A5035323786- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Microbial infections and disease research
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Bacterial Identification and Susceptibility Testing
- Advanced biosensing and bioanalysis techniques
- Antibiotics Pharmacokinetics and Efficacy
- Pneumonia and Respiratory Infections
- Pharmaceutical and Antibiotic Environmental Impacts
- Oral microbiology and periodontitis research
- Antimicrobial Resistance in Staphylococcus
- Enterobacteriaceae and Cronobacter Research
- Escherichia coli research studies
- Biosensors and Analytical Detection
- Distributed and Parallel Computing Systems
- Antimicrobial Peptides and Activities
- Infective Endocarditis Diagnosis and Management
- RNA and protein synthesis mechanisms
- Streptococcal Infections and Treatments
- Infections and bacterial resistance
- Nosocomial Infections in ICU
Instituto de Investigación Biomédica de A Coruña
2016-2025
Complexo Hospitalario Universitario A Coruña
2016-2025
Universidade da Coruña
2016-2025
Biomedical Research Institute
2023-2025
Fundación Seimc-Gesida
2020-2024
Hospital General Universitario Gregorio Marañón
2024
Red Espanola de Investigacion en Patologia Infecciosa
2013-2022
Instituto de Salud Carlos III
2017-2022
Centro de Investigación Biomédica en Red
2022
Servicio Gallego de Salud
2018-2022
Abstract In contrast to the many reports of successful real-world cases personalized bacteriophage therapy (BT), randomized controlled trials non-personalized products have not produced expected results. Here we present outcomes a retrospective observational analysis first 100 consecutive BT difficult-to-treat infections facilitated by Belgian consortium in 35 hospitals, 29 cities and 12 countries during period from 1 January 2008 30 April 2022. We assessed how often positive clinical...
To evaluate the performance of oxacillin, cefazolin, cefoxitin, cefotaxime and imipenem discs; Etest for oxacillin; microdilution; agar screening plates with 2 6 mg/L PBP2' agglutination detection methicillin-resistant Staphylococcus aureus (MRSA).A total 102 clinical S. isolates, including 51 MRSA tested by PCR presence or absence mecA gene (gold standard method), isolated from different patients at times, were with: oxacillin (1 microg), (all 30 microg) microdilution tests (ORSAB medium)...
ABSTRACT We investigated the mechanisms of resistance to carbapenems, aminoglycosides, glycylcyclines, tetracyclines, and quinolones in 90 multiresistant clinical strains Acinetobacter baumannii isolated from two genetically unrelated A. clones: clone PFGE-ROC-1 (53 producing OXA-58 β-lactamase enzyme 18 with OXA-24 β-lactamase) PFGE-HUI-1 (19 susceptible carbapenems). used real-time reverse transcriptase PCR correlate antimicrobial (MICs) expression genes encoding chromosomal β-lactamases...
ABSTRACT Expression of ampC , oprD mexA mexC mexE and mexX was studied in 25 Pseudomonas aeruginosa isolates from cystic fibrosis patients, including 14 the Liverpool epidemic strain. Overexpressed or reduced were associated with β-lactam resistance. A specific combination mexR nalC nalD mutations occurred 11 strain isolates, 7 upregulated .
Acinetobacter baumannii has emerged as a dangerous opportunistic pathogen, with many strains able to form biofilms and thus cause persistent infections. The aim of the present study was use high-throughput sequencing techniques establish complete transcriptome profiles planktonic (free-living) sessile (biofilm) forms A. ATCC 17978 thereby identify differences in their gene expression patterns. Collections mRNA from (both exponential stationary phase cultures) cells were sequenced. Six...
Acinetobacter baumannii is an extracellular opportunistic human pathogen that becoming increasingly problematic in hospitals. In the present study, we demonstrate A. Omp 33- to 36-kDa protein (Omp33-36) a porin acts as channel for passage of water. The found on cell surface and released along with other porins outer membrane vesicles (OMVs). immune connective tissue, this induced apoptosis by activation caspases modulation autophagy, consequent accumulation p62/SQSTM1 (sequestosome 1)...
Desiccation tolerance contributes to the maintenance of bacterial populations in hospital settings and may partly explain its propensity cause outbreaks. Identification relative quantitation proteins involved desiccation was made using label-free iTRAQ labeling. Under desiccating conditions, population Acinetobacter baumannii clinical strain AbH12O-A2 decreased first week, thereafter, a stable 0.5% original maintained. Using labeling, 727 765 proteins, respectively, were detected; 584 them...
BackgroundTreatment of multidrug-resistant Acinetobacter baumannii (MDRAB) infection presents a challenge because the scarcity available options. Even though combination therapy (CT) is frequently used in clinical practice, data are needed to support its use instead monotherapy (MT).
The emergence of multidrug resistant (MDR) pathogenic bacteria is jeopardizing the value antimicrobials, which had previously changed course medical science. In this study, we identified endolysins ElyA1 and ElyA2 (GH108-PG3 family), present in genome bacteriophages Ab1051Φ Ab1052Φ, respectively. muralytic activity these against MDR clinical isolates (Acinetobacter baumannii, Pseudomonas aeruginosa Klebsiella pneumoniae) was tested using turbidity reduction assay. Minimal inhibitory...
Clinical case of a patient with Pseudomonas aeruginosa multidrug-resistant prosthetic vascular graft infection which was treated cocktail phages (PT07, 14/01, and PNM) in combination ceftazidime-avibactam (CZA). After the application phage treatment absence antimicrobial therapy, new P. bloodstream (BSI) septic residual limb metastasis occurred, now involving wild-type strain being susceptible to ß-lactams quinolones. strains were analyzed by microbiology whole genome sequencing techniques....
The objective of this study was to analyse whether there is an association between reduced susceptibility biocides in Acinetobacter baumannii and (i) antimicrobial resistance (co-resistance), (ii) prevalent (epidemic) clones, (iii) changes the fitness or (iv) expression genes related efflux pumps porins. Susceptibility antimicrobials determined 49 clonally unrelated isolates A. baumannii. Biological cost, terms mean generation time, by spectrophotometry. Quantitative real-time RT–PCR used...
Acinetobacter baumannii presents a typical luxI/luxR quorum sensing (QS) system (abaI/abaR) but the acyl-homoserine lactone (AHL) signal profile and factors controlling production of QS signals in this species have not been determined yet. A very complex AHL was identified for A. ATCC17978 as well nosocomialis M2, only when cultivated under static conditions, suggesting that surface or cell-to-cell contact is involved activation genes. The analysis baumanni clinical isolates revealed...
Quorum sensing (QS) in Pseudomonas aeruginosa coordinates the expression of virulence factors, such as exoproteases and siderophores, that are public goods utilized by whole population bacteria, regardless whether they invested or not their production. These can be used QS defective mutants for growth since these do contribute to production, considered social cheaters. Pyocyanin is a phenazine toxic, QS-controlled metabolite produced P. aeruginosa. It redox-active compound promotes...
Klebsiella pneumoniae is an opportunistic Gram-negative pathogen that employs different strategies (resistance and persistence) to counteract antibiotic treatments. This study aimed search for new means of combatting imipenem-resistant persister strains K. by repurposing the anticancer drug mitomycin C as antimicrobial agent combining conventional imipenem with lytic phage vB_KpnM-VAC13. Several clinical isolates were characterized, isolate (harboring OXA-245 β-lactamase) a selected study....
The need for alternatives to antibiotic therapy due the emergence of multidrug resistant bacteria (MDR), such as nosocomial pathogen Acinetobacter baumannii, has led recovery phage therapy. In addition, phages can be combined in cocktails increase host range. this study, evolutionary mechanism adaptation was utilized order develop a adapted A. named Ab105-2phiΔCI404ad, from mutant lytic phage, Ab105-2phiΔCI, previously developed by our group. whole genome sequence Ab105-2phiΔCI404ad...
Abstract Antibiotic failure is one of the most worrisome threats to global health. Among new therapeutic efforts that are being explored, use bacteriophages (viruses kill bacteria), also known as ‘phages’, extensively studied a strategy target bacterial pathogens. However, main drawbacks phage therapy plethora defence mechanisms bacteria defend themselves against phages. This review aims summarize approaches evaluated overcome systems, including innovative applied: circumvention receptor...