A5065556024- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Fecal contamination and water quality
- Bacteriophages and microbial interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Water Treatment and Disinfection
- Antibiotic Resistance in Bacteria
- Mosquito-borne diseases and control
- Salmonella and Campylobacter epidemiology
- Wastewater Treatment and Reuse
- Viral Infections and Vectors
- Child Nutrition and Water Access
- Zoonotic diseases and public health
- COVID-19 diagnosis using AI
- Nanoparticles: synthesis and applications
- Graphene and Nanomaterials Applications
- Molecular Biology Techniques and Applications
- Water Systems and Optimization
- Microplastics and Plastic Pollution
- Hepatitis Viruses Studies and Epidemiology
- Marine Biology and Environmental Chemistry
- Antimicrobial agents and applications
- Vibrio bacteria research studies
University of Lisbon
2017-2025
Instituto Superior Técnico
2013-2025
Institute for Sustainability
2023-2025
Instituto Politécnico de Lisboa
2012-2024
Instituto Superior de Tecnologias Avançadas
2022
Universidad de Ciencias y Humanidades
2021
University of Brighton
2021
Polytechnic Institute of Porto
2016
Antibiotic resistant bacteria (ARB) are a major health risk caused particularly by anthropogenic activities. Acquisition of antibiotic resistances is known to have happened before the discovery antibiotics and can occur through different routes. Bacteriophages thought an important contribution dissemination resistance genes (ARGs) in environment. In this study, seven ARGs (blaTEM, blaSHV, blaCTX-M, blaCMY, mecA, vanA, mcr-1) were investigated, bacteriophage fraction, raw urban hospital...
The most effective strategies to control biofouling release toxic and persistent agents into the aquatic environment causing environmental concerns leading implementation of more strict international legislation. This work presents recent progress on an innovative eco-friendly antifouling approach based isocyanate-functionalized Econea biocide allowing its chemical fixation in polymeric frameworks, namely, a foul-release marine coating. potential generated nonbiocide coatings for both...
Arboviral diseases pose major economic and social threats in less economically developed countries (LEDCs), where monitoring is challenging, especially rapidly growing cities with informal settlements. In this study, we aimed to explore environmental surveillance (ES) a non-sewered setting as complement syndromic Maputo, Mozambique. Water samples were collected from nine points along the Infulene River (n = 66) Mozambique February September 2023. The presence of arboviruses (Dengue (DENV),...
Cardiovascular disease is the main cause of death and incapacity in Portugal. With population ageing greater survival to initial events, burden incapacity-adjusted life years tends increase, representing a growing challenge for health systems. Patients with cardiovascular travel quickly through healthcare systems, brief interactions, by variety professionals, so critical rethinking care approaches. Integrated has shown be effective patients chronic conditions, associated reduced...
Sensitive detection of water- and foodborne enteric viruses is extremely relevant, especially due to the low concentrations in which they are found. Accurate sensitive Norovirus, primary responsible for outbreaks, particular importance. Quantification Norovirus commonly performed by quantitative RT-PCR (RT-qPCR). In recent years a new platform was developed, digital PCR, that quantifies without need standard curve thus decreasing errors associated with its utilization. The developed...
The detection of fecal viral pathogens in water is hampered by their great variety and complex analysis. As traditional bacterial indicators are poor indicators, there a need for alternative methods, such as the use somatic coliphages, which have been included safety regulations recent years. Some researchers also recommended reference noroviruses or other enteric viruses to improve prediction pollution human origin. In this work, phages previously tested microbial source tracking studies...
As the COVID-19 pandemic reached its peak, many countries implemented genomic surveillance systems to track evolution and transmission of SARS-CoV-2. Transition from endemic phase prioritized alternative testing strategies maintain effective epidemic at population level, with less intensive sequencing efforts. One such promising approach was Wastewater-Based Surveillance (WBS), which offers non-invasive, cost-effective means for analysing virus trends sewershed level. From 2020 onwards,...
In response to the rapid emergence and dissemination of antimicrobial resistant bacteria (ARB) genes (ARGs), integrated surveillance systems are needed address resistance (AMR) within One Health Era. Wastewater analyses enable biomarker monitoring at sewershed level, offering timely insights into pathogen circulation ARB/ARGs trends originating from different compartments. During two consecutive epidemic waves COVID-19 pandemic in Portugal, taxonomic functional composition raw urban...
Climate change is altering the habits of population. Extensive drought periods and overuse potable water led to significant shortages in many different places. Therefore, new sources are necessary for usage applications where microbiological chemical quality demands less stringent, as agriculture. In this study, we planted, germinated, grew vegetables/fruits (cherry tomato, lettuce, carrot) using three types potential waters irrigation: secondary-treated wastewater, chlorine-treated green...
To assess the potential of a viability dye and an enzymatic reverse transcription quantitative PCR (RT-qPCR) pretreatment to discriminate between infectious noninfectious enteric viruses.Enterovirus (EntV), norovirus (NoV) GII.4 hepatitis A virus (HAV) were inactivated at 95°C for 10 min, four methods used compare efficiency inactivation: (i) cell culture plaque assay HAV EntV, (ii) RT-qPCR alone, (iii) preceded by RNase treatment, (iv) with (reagent D (RD)) followed RT-qPCR. In addition,...
Water microbial contamination is one of the major threats to human health. The study focus on Infulene River Basin, a urban catchment with mainly informal settlements, limited water supply and sanitation. In there are two wastewater treatment plants, hospital beer factory located banks main stream; from this stream used for agriculture domestic uses by some dwellers. These factors present significant health risk water-borne diseases. At moment knowledge about level different sources at...
No microbial source tracking tool satisfies all the characteristics of an ideal indicator human fecal pollution. For this reason, potential Enterococcus faecalis phages (enterophages) as markers type contamination was tested by using eight strains possible hosts. The prevalence enterophages in animal feces and domestic sewage were determined, inactivation rates raw at 4 °C surface tap waters 22 °C. Enterophages exclusively detected (up to 66.0 plaque forming units (PFU)/100 mL), suggesting a...
Bio-contamination of water through biofouling, which involves the natural colonization submerged surfaces by waterborne organisms, is a global socio-economic concern, allied to premature materials bio-corrosion and high human health risks. Most effective strategies release toxic persistent disinfectant compounds into aquatic medium, causing environmental problems leading more stringent legislation regarding their use. To minimize these side effects, newly non-biocide-release coating strategy...