A5042046866- Hormonal Regulation and Hypertension
- Cancer, Hypoxia, and Metabolism
- Pituitary Gland Disorders and Treatments
- Adrenal and Paraganglionic Tumors
- Estrogen and related hormone effects
- MicroRNA in disease regulation
- Adrenal Hormones and Disorders
- Drug Transport and Resistance Mechanisms
- Cancer-related molecular mechanisms research
- Stress Responses and Cortisol
- Metabolism and Genetic Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetic factors in colorectal cancer
- Chromatin Remodeling and Cancer
- Caveolin-1 and cellular processes
- Gut microbiota and health
- Hedgehog Signaling Pathway Studies
- Cancer Genomics and Diagnostics
- Cancer, Lipids, and Metabolism
- Genomics and Chromatin Dynamics
- Circular RNAs in diseases
- Sexual Differentiation and Disorders
- RNA Research and Splicing
- Cardiac tumors and thrombi
- Neuroendocrine Tumor Research Advances
Semmelweis University
2015-2025
Hungarian Research Network
2021-2024
University of Debrecen
2024
UD-GenoMed (Hungary)
2024
Institute of Molecular Life Sciences
2019-2024
HUN-REN Research Centre for Natural Sciences
2019-2024
Hungarian Academy of Sciences
2015-2023
National Institute of Oncology
2022-2023
University of Szeged
2016
Gedeon Richter (Hungary)
2006-2015
MicroRNAs (miRs) are involved in the pathogenesis of several neoplasms; however, there no data on their expression patterns and possible roles adrenocortical tumors. Our objective was to study tumors by an integrative bioinformatics analysis involving miR transcriptomics profiling, pathway analysis, a novel, tissue-specific target prediction approach. Thirty-six tissue samples including normal tissues, benign adenomas, carcinomas (ACC) were studied simultaneous mRNA profiling. A novel...
Whole transcriptome studies have become essential for understanding the complexity of genetic regulation. However, conventionally applied short-read sequencing platforms cannot be used to reliably distinguish between many transcript isoforms. The Pacific Biosciences (PacBio) RS II platform is capable reading long nucleic acid stretches in a single run. pseudorabies virus (PRV) an excellent system study herpesvirus gene expression and potential interactions transcriptional units. In this...
The tumorigenic mechanisms involved in pituitary adenomas, especially of nonfunctional adenomas (NFAs), remains unclear. Various cell cycle inhibitors have been found to be underexpressed tumors; however, Wee1 kinase, a nuclear protein that delays mitosis and was recently recognized as tumor suppressor gene, has not previously investigated tumors.Our objective examine the expression tumors identify microRNAs (miRs) can regulate its expression.Expression examined by immunohistochemistry...
Abstract Background Estrogens exert anti-inflammatory and neuroprotective effects in the brain mainly via estrogen receptors α (ERα) β (ERβ). These are members of nuclear receptor superfamily ligand-dependent transcription factors. This study was aimed at elucidation ERα ERβ agonists on expression neuroinflammatory genes frontal cortex aging female rats. Methods To identify estrogen-responsive immunity/inflammation genes, we treated middle-aged, ovariectomized rats with 17β-estradiol (E2),...
The intricate interactions between the immune, endocrine and central nervous systems shape innate immune response of brain. We have previously shown that estradiol suppresses expression genes in frontal cortex middle-aged ovariectomized rats, but not young ones reflecting elevated these middle-aged, ovarian hormone deficient animals. Here, we explored impact menopause on microglia phenotype capitalizing differential macrophage-associated quiescent activated microglia. selected twenty-three...
Abstract Multigene panel tests (MGPTs) revolutionized the diagnosis of Lynch syndrome (LS), however noncoding pathogenic variants (PVs) can only be detected by complementary methods including whole genome sequencing (WGS). Here we present a DNA-, RNA- and tumor tissue-based WGS prioritization workflow for patients with suspicion LS where MGPT no LS-related PV. Among 100 enrolled patients, 28 simple PVs an additional 3 complex PVs. 69 MGPT-negative lack somatic MLH1 promoter methylation in...
Background Developing chemotherapy resistant cell lines can help to identify markers of resistance. Instead using a panel highly heterogeneous lines, we assumed that truly robust and convergent pattern resistance be identified in multiple parallel engineered derivatives only few parental lines. Methods Parallel populations were initiated for two breast cancer (MDA-MB-231 MCF-7) these treated independently 18 months with doxorubicin or paclitaxel. IC50 values against 4 agents determined...
Estradiol (E2) modulates a wide range of functions the frontal cerebral cortex. From onset menopause, declining levels E2 can cause cognitive disturbances and changes in behavior that be counterbalanced by hormone replacement. To study effect replacement on cortical transcriptome rodent model with low serum level, we treated middle-aged, ovariectomized rats or vehicle using osmotic minipumps for 4 wk. Six animals each group were selected, samples their cortex subjected to expression...
Abstract Background Circulating miRNAs in pituitary adenomas would improve patient care, especially as minimally invasive biomarkers of tumor recurrence and progression nonfunctioning adenoma cases. Aim Our aim was to investigate plasma miRNA profiles patients with adenomas. Materials Methods A total 149 extracellular vesicle (preoperative, early postoperative, late postoperative) samples were collected from 45 Adenomas characterized on the basis anterior hormones transcription factors by...
Summary Objective The hypothalamic–pituitary–adrenal axis setpoint and the glucocorticoid sensitivity of various tissues are at least partially genetically determined. We investigated impact receptor (GR) gene polymorphisms, including BclI, N363S, ER22/23EK A3669G variants on bone turnover and/or mineral density (BMD) in patients with endogenous excess. Design Sixty 35 ACTH producing pituitary adenoma (CD) 25 adrenal Cushing's syndrome (ACS) as well 129 healthy subjects were genotyped....
Adrenal myelolipoma (AML) is the second most common and invariably benign primary adrenal neoplasm. Due to variable proportion of fat hematopoietic elements its often large size, it can cause differential diagnostic problems. Several reports confirmed utility miRNAs in diagnosis tumors, but miRNA expression AML has not yet been investigated. Next-generation sequencing (NGS) was performed on 30 formalin-fixed, paraffin-embedded (FFPE) archived tissue samples [10 each AML, adrenocortical...
Abstract Urbanization is rapidly shaping and transforming natural environments, creating networks of modified land types. These urbanization‐driven modifications lead to local extinctions several species, but the surviving ones also face numerous novel selection pressures, including exposure pollutants, habitat alteration, shifts in food availability diversity. Based on assumption that environmental pool microorganisms reduced urban habitats due biodiversity loss, pollution, we hypothesized...
Abstract Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the death of motor neurons, aetiology which essentially unknown. Here, we present an integrative epigenomic study in blood samples from seven clinically characterised sporadic ALS patients to elucidate molecular factors associated with disease. We used clinical exome sequencing (CES) DNA variants, DNA-RNA hybrid immunoprecipitation (DRIP-seq) assess R-loop distribution, and reduced...
ATP-binding cassette (ABC) transporters have key roles in various physiological functions as well providing chemical defense and stress tolerance human tissues. In this study, we examined the expression pattern of all ABC proteins pluripotent embryonic stem cells (hESCs) their differentiated progenies. We paid special attention to cellular localization multidrug transporter proteins.Stem cell differentiation was carried out without induction or sorting, specialized types were separated...
Previously, drug-based synchronization procedures were used for characterizing the cell cycle dependent transcriptional program. However, these methods result in growth imbalance and alteration of machinery. DNA content-based fluorescence activated sorting (FACS) is able to sort different phases without perturbing cycle. MiRNAs are key regulators cycle, however, their expression dynamics during has not been explored. Following an optimized FACS, a complex initiative high throughput platforms...