A5067113035- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- vaccines and immunoinformatics approaches
- Gut microbiota and health
- Evolution and Genetic Dynamics
- Probiotics and Fermented Foods
- Vibrio bacteria research studies
- Biochemical and Structural Characterization
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Mycobacterium research and diagnosis
- Antimicrobial Resistance in Staphylococcus
- Microbial Community Ecology and Physiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Escherichia coli research studies
- Plant Disease Resistance and Genetics
- Microbial bioremediation and biosurfactants
- Plant Pathogens and Resistance
- Fungal Infections and Studies
- Wastewater Treatment and Nitrogen Removal
- Bacterial Genetics and Biotechnology
- Immune Response and Inflammation
- Microbial Metabolism and Applications
University of Oxford
2019-2023
University of Basel
2022-2023
University of Szeged
2019-2023
Mansfield University
2022
Hungarian Research Network
2021
HUN-REN Szegedi Biológiai Kutatóközpont
2018-2021
Hungarian Academy of Sciences
2018-2019
Institute of Biochemistry
2018-2019
Institute of Genomics and Integrative Biology
2012-2017
Council of Scientific and Industrial Research
2012-2017
Abstract Antimicrobial peptides (AMPs) are promising antimicrobials, however, the potential of bacterial resistance is a major concern. Here we systematically study evolution to 14 chemically diverse AMPs and 12 antibiotics in Escherichia coli . Our work indicates that against certain AMPs, such as tachyplesin II cecropin P1, limited. Resistance level provided by point mutations gene amplification very low antibiotic-resistant bacteria display no cross-resistance these AMPs. Moreover,...
Abstract It is well established that antibiotic treatment selects for resistance, but the dynamics of this process during infections are poorly understood. Here we map responses Pseudomonas aeruginosa to in high definition a lung infection single ICU patient. Host immunity and therapy with meropenem suppressed P. , second wave emerged due growth oprD wbpM resistant mutants evolved situ. Selection then led loss resistance by decreasing prevalence low fitness mutants, increasing frequency...
Antimicrobial peptides (AMPs) offer a promising solution to the antibiotic resistance crisis. However, an unresolved serious concern is that evolution of therapeutic AMPs may generate cross-resistance host AMPs, compromising cornerstone innate immune response. We systematically tested this hypothesis using globally disseminated mobile colistin (MCR) has been selected by use in agriculture and medicine. Here, we show MCR provides selective advantage Escherichia coli presence key from humans...
Abstract Functional metagenomics is a powerful experimental tool to identify antibiotic resistance genes (ARGs) in the environment, but range of suitable host bacterial species limited. This limitation affects both scope identified ARGs and interpretation their clinical relevance. Here we present functional pipeline called Reprogrammed Bacteriophage Particle Assisted Multi-species Metagenomics (DEEPMINE). approach combines improves use T7 bacteriophage with exchanged tail fibres targeted...
Abstract Antibiotic resistance tends to carry fitness costs, making it difficult understand how can be maintained in the absence of continual antibiotic exposure. Here we investigate this problem context mcr-1, a globally disseminated gene that confers colistin, an agricultural is used as last resort for treatment multi-drug resistant infections. show regulatory evolution has fine-tuned expression allowing E. coli reduce cost mcr-1 while simultaneously increasing colistin resistance....
Carbaryl (1-naphthyl N-methylcarbamate) is a most widely used carbamate pesticide in the agriculture field. Soil isolate, Pseudomonas sp. strain C5pp mineralizes carbaryl via 1-naphthol, salicylate and gentisate, however genetic organization evolutionary events of acquisition assembly pathway have not yet been studied. The draft genome analysis reveals that catabolic genes are organized into three putative operons, 'upper', 'middle' 'lower'. sequence functional led to identification new...
Antimicrobial peptides (AMPs) are key effectors of the innate immune system and promising therapeutic agents. Yet, knowledge on how to design AMPs with minimal cross-resistance human host-defense remains limited. Here, we systematically assess resistance determinants Escherichia coli against 15 different using chemical-genetics compare spectra laboratory-evolved AMP-resistant strains. Although generalizations about AMP common in literature, find that physicochemical properties cellular...
Staphylococcus capitis TE8 was isolated from skin surface of a healthy human foot, and exhibited strong antibacterial activity against Gram-positive bacteria, including aureus. Whole genome sequence S. obtained by shotgun paired-end pyrosequencing with coverage 109-fold. The draft contains 2,516,639 bp in 8 scaffolds 209 total contigs. 2319 protein coding sequences, 58 tRNA 3 rRNA. Genome analysis revealed 4 distinct gene loci the ability to encode antimicrobial peptides: (i) an epidermicin...
Bacterial pathogens show high levels of chromosomal genetic diversity, but the influence this diversity on evolution antibiotic resistance by plasmid acquisition remains unclear. Here, we address problem in context colistin, a 'last line defence' antibiotic. Using experimental evolution, that carrying MCR-1 colistin gene dramatically increases ability Escherichia coli to evolve high-level acquiring mutations lpxC, an essential involved lipopolysaccharide biosynthesis. Crucially, lpxC...
Trichophyton rubrum is one of the major causative agents dermatophytosis in humans worldwide. We report draft genome sequence T. var. raubitschekii from Delhi, India, isolated a patient presenting symptoms onychomycosis. The total estimated size clinical isolate 25.2 MB containing 8265 predicted protein-coding sequences, 91 tRNA and 15 rRNA genes. Sequence analysis secreted subtilases, virulence factors dermatophytes, clusters them into three subfamilies with distinct features. step...
An alkaliphilic bacterium, Nitritalea halalkaliphila LW7, which belongs to the family Cyclobacteriacae in phylum Bacteroidetes, was isolated from Lonar Lake Maharastra, India. Here we announce draft genome sequence of type strain contains 3,633,701 bp with a G+C content 48.58%.
The negative membrane potential of bacterial cells influences crucial cellular processes. Inspired by the molecular scaffold antimicrobial peptide PGLa, we have developed foldamers with a computer-guided design strategy. novel PGLa analogues induce sustained hyperpolarization. When co-administered as an adjuvant, resulting compounds - PGLb1 and PGLb2 substantially reduced level antibiotic resistance multi-drug resistant Escherichia coli, Klebsiella pneumoniae Shigella flexneri clinical...
Staphylococcus massiliensis strain S46 was isolated from the surface of healthy human skin. Here, we report draft genome sequence S. (2,447,110 bp, with a G+C content 36.3%).
ABSTRACT The genus Nitratireductor represents nitrate-reducing bacteria from the family Phyllobacteriaceae . Here we report draft genome sequence of aquibiodomus strain RA22, which contains 4,592,790 bp, with a G+C content 61.30%, and has 4,241 protein coding genes.
We report the draft genome sequence of carbaryl-degrading Pseudomonas sp. strain C5pp. Genes encoding salicylate and gentisate metabolism, large amounts oxygenase, nitrogen heavy metal tolerance were identified. The will provide further insight into biochemical evolutionary aspects carbaryl degradation.
A wide-host-range bacteriophage (phage) PIS136 was isolated from PA136, a strain of Saccharomonospora belonging to the group actinomycetes. Here, we present genome sequence phage, which is 94,870 bp long and contains 132 putative coding sequences one tRNA gene. An IS element-like region with two genes for transposases identified in genome. The presence suggests that still under active evolution.
Abstract The human gut microbiota has adapted to the presence of antimicrobial peptides (AMPs) that are ancient components immune defence. Despite important medical relevance, it remained unclear whether AMP resistance genes in microbiome available for genetic exchange between bacterial species. Here we show AMP- and antibiotic-resistance differ their mobilization patterns functional compatibilities with new hosts. First, whereas widespread microbiome, rate horizontal transfer is lower than...
We describe the genome sequencing and analysis of a clinical isolate Mycobacterium tuberculosis East African Indian (EAI) strain OSDD271 from India.
Abstract Antimicrobial peptides (AMPs) are key effectors of the innate immune system and promising therapeutic agents. Yet, knowledge on how to design AMPs with minimal cross-resistance human host-defense remains limited. Here, a chemical-genetic approach, we systematically assessed resistance determinants Escherichia coli against 15 different AMPs. Although generalizations about AMP common in literature, found that physicochemical properties cellular targets vary considerably their...
We report the 5.0-Mb genome sequence of Indibacter alkaliphilus strain LW1(T), isolated from a haloalkaline crater lake in Buldana district, Maharashtra, India.
Abstract Antimicrobial peptides (AMPs) offer a promising solution to the antibiotic resistance crisis. However, an unresolved serious concern is that evolution of therapeutic AMPs may generate cross-resistance host AMPs, compromising cornerstone innate immune response. We systematically tested this hypothesis using globally disseminated mobile colistin (MCR) has been selected by use in agriculture and medicine. Here we show MCR provides selective advantage E. coli presence key from humans...