A5062406614- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Evolution and Genetic Dynamics
- DNA and Nucleic Acid Chemistry
- Immune Cell Function and Interaction
- Genomics and Phylogenetic Studies
- Advanced biosensing and bioanalysis techniques
- Vibrio bacteria research studies
- Animal Genetics and Reproduction
- SARS-CoV-2 and COVID-19 Research
- Innovation and Socioeconomic Development
- DNA Repair Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Immunotherapy and Immune Responses
- Gut microbiota and health
- Genetics, Aging, and Longevity in Model Organisms
- Transgenic Plants and Applications
- IL-33, ST2, and ILC Pathways
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
- Insect symbiosis and bacterial influences
- vaccines and immunoinformatics approaches
Tel Aviv University
2015-2024
Arthur M. Sackler Gallery
2013-2014
Harvard University
2006-2009
Ben-Gurion University of the Negev
2003-2006
The clustered regularly interspaced short palindromic repeats and their associated proteins (CRISPR/Cas) constitute a recently identified prokaryotic defense mechanism against invading nucleic acids. Activity of the CRISPR/Cas system comprises three steps: (i) insertion alien DNA sequences into CRISPR array to prevent future attacks, in process called 'adaptation', (ii) expression relevant proteins, as well processing array, followed by (iii) RNA-mediated interference with acid. Here we...
Significance Antibiotic resistance of pathogens is a growing concern to human health, reviving interest in phage therapy. This therapy uses phages (natural bacterial enemies) kill pathogens. However, it encounters many obstacles such as delivery barriers into the tissues and phages. Here, we use for delivering programmable DNA nuclease, clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas), reverse antibiotic eliminate transfer between strains. approach...
Immunotherapies targeting T lymphocytes are revolutionizing cancer therapy but only benefit a subset of patients, especially in colorectal cancer. Thus, additional insight into the tumor microenvironment (TME) is required. Eosinophils bone marrow-derived cells that have been largely studied context allergic diseases and parasite infections. Although tumor-associated eosinophilia has described various solid tumors including cancer, knowledge still missing regarding eosinophil activities even...
Use of bacteriophages as a therapy for bacterial infection has been attempted over the last century. Such an endeavor requires elucidation basic aspects host-virus interactions and resistance mechanisms host. Two recently developed collections now enable genomewide search genetic between Escherichia coli bacteriophages. We have screened >85% E. genes their ability to inhibit growth T7 phage >90% host be used by virus. In addition identifying all known interactions, several other identified....
Pathogen resistance to antibiotics is a rapidly growing problem, leading an urgent need for novel antimicrobial agents. Unfortunately, development of new faces numerous obstacles, and method that resensitizes pathogens approved therefore holds key advantages. We present proof principle system restores antibiotic efficiency by reversing pathogen resistance. This uses temperate phages introduce, lysogenization, the genes rpsL gyrA conferring sensitivity in dominant fashion two antibiotics,...
We show that phage lysogenization, lysogens, and prophage induction are all targeted by CRISPR. The results demonstrate genomic DNA is not immune to the CRISPR system, system does require noncytoplasmic elements, protects from phages entering exiting lysogenic cycle.
The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) system has recently been used to engineer genomes of various organisms, but surprisingly, not those bacteriophages (phages). Here we present a method genetically the Escherichia coli phage T7 using type I-E CRISPR-Cas system. genome is edited by homologous recombination with DNA sequence flanked sequences desired location. Non-edited are targeted system, thus enabling isolation recombinant phages....
Highlights•Hybrid phage particles extend DNA transduction into new bacteria•Directed evolution optimizes particles' bacteria•Optimized hybrid specifically and efficiently transduce desired DNASummaryA major limitation in using bacteriophage-based applications is their narrow host range. Approaches for extending the range have focused primarily on lytic phages hosts supporting propagation rather than approaches ability of phage-restrictive hosts. To T7 transduction, we designed displaying...
BREX (for BacteRiophage EXclusion) is a superfamily of common bacterial and archaeal defence systems active against diverse bacteriophages. While the mechanism currently unknown, self versus non-self differentiation requires methylation specific asymmetric sites in host DNA by BrxX (PglX) methyltransferase. Here, we report that T7 bacteriophage Ocr, mimic protein protects phage from defensive action type I restriction-modification systems, also BREX. In contrast to wild-type phage, which...
Lysis of virus-infected and tumor cells by NK is mediated via natural cytotoxicity receptors (NCRs). We have recently shown that the NKp44 NKp46 NCRs, but not NKp30, recognize viral hemagglutinins. In this study we explored nature cellular ligands recognized NKp30 NCRs. demonstrate target cell surface heparan sulfate proteoglycans (HSPGs) are 6-O-sulfation N-acetylation state glucose building unit affect recognition lysis cells. Tumor expressing heparanase, CHO lacking membranal...
Prokaryotic DNA arrays arranged as clustered regularly interspaced short palindromic repeats (CRISPR), along with their associated proteins, provide prokaryotes adaptive immunity by RNA-mediated targeting of alien or RNA matching the sequences between repeats. Here, we present a thorough screening system for identification bacterial proteins participating in conferred Escherichia coli CRISPR system. We describe one such protein, high-temperature protein G (HtpG), homolog eukaryotic chaperone...
Abstract Bacteriophage T7 is an intracellular parasite that recognizes its host via tail and fiber proteins, known as receptor-binding proteins (RBPs). The RBPs attach to specific lipopolysaccharide (LPS) features on the host. Various studies have shown expansion of phage’s range mutations in genes encoding RBPs, whereas only a few contraction range. Furthermore, most experimental systems not monitored alteration presence several hosts simultaneously. Here we show phage grown five...
Abstract Functional metagenomics is a powerful experimental tool to identify antibiotic resistance genes (ARGs) in the environment, but range of suitable host bacterial species limited. This limitation affects both scope identified ARGs and interpretation their clinical relevance. Here we present functional pipeline called Reprogrammed Bacteriophage Particle Assisted Multi-species Metagenomics (DEEPMINE). approach combines improves use T7 bacteriophage with exchanged tail fibres targeted...
Abstract CRISPR-Cas systems can be utilized as programmable-spectrum antimicrobials to combat bacterial infections. However, how CRISPR nucleases perform across target sites and strains remains poorly explored. Here, we address this knowledge gap by systematically interrogating the use of using multidrug-resistant hypervirulent Klebsiella pneumoniae models. Comparing different Cas nucleases, DNA-targeting outperformed RNA-targeting based on tested targets. Focusing AsCas12a that exhibited...
Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated proteins constitute a recently identified prokaryotic defense system against invading nucleic acids. DNA segments, termed protospacers, are integrated into the CRISPR array in process called adaptation. Here, we establish PCR-based assay that enables evaluating adaptation efficiency of specific spacers type I-E Escherichia coli array. Using this assay, provide direct evidence protospacer adjacent motif...
Significance We have identified a phage-encoded inhibitor of the major cytoskeletal protein in bacterial division. The inhibition is shown to confer T7 bacteriophage with significant growth advantage dividing hosts. Our studies thus indicate strategy bacteriophages maximize their progeny number by inhibiting escape one daughter cells an infected bacterium.
Abstract Despite ongoing efforts to characterize the host response toward SARS-CoV-2, a major gap in our knowledge still exists regarding magnitude and duration of humoral response. Analysis antibody mild versus moderate/severe patients, using new developed quantitative electrochemiluminescent assay for detecting IgM/IgA/IgG antibodies SARS-CoV-2 antigens, revealed rapid onset IgG/IgA antibodies, specifically patients. IgM against viral receptor binding domain, but not nucleocapsid protein,...