A5089730864- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Proteoglycans and glycosaminoglycans research
- Growth Hormone and Insulin-like Growth Factors
- Total Knee Arthroplasty Outcomes
- Lower Extremity Biomechanics and Pathologies
- Cell Adhesion Molecules Research
- Inflammatory mediators and NSAID effects
- Silk-based biomaterials and applications
- Tendon Structure and Treatment
- Periodontal Regeneration and Treatments
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Hip disorders and treatments
- Mesenchymal stem cell research
- Musculoskeletal Disorders and Rehabilitation
- TGF-β signaling in diseases
- Spine and Intervertebral Disc Pathology
- Fibroblast Growth Factor Research
- Viral Infectious Diseases and Gene Expression in Insects
- Bone fractures and treatments
- Marriage and Sexual Relationships
- Bone Metabolism and Diseases
- Ethics in Clinical Research
- Bone and Joint Diseases
Indiana University
2003-2025
Indiana University School of Medicine
2012-2023
Indiana University – Purdue University Indianapolis
2012-2023
University School
2005-2022
Richard L. Roudebush VA Medical Center
2017
Massachusetts General Hospital
1995-2007
Orthopaedic Research Laboratories
1995-2007
Harvard University
1990-2007
Boston University
1996-1999
Biomed Research Institute
1994
A prospective case-control study of Lassa fever was established in Sierra Leone to measure the frequency and case-fatality ratio among febrile hospital admissions better delineate clinical diagnosis course this disease. responsible for 10%–16% all adult medical rv30% deaths two hospitals studied. The 441 hospitalized patients 16.5%. We found best predictor be combination fever, pharyngitis, retrosternal pain, proteinuria (predictive value together, .81); outcome, sore throat, vomiting...
Articular cartilage is routinely subjected to mechanical forces and cell-regulatory molecules. Previous studies have shown that stimuli can influence articular chondrocyte metabolic activity, biochemical growth factors cytokines control many of the same cell functions. Little known, however, relationships or interplay, if any, between these two key components environment. This study investigated comparative interactive effects low amplitude, sinusoidal, dynamic compression insulin-like...
Abstract We have examined the effect of peptide growth factors on DNA and proteoglycan synthesis by adult bovine articular cartilage in organ culture. The actions somatomedin‐C/insulin‐like factor I (Sm‐C/IGF‐I), insulin, epidermal (EGF), fibroblast (FGF) from pituitary were investigated individually combination. FGF stimulated a 10‐fold increase tritiated thymidine incorporation while other used did not influence mitotic activity. Used concert, insulin with EGF acted synergistically...
Abstract The in vivo measurement of articular cartilage deformation is essential to understand how mechanical forces distribute throughout the healthy tissue and change over time pathologic joint. Displacements or strain may serve as a functional imaging biomarker for healthy, diseased repaired tissues, but unfortunately intratissue largely unknown. Here, we directly quantified first patterns through thickness tibiofemoral human volunteers. Magnetic resonance acquisitions were synchronized...
Therapeutic gene transfer into articular cartilage is a potential means to stimulate reparative activities in tissue lesions. We previously demonstrated that direct application of recombinant adeno-associated virus (rAAV) vectors chondrocytes their native matrix situ as well sites damage allowed for efficient and sustained reporter expression. Here we test the hypothesis rAAV-mediated overexpression fibroblast growth factor 2 (FGF-2), one candidate enhancing repair lesions, would lead...
Successful gene transfer into articular cartilage is a prerequisite for therapy of joint disorders. In the present study we tested hypothesis that recombinant adeno-associated virus (rAAV) vectors are capable effecting in isolated chondrocytes vitro, tissue and sites damage vivo. Using an rAAV vector carrying Escherichia coli β-galactosidase (lacZ) under control cytomegalovirus (CMV) immediate-early promoter/enhancer (rAAV-lacZ), transduction efficiency exceeded 70% normal human adult...
A study was performed in order to investigate the possible functional roles of insulin-like growth-factor I (IGFI) and basic fibroblast growth factor (bFGF) regulation mitotic metabolic activity growth-plate chondrocytes. Chondrocytes from distal radial plates calves costal physeal cartilage rats were exposed these factors, individually combination, primary monolayer culture, assess their effects. The data showed that bFGF had both a greater potency efficacy as mitogen for bovine...
Articular chondrocytes respond to mechanical forces by alterations in gene expression, proliferative status, and metabolic functions. Little is known concerning the cell signaling systems that receive, transduce, convey information chondrocyte interior. Here, we show ex vivo cartilage compression stimulates phosphorylation of ERK1/2, p38 MAPK, SAPK/ERK kinase-1 (SEK1) JNK pathway. Mechanical induced a phased ERK consisting rapid induction ERK1/2 at 10 min, decay, sustained level ERK2...
Defects of articular cartilage are an unsolved problem in orthopaedics. In the present study, we tested hypothesis that gene transfer human fibroblast growth factor 2 (FGF-2) via transplantation encapsulated genetically modified chondrocytes stimulates chondrogenesis defects vivo.Lapine overexpressing a lacZ or FGF-2 sequence were alginate and further characterized. The resulting spheres applied to knee joints rabbits. vivo, repair was assessed qualitatively quantitatively at 3 14 weeks...
TRIPPEL, STEPHEN B. M.D.‡; COUTTS, RICHARD D. EINHORN, THOMAS A. MUNDY, GREGORY R. ROSENFELD, RON G. M.D.‡, BOSTON, MASSACHUSETTS Author Information
The interaction of somatomedin (Sm) with growth plate chondrocytes (GPCs) is believed to be the primary stimulus skeletal growth. Using techniques designed disrupt as little possible phenotypic characteristics GPCs, we have been able obtain 3–4 × 108 viable cells from major physes one newborn calf. availability these plus essentially pure Sm-C/insulin-like factor I, most GH-dependent Sm, has now made detailed studies this radiolabeled peptide GPC receptor and subsequent processing hormone by...
Chondrogenic polypeptide growth factors influence articular chondrocyte functions that are required for cartilage repair. Sox9 is a transcription factor regulates chondrogenesis, but its role in the regulation of proliferation and matrix synthesis poorly understood. We tested hypotheses selected chondrogenic regulate sox9 gene expression protein production by adult chondrocytes modulates actions these factors. To test hypotheses, we delivered insulin-like factor-I (IGF-I), fibroblast...
Traumatic knee injuries in humans trigger an immediate increase synovial fluid levels of inflammatory cytokines that accompany impact damage to joint tissues. We developed a human vitro cartilage-bone-synovium (CBS) coculture model study the role mechanical injury and inflammation initiation post-traumatic osteoarthritis (PTOA)-like disease.
Murray J. Favus, editor. Washington, DC: American Society for Bone and Mineral Research; 2003. 567 pages. $49.95. Like its predecessors, the latest edition of this now-classic reference book provides a comprehensive overview bone diseases disorders mineral metabolism in single volume. The title is something misnomer as it does not fully describe content book. Rather than being restricted to pathology, implied by title, also an excellent background biology normal bone. This has been...