A5083684200- Osteoarthritis Treatment and Mechanisms
- Virus-based gene therapy research
- Knee injuries and reconstruction techniques
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Periodontal Regeneration and Treatments
- Tendon Structure and Treatment
- Cancer-related molecular mechanisms research
- Proteoglycans and glycosaminoglycans research
- Silk-based biomaterials and applications
- Total Knee Arthroplasty Outcomes
- Cell Adhesion Molecules Research
- interferon and immune responses
- Electrospun Nanofibers in Biomedical Applications
- Retinal Development and Disorders
- Lower Extremity Biomechanics and Pathologies
- Viral Infections and Immunology Research
- Inflammatory mediators and NSAID effects
- Chemokine receptors and signaling
- Metabolism and Genetic Disorders
- Orthopedic Infections and Treatments
- Graphene and Nanomaterials Applications
- Polymer Surface Interaction Studies
- Photoreceptor and optogenetics research
- Tissue Engineering and Regenerative Medicine
Saarland University
2015-2024
Université Sorbonne Paris Nord
2021
Sorbonne Université
2021
University of Washington
2019
Advanced biomaterial-guided delivery of gene vectors is an emerging and highly attractive therapeutic solution for targeted articular cartilage repair, allowing a controlled minimally invasive in spatiotemporally precise manner, reducing intra-articular vector spread possible loss the product. As far as it known, very first successful vivo application such potent orthotopic large animal model damage reported here. In detail, injectable thermosensitive hydrogel based on poly(ethylene oxide)...
Abstract Introduction Transplantation of genetically modified human bone marrow-derived mesenchymal stem cells (hMSCs) with an accurate potential for chondrogenic differentiation may be a powerful means to enhance the healing articular cartilage lesions in patients. Here, we evaluated benefits delivering SOX9 (a key regulator chondrocyte and formation) via safe, maintained, replication-defective recombinant adeno-associated virus (rAAV) vector on capability hMSCs commit adequate phenotype...
Abstract The regeneration of focal articular cartilage defects is complicated by the reduced quality repair tissue and potential development perifocal osteoarthritis (OA). Biomaterial‐guided gene therapy may enhance controlling release therapeutic sequences in a spatiotemporal manner. Here, benefits delivering recombinant adeno‐associated virus (rAAV) vector coding for human insulin‐like growth factor I (IGF‐I) via an alginate hydrogel (IGF‐I/AlgPH155) to full‐thickness chondral following...
Administration of therapeutic genes to human osteoarthritic (OA) cartilage is a potential approach generate effective, durable treatments against this slow, progressive disorder. Here, we tested the ability recombinant adeno-associated virus (rAAV)-mediated overexpression insulinlike growth factor (hIGF)-I reproduce an original surface in OA light pleiotropic activities factor. We examined proliferative, survival and anabolic effects rAAV-hIGF-I treatment primary normal chondrocytes vitro...
Abstract Background Therapeutic gene transfer is of significant value to elaborate efficient, durable treatments against human osteoarthritis (OA), a slow, progressive, and irreversible disorder for which there no cure date. Methods Here, we directly applied recombinant adeno-associated virus (rAAV) vector carrying transforming growth factor beta (TGF-β) sequence primary normal OA chondrocytes in vitro cartilage explants situ monitor the stability transgene expression effects candidate...
Genetic modification of bone marrow-derived mesenchymal stem cells (MSCs) for use in transplantation settings may be a valuable strategy to enhance the repair processes articular cartilage defects. Here, we evaluated potential overexpressing transforming growth factor (TGF)-β via recombinant adeno-associated viral (rAAV) vector-mediated gene transfer promote chondrogenic differentiation human MSCs (hMSCs). A TGF-β sequence was delivered undifferentiated and chondrogenically induced primary...
The transplantation of genetically modified progenitor cells such as bone marrow-derived mesenchymal stem (MSCs) is an attractive strategy to improve the natural healing articular cartilage defects. In present study, we examined potential benefits sustained overexpression mitogenic and pro-anabolic insulin-like growth factor I (IGF-I) via gene transfer upon biological activities human MSCs (hMSCs).Recombinant adeno-associated vectors (rAAV) were used deliver a IGF-I coding sequence in...
Gene therapy is an attractive strategy for the durable treatment of human osteoarthritis (OA), a gradual, irreversible joint disease. carriers based on small adeno-associated virus (AAV) exhibit major efficacy in modifying damaged articular cartilage situ over extended periods time. Yet, clinical application recombinant AAV (rAAV) vectors remains complicated by presence neutralizing antibodies against viral capsid elements majority patients. The goal this study was to evaluate feasibility...
Recombinant adeno-associated virus (rAAV) vectors are well suited carriers to provide durable treatments for human osteoarthritis (OA). Controlled release of rAAV from polymeric micelles was already shown increase both the stability and bioactivity while overcoming barriers, precluding effective gene transfer. In present study, we examined convenience delivering via poly(ethylene oxide) (PEO) poly(propylene (PPO) (PEO–PPO–PEO) transfer overexpress transcription factor SOX9 in monolayers OA...
Recombinant adeno-associated virus (rAAV) vectors are clinically adapted to durably treat human osteoarthritis (OA). Controlled delivery of rAAV via polymeric micelles was reported enhance the temporal and spatial presentation into their targets. Here, we tested feasibility delivering poly (ethylene oxide) (PEO) (propylene (PPO) (poloxamer poloxamine) as a means overexpress therapeutic factor transforming growth factor-beta (TGF-β) in OA chondrocytes experimental osteochondral defects....
Application of the chondrogenic transforming growth factor beta (TGF-β) is an attractive approach to enhance intrinsic biological activities in damaged articular cartilage, especially when using direct gene transfer strategies based on clinically relevant recombinant adeno-associated viral (rAAV) vectors.To evaluate ability rAAV-TGF-β construct modulate early repair processes sites focal cartilage injury minipigs vivo relative control (reporter lacZ gene) vector treatment.Controlled...
Abstract Background Articular cartilage has a limited potential for self-healing. Transplantation of genetically modified progenitor cells like bone marrow-derived mesenchymal stem (MSCs) is an attractive strategy to improve the intrinsic repair capacities damaged articular cartilage. Methods In this study, we examined benefits co-overexpressing pleiotropic transformation growth factor beta (TGF-β) with cartilage-specific transcription SOX9 via gene transfer recombinant adeno-associated...
Background: Osteochondral defects, if left untreated, do not heal and can potentially progress toward osteoarthritis. Direct gene transfer of basic fibroblast growth factor 2 (FGF-2) with the clinically adapted recombinant adeno-associated viral (rAAV) vectors is a powerful tool to durably activate osteochondral repair processes. Purpose: To examine ability an rAAV-FGF-2 construct target healing processes focal injury over time in large translational model vivo versus control condition....
Controlled release of TGF-β1 from scaffolds is an attractive mechanism to modulate the chondrogenesis human bone marrow mesenchymal stem cells (hBMSCs) that repopulate articular cartilage defects. Here, we evaluated ability porous composed poly(ethylene oxide)-terephtalate and poly(butylene terepthalate) (PEOT/PBT) bioactive for hBMSCs in a pellet culture model. Chondroinduction was compared with promoted by direct addition recombinant factor medium. The data show controlled at least 21 days...