A5015395421- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Muscle Physiology and Disorders
- Ion Channels and Receptors
- Ion channel regulation and function
- Lower Extremity Biomechanics and Pathologies
- Total Knee Arthroplasty Outcomes
- Neurobiology and Insect Physiology Research
- Adipose Tissue and Metabolism
- Bone and Joint Diseases
- Exercise and Physiological Responses
- Cardiomyopathy and Myosin Studies
- Periodontal Regeneration and Treatments
- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Genetic Neurodegenerative Diseases
- Tendon Structure and Treatment
- RNA Interference and Gene Delivery
- Muscle activation and electromyography studies
- Sleep and Wakefulness Research
- Hip disorders and treatments
- Virus-based gene therapy research
- Adenosine and Purinergic Signaling
- Pain Management and Treatment
- Veterinary Equine Medical Research
University of Debrecen
2013-2024
Saarland University
2018-2024
Abstract Accumulating evidence supports the role of astrocytes in endocannabinoid mediated modulation neural activity. It has been reported that some express cannabinoid type 1 receptor (CB -R), activation which is leading to Ca 2+ mobilization from internal stores and a consecutive release glutamate. also documented have potential produce 2-arachidonoylglycerol, one best known CB -R agonist. However, no relationship between 2-arachidonoylglycerol production ever demonstrated. Here we show...
Abstract Osteoarthritis (OA) is characterized by critical alterations of the subchondral bone microstructure, besides well‐known cartilaginous changes. Clinical computed tomography (CT) detection quantitative 3D microstructural parameters applied to monitor changes structure in different stages human OA and compared with micro‐CT, gold standard. Determination clinical CT (287 µm resolution) key tibial plateaus mild‐to‐moderate severe reveals strong correlations micro‐CT (35 µm), high inter‐...
Early osteoarthritis induced in a clinically relevant ovine model progresses highly specific spatiotemporal pattern resembling human OA.
Abstract During aging reduction in muscle mass (sarcopenia) and decrease physical activity lead to partial loss of force increased fatigability. Deficiency the essential trace element selenium might augment these symptoms as it can cause pain, fatigue, proximal weakness. Average voluntary daily running, maximal twitch tetanic force, calcium release from sarcoplasmic reticulum (SR) decreased while reactive oxygen species (ROS) production associated with contractions aged – 22-month-old...
Understanding the key elements of signaling chondroprogenitor cells at earliest steps differentiation may substantially improve our opportunities for application mesenchymal stem in cartilage tissue engineering, which is a promising approach regenerative therapy joint diseases. Ion channels, membrane potential and Ca(2+)-signaling are important regulators cell proliferation differentiation. Our aim was to identify such plasma ion channels involved during chondrogenesis, serve as specific...
As an essential trace element selenium plays a significant role in many physiological functions of the organs. It is found within muscles as selenocystein selenoprotein N, which involved redox-modulated calcium homeostasis and protection against oxidative stress.The effects two different compounds (selenate NanoSe 0.5 5 ppm concentration for weeks) on muscle properties mice were examined by measuring vivo performance, vitro force soleus (SOL) extensor digitorum longus (EDL) changes...
Key points Marijuana was found to cause muscle weakness, although the exact regulatory role of its receptors (CB1 cannabinoid receptor; CB1R) in excitation–contraction coupling (ECC) mammalian skeletal remains unknown. We that CB1R activation or knockout did not affect force directly, whereas decreased Ca 2+ ‐sensitivity contractile apparatus and made fibres more prone fatigue. demonstrate CB1Rs are connected inositol 1,4,5‐trisphosphate pathway either myotubes adult fibres. By contrast,...
Although osteoarthritis (OA), a leading cause of disability, has been associated with joint malalignment, scientific translational evidence for this link is lacking. In clinical case study, we provide osteochondral recovery upon unloading symptomatic isolated medial tibiofemoral knee OA varus malalignment. By mapping response correlations at high resolution, identify spatially complex degenerative changes in cartilage after overloading clinically relevant ovine model. We further report that...
Key points Hypermuscularity associated with naturally occurring mutations in the myostatin gene as found Compact mice results increased muscle mass but reduced specific force. The calcium sensitivity of contractile apparatus assessed on chemically skinned skeletal fibres under isometric conditions is not altered these animals. While resting concentration remains unaffected, depolarization‐evoked increases intracellular are suppressed. Spontaneous release events from sarcoplasmic reticulum...
Advanced human varus knee osteoarthritis correlates in a location-dependent and site-specific fashion with axial malalignment of the lower limb.
The 95 kDa triadin (Trisk 95), an integral protein of the sarcoplasmic reticular membrane in skeletal muscle, interacts with both ryanodine receptor (RyR) and calsequestrin. While its role regulation calcium homeostasis has been extensively studied, data are not available on whether overexpression or interference expression Trisk would affect sparks localized events release (LCRE). In present study LCRE transients were studied using laser scanning confocal microscopy C2C12 cells primary...
The sarcoplasmic/endoplasmic reticulum Ca2+ATPases (SERCAs) are the main Ca2+ pumps which decrease intracellular level by reaccumulating into sarcoplasmic reticulum. neonatal SERCA1b is major pump in myotubes and young muscle fibers. To understand its role during skeletal differentiation synthesis has been interfered with specific shRNA sequence. Stably transfected clones showing significantly decreased expression (cloneC1) were selected for experiments. of regulatory proteins was examined...
Obscurin is a giant sarcomeric protein expressed in striated muscles known to establish several interactions with other proteins of the sarcomere, but also sarcoplasmic reticulum and costameres. Here, we report experiments aiming better understand contribution obscurin skeletal muscle fibers, starting detailed characterization diaphragm function, which previously reported be most affected (
Articular cartilage defects represent an unsolved clinical challenge. Photopolymerizable hydrogels are attractive candidates supporting repair. This study investigates the short-term safety and efficacy of two novel hyaluronic acid (HA)-triethylene glycol (TEG)-coumarin photocrosslinked in situ a clinically relevant large animal model. It is hypothesized that HA-hydrogel-augmented microfracture (MFX) superior to MFX enhancing early repair, molar degree substitution concentration HA affects...
Abstract Background In vitro chondrogenesis depends on the concerted action of numerous signalling pathways, many which are sensitive to changes intracellular Ca 2+ concentration. N -methyl-D-aspartate (NMDA) glutamate receptor is a cation channel with high permeability for . Whilst there now accumulating evidence expression and function NMDA receptors in non-neural tissues including mature cartilage bone, contribution regulation yet be elucidated. Methods We studied role glutamatergic...
Abstract Selecting identical topographical locations to analyse pathological structural changes of the osteochondral unit in translational models remains difficult. The specific aim study was provide objectively defined reference points on ovine tibial plateau based 2-D sections micro-CT images useful for reproducible sample harvesting and as standardized landmarks landmark-based 3-D image registration. We propose 5 points, 11 lines 12 subregions that are detectable macroscopically sections....