A5065325280- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Osteoarthritis Treatment and Mechanisms
- Complement system in diseases
- Adenosine and Purinergic Signaling
- Urological Disorders and Treatments
- Cardiac Ischemia and Reperfusion
- HIV Research and Treatment
- Cell Adhesion Molecules Research
- Renal Transplantation Outcomes and Treatments
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Muscle Physiology and Disorders
- Knee injuries and reconstruction techniques
- Child Nutrition and Feeding Issues
- Blood Coagulation and Thrombosis Mechanisms
- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Bone Metabolism and Diseases
- Virus-based gene therapy research
- Neonatal Health and Biochemistry
- Biomedical Ethics and Regulation
University of Freiburg
2016-2024
Saigon University
2022
Institute of Neuroimmunology of the Slovak Academy of Sciences
2022
University of Michigan
2019
University of Tübingen
2008-2017
University Children's Hospital Tübingen
2011-2015
Brigham and Women's Hospital
2002-2008
Harvard University
2003-2008
University of Colorado Denver
2007-2008
University of Colorado Hospital
2007-2008
Extracellular adenosine, generated from extracellular nucleotides via ectonucleotidases, binds to specific receptors and provides cardioprotection ischemia reperfusion. In the present study, we studied ecto-enzymatic ATP/ADP-phosphohydrolysis by select members of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) family during myocardial ischemia.As a first step, used murine model in situ preconditioning performed pharmacological studies with polyoxometalate 1, potent E-NTPDase...
Abstract Complement activation plays an important role in local and remote tissue injury associated with gastrointestinal ischemia-reperfusion (GI/R). The of the classical lectin complement pathways GI/R was evaluated using C1q-deficient (C1q KO), MBL-A/C-deficient (MBL-null), factor 2- B-deficient (C2/fB wild-type (WT) mice. Gastrointestinal ischemia (20 min), followed by 3-h reperfusion, induced intestinal lung C1q KO WT mice, but not C2/fB Addition human C2 to mice significantly restored...
Abstract Intestinal ischemia/reperfusion injury (IR) is characterized by intermittent loss of perfusion to the gut, resulting in dramatic increases morbidity and mortality. Based on previous studies indicating an anti-inflammatory role for hypoxia-inducible factor (HIF)-1–elicited enhancement extracellular adenosine production via ecto-5′-nucleotidase (CD73) signaling through A2B receptor (A2BAR), we targeted HIF-1 during IR using pharmacological or genetic approaches. Initial with HIF...
Introduction The last decade has led to rapid developments and increased usage of computational tools at the single-cell level. However, our knowledge remains limited in how extracellular cues alter quantitative macrophage morphology such morphological changes can be used predict phenotype as well cytokine content Methods Using an artificial intelligence (AI) based approach, this study determined whether (i) accurate classification (ii) prediction intracellular IL-10 level was possible,...
Mannose-binding lectin (MBL) is present in human serum and plays an important role innate immunity by binding to carbohydrate on micro-organisms. Whereas the gp120/gp41 of immunodeficiency virus type 1 (HIV-1) contains numerous N-linked glycosylation sites many these contain high-mannose glycans which could interact with MBL, interaction between MBL primary isolates (PI) HIV-1 has not been studied. To determine if PI HIV bind a capture assay was developed incubated MBL-coated microtitre...
Abstract Gastrointestinal ischemia/reperfusion (IR) injury significantly contributes to the morbidity and mortality of critical illness. In this study, we hypothesized a protective role for extracellular adenosine signaling in intestinal IR injury. Initial profiling studies mucosal scrapings following murine demonstrated selective induction A2B receptor (A2BAR) transcript. Moreover, gene-targeted mice A2BAR showed more profound compared with controls. contrast, A2AAR−/− exhibited no...
Abstract Ischemia/reperfusion injury (IRI) of the liver is an important cause hepatic dysfunction. Ischemic preconditioning (IP) associated with adenosine-mediated tissue protection from subsequent IRI. Extracellular nucleotides (e.g., ATP) represent main source for extracellular adenosine. Therefore, we hypothesized that phosphohydrolysis ATP/ADP via ectonucleoside triphosphate diphosphohydrolase-1 (CD39), conversion to AMP, mediates IP-dependent protection. We found IP was significant...
Ischemia and reperfusion represent major mechanisms of tissue injury organ failure. The timing administration the duration action limit current treatment approaches using pharmacological agents. In this study, we have successfully developed a preemptive strategy for protection an adenoassociated vector system containing erythropoietin hypoxia response elements ischemia-regulated expression therapeutic gene human heme-oxygenase-1 (hHO-1). We demonstrate that single several weeks in advance...
Extracellular adenosine has been implicated as an innate antiinflammatory metabolite, particularly during conditions of limited oxygen availability such ischemia. Because extracellular generation is primarily produced via phosphohydrolysis from its precursor molecule adenosine-monophosphate (AMP) through the enzyme ecto-5'-nucleotidase (CD73), we examined contribution CD73-dependent production in modulation intestinal ischemia-reperfusion (IR) injury. Following transcriptional and...
Despite innovations in surgical interventions, treatment of cartilage injury osteoarthritic joints remains a challenge due to concomitant inflammation. Obstructing single dominant inflammatory cytokine has shown remarkable clinical benefits rheumatoid arthritis, and similar strategies are being suggested target pathways osteoarthritis (OA). Here, we describe the utility gelatin microspheres that responsive proteolytic enzymes typically expressed arthritic flares, resulting on-demand...
Introduction Little is known how inflammatory processes quantitatively affect chondrocyte morphology and single cell morphometric data could be used as a biological fingerprint of phenotype. Methods We investigated whether trainable high-throughput quantitative profiling combined with population-based gene expression analysis can to identify fingerprints that are discriminatory control vs. phenotypes. The shape large number chondrocytes isolated from bovine healthy human osteoarthritic (OA)...
Background New daily persistent headache (NDPH) is a poorly understood disorder which often begins after viral infection and has been hypothesised to have an immune basis. Several studies found differences in peripheral serum cytokine levels between healthy controls patients with other primary disorders such as migraine. We sought measure of cytokines chemokines NDPH, chronic migraine (CM), controls, identify any changes could be biomarkers inflammation NDPH. Methods performed observational...
Objective— Adenosine signaling is known to inhibit platelet aggregation. Extracellular adenosine mainly stems from enzymatic phosphohydrolysis of precursor nucleotides via ecto-5′-nucleotidase. Previous studies suggest that soluble 5′-nucleotidase (5′-NT) derived Crotalus atrox venom may be clinically beneficial in vascular leakage, myocardial, renal, and intestinal ischemia, or acute lung injury. However, the effects 5′-NT treatment on aggregation remain unknown. We examined direct vivo ex...
Mesenchymal stromal cells (MSCs) are multipotent that can be differentiated in vitro into a variety of cell types, including adipocytes or osteoblasts. Our recent studies indicated high expression CD146 on MSCs from bone marrow correlates with their robust osteogenic differentiation potential. We therefore investigated if the placenta similar The were isolated specifically endometrial and fetal parts human term expanded separate cultures compared as controls. surface antigens was by flow...
Controlling mesenchymal stromal cell (MSC) shape is a novel method for investigating and directing MSC behaviour in vitro. it was hypothesized that specifigc shapes can be generated by using stiffness-defined biomaterial surfaces applying cyclic tensile forces. Biomaterials used were thin thick silicone sheets, fibronectin coating, compacted collagen type I sheets. The morphology quantified descriptors describing dimensions membrane protrusions. Nanoscale stiffness measured atomic force...