A5038520189- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Microbial infections and disease research
- CRISPR and Genetic Engineering
- Vibrio bacteria research studies
- Bacterial Identification and Susceptibility Testing
- Evolution and Genetic Dynamics
- Bacterial Genetics and Biotechnology
- Gut microbiota and health
- Mollusks and Parasites Studies
- Advanced biosensing and bioanalysis techniques
- Wastewater Treatment and Nitrogen Removal
- Biosensors and Analytical Detection
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- Microbial Community Ecology and Physiology
- Nematode management and characterization studies
- Identification and Quantification in Food
- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Aquaculture disease management and microbiota
- Legume Nitrogen Fixing Symbiosis
- Respiratory viral infections research
University of Oxford
2023-2024
Universidad Nacional Autónoma de México
2017-2023
King Saud University
2023
Wellcome Sanger Institute
2022
Universidad Autónoma del Estado de Morelos
2019
The genus Serratia has been studied for over a century and includes clinically-important diverse environmental members. Despite this, there is paucity of genomic information across the robust whole genome-based phylogenetic framework lacking. Here, we have assembled analysed representative set 664 genomes from genus, including 215 historic isolates originally used in defining genus. Phylogenomic analysis reveals clearly-defined population structure which displays deep divisions aligns with...
Background Bacteriophage therapy is becoming part of mainstream Western medicine since antibiotics clinical use tend to fail. It involves applying lytic bacteriophages that self-replicate and induce cell lysis, thus killing their hosts. Nevertheless, bacterial promotes the selection resistant clones which sometimes may exhibit a decrease in virulence or antibiotic resistance. Methods In this work, we studied Pseudomonas aeruginosa phage φDCL-PA6 its variant φDCL-PA6α. Additionally,...
Phages and plasmids are discrete mobile genetic elements (MGEs) with critical roles in gene dissemination across bacteria but limited scope for exchanging DNA between them. By investigating recent gene-sharing events, Pfeifer Rocha describe how the hybrid phage-plasmids (P-Ps) promote flow MGE types evolve into new ones.
Phages are generally described as species specific or even strain specific, implying an inherent limitation for some to be maintained and spread in diverse bacterial communities. Moreover, phage isolation host range determination rarely consider the ecological context, likely biasing our notion on specificity. Here we isolated characterized a novel group of six promiscuous phages, named Atoyac, existing rivers sewage by using collection over 600 bacteria retrieved from same environments...
Host-parasite interactions are highly susceptible to changes in temperature due mismatches species thermal responses. In nature, parasites often exist communities, and responses expected vary between host-parasite pairs. Temperature change thus has consequences for both dynamics parasite-parasite interactions. Here, we investigate the impact of warming (37°C, 40°C, 42°C) on parasite life-history traits competition using opportunistic bacterial pathogen
Quorum sensing (QS) in Pseudomonas aeruginosa coordinates the expression of virulence factors, some which are used as public goods. Since their production is a cooperative behavior, it susceptible to social cheating non-cooperative QS deficient mutants use resources without investing production. Nevertheless, functional systems abundant; hence, mechanisms regulating amount should exist. Evidence that demonstrates tight relationship between and susceptibility bacteria against attack lytic...
Abstract Plasmids are now the primary vectors of antimicrobial resistance, but our understanding how human industrialisation antibiotics influenced this is limited by a paucity data predating antibiotic era (PAE). By investigating plasmids from clinically relevant bacteria isolated between 1917 and 1954 comparing them to modern plasmids, we captured over 100 years evolution. We show that while all PAE were devoid resistance genes most never acquired them, small minority evolved drive global...
Microbiomes are vast communities of microbes and viruses that populate all natural ecosystems. Viruses have been considered the most variable component microbiomes, as supported by virome surveys examples high genomic mosaicism. However, recent evidence suggests human gut is remarkably stable compared to other environments. Here we investigate origin, evolution, epidemiology crAssphage, a widespread virus. Through global collaboratory, obtained DNA sequences crAssphage from over one-third...
Conjugative transfer is one of the mechanisms allowing diversification and evolution bacteria. Rhizobium etli CFN42 a bacterial strain whose habitat rhizosphere able to form nodules as result nitrogen-fixing symbiotic relationship it may establish with roots Phaseolus vulgaris. R. contains chromosome six large plasmids (pRet42a - pRet42f). Most genetic information involved in establishment symbiosis localized on plasmid pRet42d, named (pSym). This perform conjugation, using pSym encoded...
Anti-clustered regularly interspaced short palindromic repeats (CRISPRs) are proteins capable of blocking CRISPR-Cas systems and typically their genes located on mobile genetic elements. Since discovery, numerous anti-CRISPR families have been identified. However, little is known about the distribution sequence diversity members within a family, nor how these traits influence anti-CRISPR’s function evolution. Here, we use AcrIF7 to explore dissemination molecular evolution an family. We...
Social cheating is the exploitation of public goods that are costly metabolites, like exoproteases. Exoprotease in Pseudomonas aeruginosa has been studied reference strains. Experimental evolution with strains during continuous growth casein demonstrated nonexoprotease producers lasR mutants selected while they behave as social cheaters. However, noncanonical quorum-sensing systems exist P. strains, which diverse. In this work, exoproteases environmental strain ID4365 was evaluated; a...
Abstract Phages are generally described as species- or even strain-specific viruses, implying an inherent limitation for some to be maintained and spread in diverse bacterial communities. Moreover, phage isolation host range determination rarely consider the ecological context, likely biasing our notion on specificity. Here we identified characterized a novel group of promiscuous phages existing rivers by using bacteria isolated from same samples, then used this biological system investigate...
Abstract The genus Serratia has been studied for over a century and includes clinically-important diverse environmental members. Despite this, there is paucity of genomic information across the robust whole genome-based phylogenetic framework lacking. Here, we have assembled analysed representative set 664 genomes from genus, including 215 historic isolates originally used in defining genus. Phylogenomic analysis reveals clearly-defined population structure which displays deep divisions...
ABSTRACT Anti-CRISPRs are proteins capable of blocking CRISPR-Cas systems and typically encoded in mobile genetic elements. Since their discovery, numerous anti-CRISPR families have been identified. However, little is known about the distribution sequence diversity members within a family, nor how these traits influence anti-CRISPR’s function evolution. Here we use AcrIF7 to explore dissemination molecular evolution an family. We uncovered five sub-clusters prevalent variants group....