A5022419058- Bacteriophages and microbial interactions
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Microbial infections and disease research
- Monoclonal and Polyclonal Antibodies Research
- Vibrio bacteria research studies
- Clinical Nutrition and Gastroenterology
- Bacterial Genetics and Biotechnology
- Respiratory viral infections research
- Antibiotic Resistance in Bacteria
- RNA modifications and cancer
- Vitamin D Research Studies
- Microbial Community Ecology and Physiology
- Glycosylation and Glycoproteins Research
- Microscopic Colitis
- Inflammatory Bowel Disease
- Helicobacter pylori-related gastroenterology studies
- Renal Transplantation Outcomes and Treatments
- Nutritional Studies and Diet
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolism and Applications
- Environmental DNA in Biodiversity Studies
- Botanical Research and Chemistry
Technical University of Munich
2020-2025
Helmholtz Zentrum München
2020-2025
Institute of Virology of the Slovak Academy of Sciences
2025
McGill University
2016-2022
Stockholm University
2014-2019
Phage therapy, treating bacterial infections with bacteriophages, could be a future alternative to antibiotic treatment of infections. There are, however, several problems solved, mainly associated the biology phages, interaction between phages and their hosts, but also vast variation pathogenic bacteria which implies that large numbers different are going needed. All these must under present regulation medical products undergo extensive clinical testing before they can applied. It will...
Ulcerative colitis is an idiopathic gastrointestinal disease described by chronic inflammation of the digestive system. Cytokines may be responsible for immunopathogenesis, mucosal and tissue damage, even treatment response. In addition to its role in calcium phosphorus homeostasis bone health, vitamin D immunomodulatory anti-inflammatory agent. Understanding cytokines lead improving pathogenesis this disease, therefore we aimed investigate relative gene expression pro- biopsy samples taken...
Stunting, a severe and multigenerational growth impairment, globally affects 22% of children under the age 5 years. Stunted have altered gut bacterial communities with higher proportions Proteobacteria, phylum several known human pathogens. Despite links between an microbiota stunting, role bacteriophages, highly abundant viruses, is unknown. Here, we describe bacteriophage Bangladeshi stunted younger than 38 months. We show that these harbor distinct bacteriophages relative to their...
Abstract Background Inflammatory bowel diseases (IBDs) including Crohn’s disease (CD) and ulcerative colitis (UC) are characterized by chronic debilitating gut inflammation. Altered bacterial communities of the intestine strongly associated with IBD initiation progression. The virome, which is primarily composed viruses (bacteriophages, phages), thought to be an important factor regulating shaping microbial in gut. While alterations virome have been observed patients, contribution these...
Abstract Transition from traditional high-fiber to Western diets in urbanizing communities of Sub-Saharan Africa is associated with increased risk non-communicable diseases (NCD), exemplified by colorectal cancer (CRC) risk. To investigate how urbanization gives rise microbial patterns that may be amenable dietary intervention, we analyzed diet intake, fecal 16 S bacteriome, virome, and metabolome a cross-sectional study healthy rural urban Xhosa people (South Africa). Urban individuals had...
Antibiotic resistance causes around 700,000 deaths a year worldwide. Without immediate action, we are fast approaching post-antibiotic era in which common infections can result death. Pseudomonas aeruginosa is the leading cause of nosocomial infection and also one three bacterial pathogens WHO list priority bacteria for developing new antibiotics against. A viable alternative to use phages, viruses. Yet, isolation phages that efficiently kill their target has proven difficult. Using...
Abstract Background P. aeruginosa is the primary source of hospital-acquired infections. Unfortunately, antibiotic resistance growing to precariously high levels, making infections by this pathogen life-threatening and hard cure. One possible alternative antibiotics use phages. However, isolation phages suitable for phage therapy— be lytic, efficient, have a broad host range —against some target bacteria has proven difficult. To identify best places look these against we screened hospital...
Bacteriophages play central roles in the maintenance and function of most ecosystems by regulating bacterial communities. Yet, our understanding their diversity remains limited due to lack robust bioinformatics standards. Here we present ViroProfiler, an in-silico workflow for analyzing shotgun viral metagenomic data. ViroProfiler can be executed on a local Linux computer or cloud computing environments. It uses containerization technique ensure computational reproducibility facilitate...
A recently isolated phage, vB_EcoP_SU10 (SU10), with the unusual elongated C3 morphotype, can infect a wide range of Escherichia coli strains. We have sequenced genome this phage and characterized it further by mass spectrometry based proteomics, transmission electron microscopy (TEM), scanning (SEM), ultra-thin section microscopy. The size is 77,327 base pairs its genes, architecture, show high similarity to phiEco32 genes genome. TEM images reveal that SU10 quite long tail for being...
strains exhibit varying associations with atopic dermatitis (AD), but the genetic determinants underpinning pathogenicity are yet to be fully characterized. To reveal differences between
Due to a global increase in the range and number of infections caused by multi-resistant bacteria, phage therapy is currently experiencing resurgence interest. However, there are well-known concerns over use phages treat bacterial infections. In order address safety poorly understood pharmacokinetics their associated cocktails, immunological characterization required. current investigation, immunogenicity four distinct (taken from main families that comprise Caudovirales order) interaction...
ABSTRACT The rapid increase in antibiotic resistance has led to a renewed interest phage therapy, which created need for the establishment of collections order preserve diverse phages and reduce delivery time patients. However, there are currently no standard methods long-term preservation phages. We assessed stability four different under distinct storage conditions, including temperatures, solutions, concentrations, with or without cryoprotectant. found that type buffer significant impact...
Colorectal cancer (CRC) is one of the most commonly diagnosed cancers worldwide. While a close correlation between chronic Helicobacter pylori infection and CRC has been reported, role virome overlooked. Here, we infected Apc-mutant mouse models C57BL/6 mice with H. conducted comprehensive metagenomics analysis pylori-induced changes in lower gastrointestinal tract bacterial viral communities. We observed an expansion temperate phages Apc+/1638N at early stage carcinogenesis. Some were...