A5089522092- Hepatitis C virus research
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- HIV/AIDS drug development and treatment
- Hepatitis Viruses Studies and Epidemiology
- Hepatitis B Virus Studies
- Animal Virus Infections Studies
- Liver Disease Diagnosis and Treatment
- HIV Research and Treatment
- Herpesvirus Infections and Treatments
- Paraquat toxicity studies and treatments
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Trace Elements in Health
- Toxin Mechanisms and Immunotoxins
- HIV/AIDS Research and Interventions
- Cytomegalovirus and herpesvirus research
- Plant Virus Research Studies
- Pharmacological Effects of Natural Compounds
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Pesticide and Herbicide Environmental Studies
- Viral-associated cancers and disorders
UK Health Security Agency
2024
Public Health England
2015-2021
Universidade de Santiago de Compostela
2010-2015
Approximately 10% of German-bred Pekin ducks were found to be chronically infected with duck hepatitis B virus (DHBV). The genomes three German DHBV isolates analyzed closely related but showed substantial restriction site polymorphism compared U.S. isolates. We tested the infectivity sequence variants cloned DNA by injecting them into liver virus-free ducklings. Most these animals injected double-stranded closed-circular or plasmid-integrated dimer developed viremia, demonstrating all...
Abstract Sustained viral response (SVR) rates for direct‐acting antiviral (DAA) therapy hepatitis C virus (HCV) infection routinely exceed 95%. However, a small number of patients require retreatment. Sofosbuvir, velpatasvir and voxilaprevir (SOF/VEL/VOX) is potent DAA combination primarily used the retreatment who failed by therapies. Here we evaluate outcomes effects resistance‐associated substitutions (RAS) in real‐world cohort, including large genotype (GT)3 infected patients. 144 from...
RNA viruses cause significant human pathology and are responsible for the majority of emerging zoonoses. Mainstream diagnostic assays challenged by their intrinsic diversity, leading to false negatives incomplete characterisation. New sequencing techniques expanding our ability agnostically interrogate nucleic acids within diverse sample types, but in clinical setting limited overwhelming host material ultra-low target frequency. Through selective depletion compensatory protocol adjustments...
Abstract Background Hepatitis C virus (HCV) has high genetic diversity and is classified into 8 genotypes >90 subtypes, with some endemic to specific world regions. This could compromise direct-acting antiviral efficacy global HCV elimination. Methods We characterized subtypes “rare” in the United Kingdom (non-1a/1b/2b/3a/4d) by means of whole-genome sequencing via a national surveillance program. Genetic analyses determine genotype samples unresolved were undertaken comparison...
Using deep sequencing technologies such as Illumina's platform, it is possible to obtain reads from the viral RNA population revealing genome diversity within a single host. A range of software tools and pipelines can transform raw into Sequence Alignment Mapping (SAM) files. We propose that interpretation should process these SAM files, directly translating individual amino acids in order extract statistics interest proportion different acid residues at specific sites. This preserves...
Choice of direct acting antiviral (DAA) therapy for Hepatitis C Virus (HCV) in the UK and similar settings usually requires knowledge genotype and, some cases, resistance (AVR) profile infecting virus. To determine these, most laboratories currently use Sanger technology, but next generation sequencing (NGS) offers potential advantages throughput accuracy. However, NGS poses unique technical challenges, which require idiosyncratic development validation approaches. This applies particularly...
HIV treatment guidelines have traditionally recommended that all HIV-positive individuals are tested for evidence of drug resistance prior to starting ART. Testing reverse transcriptase inhibitors and PIs is well established in routine care. However, testing integrase strand transfer inhibitor (InSTI) less consistent.To inform by determining the prevalence InSTI a national cohort recently infected individuals.Recent (within 4 months) HIV-1 infections were identified using Recent Infection...
Hepatitis A virus (HAV) represents a significant public health problem due to its high persistence in the environment and transmission through contaminated water food. Bivalve shellfish are filter feeders that can bioaccumulate human pathogens found waters, their consumption being potential cause of hepatitis outbreaks. In this work, cultured wild bivalve from Ría de Vigo (Galicia, NW Spain) were analysed for presence genotyping HAV. total 160 samples collected between March 2004 December...
The long and expanding list of viral pathogens associated with causing encephalitis confounds current diagnostic procedures, in up to 50% cases, the etiology remains undetermined. Sequence-agnostic metagenomic next-generation sequencing (mNGS) obviates need specify targets advance thus has great potential diagnostics. However, low relative abundance nucleic acids clinical specimens poses a significant challenge. Our protocol employs two novel techniques selectively remove human material at...
Abstract A total of 2,643 samples from patients with gastroenteritis in Galicia (NW Spain) were tested for the presence Norovirus (NoV). NoV genogroup GI was detected 416 (15.7%) samples, while GII 278 (10.5%) samples. Mixed infections and observed 53 (2%) Total prevalence analyzed 28.3%. Besides diagnosis assay, all specimens also submitted to routine clinical bacteriology tests. Cryptosporidium spp. as well adenovirus (AdV) rotavirus (RV) determined on some after specific request by...
SUMMARY The high incidence of norovirus (NoV) infections seems to be related the emergence new variants that evolved by genetic drift capsid gene. In this work, represents a first effort describe molecular epidemiology NoV in northwest Spain, total eight different genotypes (GII.1, GII.3, GII.4, GII.6, GII.7, GII.12, GII.13, GII.14) were detected. major observed GII.4 (45·42%) and GII.14 (34·9%), being detected all age groups. addition, although most sequences belonged 2006b (7·2%) 2010...
Herpes simplex virus type 1 (HSV-1) and 2 (HSV-2) infections may be life-threatening in immunocompromised patients. Antiviral treatment is available but resistance can develop. The gold standard for testing cell culture-based phenotyping which slow (3–4 weeks) has a high (50 %) failure rate. Faster (<10 working days) genotypic of viral thymidine kinase (TK) DNA polymerase (DNApol) used with >95 % success However, reference database interpreting drug susceptibility from genetic data...
Abstract Sustained viral response (SVR) rates to first-line Direct Acting Antiviral (DAA) therapy for hepatitis C virus (HCV) infection routinely exceed 95%. However, a small number of patients require retreatment. Sofosbuvir, velpatasvir and voxilaprevir (SOF/VEL/VOX) is potent DAA combination primarily used the retreatment failed by first line therapies. Here we evaluate outcomes effects resistance associated substitutions (RAS) in real-world cohort, including largest genotype (GT)3...