A5083111745- Cellular Mechanics and Interactions
- Skin and Cellular Biology Research
- Wound Healing and Treatments
- HER2/EGFR in Cancer Research
- Hair Growth and Disorders
- Pluripotent Stem Cells Research
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Corneal Surgery and Treatments
- Skin Protection and Aging
- 3D Printing in Biomedical Research
- Reproductive Biology and Fertility
- Monoclonal and Polyclonal Antibodies Research
- Cancer Cells and Metastasis
- Cancer and Skin Lesions
- Cancer-related gene regulation
- Retinoids in leukemia and cellular processes
- Animal Genetics and Reproduction
- Silk-based biomaterials and applications
- Optical Coherence Tomography Applications
- Telomeres, Telomerase, and Senescence
- Dermatologic Treatments and Research
- Ubiquitin and proteasome pathways
- Mesenchymal stem cell research
- Cardiac Valve Diseases and Treatments
The University of Tokyo
2021-2023
Tokyo University of Science
2023
École Polytechnique Fédérale de Lausanne
2010-2023
Tokyo Medical and Dental University
2015-2021
Ehime University
2005-2016
Ehime University Hospital
2015
University Hospital of Lausanne
2013
Osaka University
2000-2003
The heparin-binding epidermal growth factor (EGF)-like (HB-EGF) is a member of the EGF family factors that binds to and activates receptor (EGFR) related tyrosine kinase, ErbB4. HB-EGF-null mice (HB del/del ) were generated examine role HB-EGF in vivo . More than half HB died first postnatal week. survivors developed severe heart failure with grossly enlarged ventricular chambers. Echocardiographic examination showed chambers dilated cardiac function was diminished. Moreover, valves. valve...
Members of the epidermal growth factor (EGF) family are most important factors involved in epithelialization during cutaneous wound healing. Heparin-binding EGF-like (HB-EGF), a member EGF family, is thought to play an role skin To investigate vivo function HB-EGF healing, we generated keratinocyte-specific HB-EGF-deficient mice using Cre/loxP technology combination with keratin 5 promoter. Studies healing revealed that closure was markedly impaired mice. mRNA upregulated at migrating edge,...
Cleavage of membrane-anchored heparin-binding EGF-like growth factor (proHB-EGF) via metalloprotease activation yields amino- and carboxy-terminal regions (HB-EGF HB-EGF-C, respectively), with HB-EGF widely recognized as a key element epidermal receptor transactivation in G protein–coupled signaling. Here, we show biological role HB-EGF-C cells. Subsequent to proteolytic cleavage proHB-EGF, translocated from the plasma membrane into nucleus. This translocation triggered nuclear export...
Skin regenerative capacity declines with age, but the underlying mechanisms are largely unknown. Here we demonstrate a functional link between epidermal growth factor receptor (EGFR) signaling and type XVII collagen (COL17A1) proteolysis on age-associated alteration of keratinocyte stem cell dynamics in skin regeneration. Live-imaging computer simulation experiments predicted that human motility is coupled self-renewal Receptor tyrosine kinase array identified decline EGFR mouse wound...
A disintegrin and metalloprotease 12 (ADAM12/meltrin α) is a key enzyme implicated in the ectodomain shedding of membrane-anchored heparin-binding epidermal growth factor (EGF)-like (proHB-EGF)-dependent receptor (EGFR) transactivation. However, activation mechanisms ADAM12 are obscure. To determine how activated, we screened proteins that bind to cytoplasmic domain using yeast two-hybrid system identified protein called PACSIN3 contains Src homology 3 domain. An analysis interactions...
A disintegrin and metalloproteases (ADAMs) are implicated in the ectodomain shedding of epidermal growth factor receptor (EGFR) ligands EGFR transactivation. However, activation mechanisms ADAMs remain elusive. To analyze regulatory ADAM activation, we performed yeast two-hybrid screening using cytoplasmic domain ADAM12 as bait, identified a protein that designated Eve-1. Two cDNAs were cloned characterized. They encode alternatively spliced isoforms Eve-1, called Eve-1a Eve-1b, have four...
Image-based identification of cultured stem cells and noninvasive evaluation their proliferative capacity advance cell therapy research. Here we demonstrate that human keratinocyte can be identified in situ by analyzing motion during cultivation. Modeling experiments suggested the clonal type clonogenic keratinocytes efficiently determined analysis early movement. Image demonstrated indeed display a unique rotational movement as two-cell stage colony. We also α6 integrin is required for both...
Abstract Stem cell-based products have clinical and industrial applications. Thus, there is a need to develop quality control methods standardize stem cell manufacturing. Here, we report deep learning-based automated tracking (DeepACT) technology for noninvasive identification of cultured human cells. The combination cascading detection Kalman filter algorithm-based successfully tracked the individual cells within densely packed epidermal keratinocyte colonies in phase-contrast images...
Promyelocytic leukemia zinc finger (PLZF) protein, a transcriptional repressor and negative regulator of the cell cycle, has been characterized as prostatic androgen-responsive gene. DU145 cells show androgen-independent growth lack PLZF gene expression.We analyzed PLZF-regulating genes by DNA microarray using infected with LacZ- or PLZF-carrying adenoviruses.DNA revealed that Pbx1 is prominent suppressed in PLZF-overexpressing cells. Androgen receptor (AR)-expressing recovered...
Recently, neurotrophic factors and cytokines have been shown to be associated in psychiatric disorders, such as schizophrenia, bipolar disorder, depression. Heparin-binding epidermal growth factor-like factor (HB-EGF) is a member of the EGF family, serves molecular plays significant role brain. We generated mice which HB-EGF activity disrupted specifically ventral forebrain. These knockout showed (a) behavioral abnormalities similar those described were ameliorated by typical or atypical...
Previous studies have demonstrated that cell adhesion systems are downregulated in epithelial buds at the earliest stages of submandibular gland and hair follicle development, but restored subsequent stages. Here it is shown also remodeled during early mammary development. Immunofluorescence electron microscopy mouse bud cell–cell were hardly detectable, with significant downregulation expression desmosomal molecules, not E‐cadherin β‐catenin. Hemidesmosomal structures rarely found, although...
Remodeling of collagen fibrils is involved in a variety physiological and pathological processes including development, tissue repair, metastasis. Fibroblast-populated gel contraction has been employed as model system to investigate the fibril remodeling within three-dimensional matrices. Research on also important for understanding mechanism underlying connective design considerations engineered tissues regenerative medicine. Second harmonic generation (SHG) non-linier optical effect by...