases (SERCAs) are the main Ca2+ pumps which decrease intracellular level by reaccumulating into sarcoplasmic reticulum. neonatal SERCA1b is major pump in myotubes and young muscle fibers. To understand its role during skeletal differentiation synthesis has been interfered with specific shRNA sequence. Stably transfected clones showing significantly decreased expression (cloneC1) were selected for experiments. of regulatory proteins was examined...
Obscurin is a giant sarcomeric protein expressed in striated muscles known to establish several interactions with other proteins of the sarcomere, but also sarcoplasmic reticulum and costameres. Here, we report experiments aiming better understand contribution obscurin skeletal muscle fibers, starting detailed characterization diaphragm function, which previously reported be most affected (
The endocannabinoid system (ECS) refers to a widespread signaling and its alteration is implicated in growing number of human diseases. Cannabinoid receptors (CBRs) are highly expressed the central nervous many peripheral tissues. Evidence suggests that CB1Rs murine skeletal muscle mainly cell membrane, but subpopulation present also mitochondria. However, very little known about latter population. To date, connection between function regulation intracellular Ca2+ has not been investigated...
Abstract Background In vitro chondrogenesis depends on the concerted action of numerous signalling pathways, many which are sensitive to changes intracellular Ca 2+ concentration. N -methyl-D-aspartate (NMDA) glutamate receptor is a cation channel with high permeability for . Whilst there now accumulating evidence expression and function NMDA receptors in non-neural tissues including mature cartilage bone, contribution regulation yet be elucidated. Methods We studied role glutamatergic...