A5076130380- Osteoarthritis Treatment and Mechanisms
- Circadian rhythm and melatonin
- Neuropeptides and Animal Physiology
- Cancer-related molecular mechanisms research
- Neurobiology and Insect Physiology Research
- Spaceflight effects on biology
- Silk-based biomaterials and applications
- Ion Channels and Receptors
- Adenosine and Purinergic Signaling
- Antimicrobial Peptides and Activities
- Cancer-related gene regulation
- RNA Research and Splicing
- Pain Mechanisms and Treatments
- Healthcare and Venom Research
- Proteoglycans and glycosaminoglycans research
- Postharvest Quality and Shelf Life Management
- Knee injuries and reconstruction techniques
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Hypothalamic control of reproductive hormones
- Vitamin K Research Studies
- Inflammasome and immune disorders
- Exercise and Physiological Responses
- Axon Guidance and Neuronal Signaling
- Chemokine receptors and signaling
- Signaling Pathways in Disease
University of Debrecen
2013-2024
Pituitary adenylate cyclase activating polypeptide (PACAP) is an important neurotrophic factor influencing differentiation of neuronal elements and exerting protecting role during traumatic injuries or inflammatory processes the central nervous system. Although increasing evidence available on its presence function in various peripheral tissues, little known about PACAP formation skeletal components. To this end, we aimed to map signalling developing cartilage under physiological conditions...
Abstract Chondrogenesis is a multistep process, in which cartilage progenitor cells generate tissue with distinct structural and functional properties. Although several approaches to regeneration rely on the differentiation of implanted cells, temporal transcriptomic landscape vitro chondrogenesis different models has not been reported. Using RNA sequencing, we examined differences gene expression patterns during formation micromass cultures embryonic limb bud-derived progenitors. Principal...
Mature and developing chondrocytes exist in a microenvironment where mechanical load, changes of temperature, osmolarity acidic pH may influence cellular metabolism. Polymodal Transient Receptor Potential Vanilloid (TRPV) receptors are environmental sensors mediating responses through activation linked intracellular signalling pathways. In chondrogenic high density cultures established from limb buds chicken mouse embryos, we identified TRPV1, TRPV2, TRPV3, TRPV4 TRPV6 mRNA expression with...
Murine micromass models have been extensively applied to study chondrogenesis and osteogenesis elucidate pathways of endochondral bone formation. Here we provide a detailed comparative analysis the differentiation potential cultures established from either BMP-2 overexpressing C3H10T1/2 cells or mouse embryonic limb bud-derived chondroprogenitor cells, using untransfected as controls. Although failed form chondrogenic nodules, both expressed mRNA transcripts for major cartilage-specific...
The biomechanical environment plays a key role in regulating cartilage formation, but the current understanding of mechanotransduction pathways chondrogenic cells is incomplete. Among combination external factors that control chondrogenesis are temporal cues governed by cell-autonomous circadian clock. However, mechanical stimulation has not yet directly been proven to modulate via entraining clock chondroprogenitor cells. purpose this study was establish whether stimuli entrain core cells,...
Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurohormone exerting protective function during various stress conditions either in mature or developing tissues. Previously we proved the presence of PACAP signaling elements chicken limb bud-derived chondrogenic cells micromass cell cultures. Since no data can be found if playing any role mechanical tissues, aimed to investigate its contribution mechanotransduction chondrogenesis. Expressions mRNAs and major receptor, PAC1...
Intense inflammation may result in pain, which manifests as spinal central sensitization. There is growing evidence that purinergic signaling plays a pivotal role the orchestration of pain processing. Over last decade ionotropic P2X purino receptor 4 (P2X4) got into spotlight neuropathic disorders, however its precise expression was scantily characterized during inflammatory pain. Thus, we intended to analyze distribution within dorsal horn and lumbar root ganglia (DRG) rats suffering...
Objective Circadian rhythms in cartilage homeostasis are hypothesized to temporally segregate and synchronize the activities of chondrocytes different times day, thus may provide an efficient mechanism by which articular can recover following physical activity. While circadian clock is clearly involved chondrocyte health disease, it unclear as what roles play during early chondrogenesis. Design The purpose this study was determine whether rhythmic expression core detectable at earliest...
Articular cartilage is characterized by a low density of chondrocytes surrounded an abundant extracellular matrix (ECM) consisting dense mixture collagens, proteoglycans, and glycosaminoglycans. Due to its cellularity high proteoglycan content, it particularly challenging extract high-quality total RNA suitable for sensitive high-throughput downstream applications such as sequencing (RNA-Seq). Available protocols isolation from articular are inconsistent, resulting in suboptimal yield...
Abstract Background In vitro chondrogenesis depends on the concerted action of numerous signalling pathways, many which are sensitive to changes intracellular Ca 2+ concentration. N -methyl-D-aspartate (NMDA) glutamate receptor is a cation channel with high permeability for . Whilst there now accumulating evidence expression and function NMDA receptors in non-neural tissues including mature cartilage bone, contribution regulation yet be elucidated. Methods We studied role glutamatergic...
Introduction Clusterin is a moonlighting protein that has many functions. It multifunctional holdase chaperone glycoprotein present intracellularly and extracellularly in almost all bodily fluids. involved lipid transport, cell differentiation, regulation of apoptosis, clearance cellular debris, plays protective role ensuring survival. However, the possible involvement clusterin arthritic disease remains unclear. Given significant potential as biomarker osteoarthritis (OA), more detailed...
Abstract Much of the skeletal system develops by endochondral ossification, a process that takes place in early fetal life. This makes stages chondrogenesis, i.e., when chondroprogenitor mesenchymal cells differentiate to chondroblasts, challenging study vivo. In vitro methods for chondrogenic differentiation have been available some time. There is currently high interest developing fine‐tuned methodology would allow rebuild articular cartilage and restore joint functionality. The micromass...
Abstract Embryonic limb bud‐derived micromass cultures are valuable tools for investigating cartilage development, tissue engineering, and therapeutic strategies cartilage‐related disorders. This collection of fine‐tuned protocols used in our laboratories outlines step‐by‐step procedures the isolation, expansion, differentiation primary mouse bud cells into chondrogenic cultures. Key aspects covered these include synchronized fertilization mice (Basic Protocol 1), dissection, cell formation,...