A5061621955- Osteoarthritis Treatment and Mechanisms
- Neuropeptides and Animal Physiology
- Signaling Pathways in Disease
- Silk-based biomaterials and applications
- Neurobiology and Insect Physiology Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Circadian rhythm and melatonin
- Hypothalamic control of reproductive hormones
- Cancer-related molecular mechanisms research
- Cancer, Stress, Anesthesia, and Immune Response
- Antimicrobial Peptides and Activities
- RNA Research and Splicing
- Proteoglycans and glycosaminoglycans research
- Adenosine and Purinergic Signaling
- Ion Channels and Receptors
- Cell Adhesion Molecules Research
- Receptor Mechanisms and Signaling
- RNA Interference and Gene Delivery
- Ion channel regulation and function
- Cancer-related gene regulation
- Inflammatory mediators and NSAID effects
- Pain Mechanisms and Treatments
- Bone and Dental Protein Studies
- Knee injuries and reconstruction techniques
- NF-κB Signaling Pathways
University of Debrecen
2015-2024
Bone graft substitutes and bone void fillers are predominantly used to treat defects fusion in orthopaedic surgery. Some aragonite-based scaffolds of coralline exoskeleton origin exhibit osteoconductive properties described as useful repair scaffolds. The purpose this study was evaluate the vitro osteogenic potential phase a novel bi-phasic osteochondral scaffold (Agili-C™, CartiHeal Ltd.) using adult human marrow-derived mesenchymal stem cells (MSCs). Analyses were performed at several time...
Pituitary adenylate cyclase activating polypeptide (PACAP) is an important neurotrophic factor influencing differentiation of neuronal elements and exerting protecting role during traumatic injuries or inflammatory processes the central nervous system. Although increasing evidence available on its presence function in various peripheral tissues, little known about PACAP formation skeletal components. To this end, we aimed to map signalling developing cartilage under physiological conditions...
Abstract Chondrogenesis is a multistep process, in which cartilage progenitor cells generate tissue with distinct structural and functional properties. Although several approaches to regeneration rely on the differentiation of implanted cells, temporal transcriptomic landscape vitro chondrogenesis different models has not been reported. Using RNA sequencing, we examined differences gene expression patterns during formation micromass cultures embryonic limb bud-derived progenitors. Principal...
Understanding the key elements of signaling chondroprogenitor cells at earliest steps differentiation may substantially improve our opportunities for application mesenchymal stem in cartilage tissue engineering, which is a promising approach regenerative therapy joint diseases. Ion channels, membrane potential and Ca(2+)-signaling are important regulators cell proliferation differentiation. Our aim was to identify such plasma ion channels involved during chondrogenesis, serve as specific...
Mature and developing chondrocytes exist in a microenvironment where mechanical load, changes of temperature, osmolarity acidic pH may influence cellular metabolism. Polymodal Transient Receptor Potential Vanilloid (TRPV) receptors are environmental sensors mediating responses through activation linked intracellular signalling pathways. In chondrogenic high density cultures established from limb buds chicken mouse embryos, we identified TRPV1, TRPV2, TRPV3, TRPV4 TRPV6 mRNA expression with...
Murine micromass models have been extensively applied to study chondrogenesis and osteogenesis elucidate pathways of endochondral bone formation. Here we provide a detailed comparative analysis the differentiation potential cultures established from either BMP-2 overexpressing C3H10T1/2 cells or mouse embryonic limb bud-derived chondroprogenitor cells, using untransfected as controls. Although failed form chondrogenic nodules, both expressed mRNA transcripts for major cartilage-specific...
Abstract Semicarbazide-sensitive amine oxidase (SSAO) catalyses oxidative deamination of primary amines. Since there is no data about its function in pain and arthritis mechanisms, we investigated the effects our novel SSAO inhibitor SzV-1287 chronic mouse models joint inflammation. Effects (20 mg/kg i.p./day) were K/BxN serum-transfer complete Freund’s adjuvant (CFA)-evoked active immunization compared to reference LJP-1207. Mechanonociception was assessed by aesthesiometry, oedema...
Pituitary adenylate cyclase activating polypeptide (PACAP) is an endogenous neuropeptide also secreted by non-neural cells, including chondrocytes. PACAP signaling involved in the regulation of chondrogenesis, but little known about its connection to matrix turnover during cartilage formation and under cellular stress developing cartilage. We found that expression activity hyaluronidases (Hyals), metalloproteinases (MMP), aggrecanase were permanent course chondrogenesis primary chicken...
The biomechanical environment plays a key role in regulating cartilage formation, but the current understanding of mechanotransduction pathways chondrogenic cells is incomplete. Among combination external factors that control chondrogenesis are temporal cues governed by cell-autonomous circadian clock. However, mechanical stimulation has not yet directly been proven to modulate via entraining clock chondroprogenitor cells. purpose this study was establish whether stimuli entrain core cells,...
Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurohormone exerting protective function during various stress conditions either in mature or developing tissues. Previously we proved the presence of PACAP signaling elements chicken limb bud-derived chondrogenic cells micromass cell cultures. Since no data can be found if playing any role mechanical tissues, aimed to investigate its contribution mechanotransduction chondrogenesis. Expressions mRNAs and major receptor, PAC1...
PACAP is a neuropeptide with diverse functions in various organs, including reproductive system. It present the testis high concentrations, and addition to stage-specific expression within seminiferous tubules, affects spermatogenesis of Leydig Sertoli cells. Mice lacking endogenous show reduced fertility, but possibility abnormalities spermatogenic signaling has not yet been investigated. Therefore, we performed detailed morphological analysis spermatozoa, sperm motility investigated...