A5004608361- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive System and Pregnancy
- Cell Adhesion Molecules Research
- Extracellular vesicles in disease
- RNA Research and Splicing
- Tissue Engineering and Regenerative Medicine
- Bone Metabolism and Diseases
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- Mitochondrial Function and Pathology
- Assisted Reproductive Technology and Twin Pregnancy
- Congenital heart defects research
- Ovarian function and disorders
- Renal and related cancers
- Platelet Disorders and Treatments
- Metabolism and Genetic Disorders
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Caveolin-1 and cellular processes
- Cardiomyopathy and Myosin Studies
- Endometriosis Research and Treatment
- Animal Genetics and Reproduction
National Center For Child Health and Development
2016-2025
The University of Tokyo
2021
National Institutes of Health
2009-2014
Health and Human Development (2HD) Research Network
2008-2010
Tokyo Medical and Dental University
2007-2008
Osaka University
2000-2008
Japan Science and Technology Agency
2007
Kurume University
1998-2000
Kumamoto University
1999
Tokai University
1996-1998
CD9 is an integral membrane protein associated with integrins and other proteins. Mice lacking were produced by homologous recombination. Both male female CD9-/- mice born healthy grew normally. However, the litter size from females was less than 2% of that wild type. In vitro fertilization experiments indicated cause this infertility due to failure sperm-egg fusion. When sperm injected into oocytes assisted microfertilization techniques, however, fertilized eggs developed term. These...
Abstract Paracrine signaling by bone-marrow-derived mesenchymal stem cells (MSCs) plays a major role in tissue repair. Although the production of regulatory cytokines MSC transplantation is critical modulator regeneration, we focused on exosomes, which are extracellular vesicles that contain proteins and nucleic acids, as novel additional cell-to-cell communication regeneration. To address this, used radiologic imaging, histological examination, immunohistochemical analysis to evaluate...
Stem cell therapy can help repair damaged heart tissue. Yet many of the suitable cells currently identified for human use are difficult to obtain and involve invasive procedures. In our search novel stem with a higher cardiomyogenic potential than those available from bone marrow, we discovered that potent cardiac precursor-like be harvested menstrual blood. This represents new, noninvasive, source therapeutic material. We demonstrate blood-derived mesenchymal (MMCs) began beating...
What were the risks with regard to pregnancy outcomes of patients who conceived by frozen-thawed embryo transfer (FET) during a hormone replacement cycle (HRC-FET)?The HRC-FET had increased hypertensive disorders (HDP) and placenta accreta reduced risk gestational diabetes mellitus (GDM) in comparison those FET natural ovulatory (NC-FET).Previous studies have shown that live-birth rates after NC-FET are comparable. Little has been clarified regarding association between endometrium...
The heparin-binding epidermal growth factor (EGF)-like (HB-EGF) is a member of the EGF family factors that binds to and activates receptor (EGFR) related tyrosine kinase, ErbB4. HB-EGF-null mice (HB del/del ) were generated examine role HB-EGF in vivo . More than half HB died first postnatal week. survivors developed severe heart failure with grossly enlarged ventricular chambers. Echocardiographic examination showed chambers dilated cardiac function was diminished. Moreover, valves. valve...
Duchenne muscular dystrophy (DMD), the most common lethal genetic disorder in children, is an X-linked recessive muscle disease characterized by absence of dystrophin at sarcolemma fibers. We examined a putative endometrial progenitor obtained from tissue samples to determine whether these cells repair degeneration murine mdx model DMD. Implanted conferred human degenerated immunodeficient mice. then menstrual blood–derived primarily cultured nontransformed also dystrophied muscle. In vivo...
Tetraspanins CD9 and CD81 facilitate the fusion between gametes, myoblasts, or virus-infected cells. Here, we investigated role of these tetraspanins in mononuclear phagocytes. Expression their complex formation with integrins were up-regulated when blood monocytes cultured under normal conditions. Under fusogenic conditions presence Con A, up-regulation was inhibited, down-regulated. Anti-CD9 -CD81 antibodies, which previously shown to inhibit cells, unexpectedly promoted alveolar...
Membrane fusion is an essential step in the encounter of two nuclei from sex cells-sperm and egg-in fertilization. However, aside involvement molecules, CD9 Izumo, mechanism remains unclear. Here, we show that sperm-egg mediated by vesicles containing are released egg interact with sperm. We demonstrate CD9(-/-) eggs, which have a defective sperm-fusing ability, impaired release CD9-containing vesicles. investigate fusion-facilitating activity examining sperm to eggs aid exogenous Moreover,...
Rationale : Amniotic membrane is known to have the ability transdifferentiate into multiple organs and expected stimulate a reduced immunologic reaction. Objective Determine whether human amniotic membrane–derived mesenchymal cells (hAMCs) can be an ideal allograftable stem cell source for cardiac regenerative medicine. Methods Results We established hAMCs. After cardiomyogenic induction in vitro, hAMCs beat spontaneously, calculated transdifferentiation efficiency was 33%. Transplantation...
Significance Male mice lacking seminal vesicle secretion 2 (SVS2) protein, which is a major component of secretions, display prominently reduced fertility. However, their epididymal sperm are able to fertilize eggs normally in vitro, suggesting that SVS2 only essential for vivo fertilization. We demonstrate infertility −/− male caused not by failed copulatory plug formation but also the disruption ejaculated uterus uterus-derived cytotoxic factors. acts protect against these factors coating...
Abstract Background Oocytes may undergo two types of aging. The first is induced by exposure to an aged ovarian microenvironment before being ovulated, known as ‘reproductive or maternal aging’, and the second either a prolonged stay in oviduct fertilization vitro aging prior insemination, ‘postovulatory aging’. However, molecular mechanisms underlying these processes remain be elucidated. As telomere shortening cultured somatic cells triggers replicative senescence, oocytes during...
We tested the cardiomyogenic potential of human umbilical cord blood-derived mesenchymal stem cells (UCBMSCs). Both number and function may be depressed in senile patients with severe coronary risk factors. Therefore, obtained from such not well. For this reason, UCBMSCs are potentially a new cell source for cell-based therapy, since can younger populations being routinely utilized clinical patients. The (5 x 10(3) per cm(2)) were cocultured fetal murine cardiomyocytes ([CM] 1 10(5) cm(2))....
Mitochondria are abundant in fully grown mammalian oocytes with a unique spherical morphology, but the mechanisms controlling mitochondria behavior not well understood. Here we describe for first time control of mitochondrial mouse by fusion/fission mechanism. Mitofusins (Mfn1 and Mfn2) OPA1 proteins required outer inner membrane fusion, respectively, whereas Drp1 is key regulator fission. We show that express Mfn1, Mfn2, Opa1 proteins, both immature mature at similar levels. Overexpression...
CD9 and CD81 are closely related tetraspanins that regulate cell motility signaling by facilitating the organization of multimolecular membrane complexes, including integrins. We show down-regulated in smoking-related inflammatory response a macrophage line, RAW264.7. When functions were ablated with monoclonal antibody treatment, small interfering RNA transfection, or gene knock-out, macrophages less motile produced larger amounts matrix metalloproteinase (MMP)-2 MMP-9 than control cells...
Although sperm serine protease and proteasome have long been believed to play an important role in the fertilization process, molecular mechanism is still controversial. In this study, we produced double-knockout mice lacking two proteases, ACR PRSS21, uncover functional of trypsinlike activity fertilization. The male were subfertile, likely owing incompleteness oviductal ampulla. Despite subfertility, mutant epididymal exhibited inability undergo acrosomal exocytosis on zona pellucida (ZP)...
When a sperm and oocyte unite into one cell upon fertilization, membranous fusion between the occurs. In mice, Izumo1 tetraspanin molecule CD9 are required for sperm-oocyte as of factors, another CD81 is also thought to involve in this process. Since these two tetraspanins often form complex cell-cell interaction, it probable that such formed interaction; however, possibility still under debate among researchers. Here we assessed problem using mouse oocytes. Immunocytochemical analysis...
Cell membranes are composed of many different lipids and protein receptors, which important for regulating intracellular functions cell signaling. To orchestrate these activities, the membrane is compartmentalized into microdomains that stably or transiently formed. These compartments called “lipid rafts”. In gamete cells lack gene transcription, distribution proteins on lipid rafts focused during changes in their structure such as starting flagella movement fusion. this paper, we describe...
The oxidative modification of gametes by a reactive oxygen species is major deleterious factor that decreases the successful rate in vitro fertilization. Superoxide dismutase 1 (SOD1) plays pivotal role antioxidation scavenging superoxide anion, and its deficiency causes infertility female mice, but significance enzyme male mice remains unclear. In present study, we characterized Sod1(-/-) (Sod1-KO) reproductive organs compiled first report impaired fertilizing ability Sod1-KO sperm...