ABSTRACT The resistance of Acinetobacter baumannii strains to carbapenems is a worrying problem in hospital settings. main mechanism carbapenem the expression β-lactamases (metalloenzymes or class D enzymes). mechanisms dissemination these genes among A. are not fully understood. In this study we used two carbapenem-resistant clinical (AbH12O-A2 and AbH12O-CU3) expressing plasmid-borne bla OXA-24 gene (plasmids pMMA2 pMMCU3, respectively) demonstrate that releases outer membrane vesicles...

Acinetobacter baumannii is an extracellular opportunistic human pathogen that becoming increasingly problematic in hospitals. In the present study, we demonstrate A. Omp 33- to 36-kDa protein (Omp33-36) a porin acts as channel for passage of water. The found on cell surface and released along with other porins outer membrane vesicles (OMVs). immune connective tissue, this induced apoptosis by activation caspases modulation autophagy, consequent accumulation p62/SQSTM1 (sequestosome 1)...

When cultured in broth to the transmissive phase, Legionella pneumophila infects macrophages by inhibiting phagosome maturation, whereas replicative-phase cells are transported lysosomes. Here we report that ability of L. inhibit phagosome-lysosome fusion correlated with developmentally regulated modifications pathogen's surface, as judged its lipopolysaccharide profile and binding a sialic acid-specific lectin hydrocarbon hexadecane. Likewise, composition membrane vesicles shed was...

This study focused on estimating the prevalence of extended-spectrum β-lactamases (ESBLs), plasmid-mediated AmpC β-lactamases, carbapenemases and MCR-1-producing Escherichia coli in canine faeces from a public park Quito, Ecuador. Phenotypic genotypic characterisation E. isolated 50 faecal samples recovered city Quito was performed. In addition, multiple choice survey conducted among dog owners. Of samples, 20 (40.0%) presented resistant to ceftriaxone. Moreover, 23 isolates were for further...

The neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte (PLR), lymphocyte-to-monocyte (LMR), and mean platelet volume-to-platelet (MPR) are combined hematology tests that predict COVID-19 severity, although with different cut-off values. Because sex significantly impacts immune responses the course of COVID-19, ratios could be biased by sex.This study aims to evaluate sex-dependent differences in contribution NLR, PLR, MLR, MPR severity mortality upon hospital admission using a...

Acinetobacter baumannii is an opportunistic pathogen that has been associated with severe infections and outbreaks in hospitals. At present, very little known about the biology of this bacterium, particularly as regards mechanisms adaptation, persistence virulence. To investigate growth phase-dependent regulation proteins microorganism, we analyzed proteomic pattern A. ATCC 17978 at different stages vitro growth. In study, proteomics analyses were conducted using 2-DE MALDI-TOF/TOF...

Antimicrobial resistance (AMR) is a major health threat for public and animal in the twenty-first century. In Ecuador, antibiotics have been used by poultry industry decades resulting presence of multi-drug resistant (MDR) bacteria meat production chain, with consequent risk health. This study evaluated prevalence ESBL/AmpC mcr genes third-generation cephalosporin-resistant Escherichia coli (3GC-R E. ) isolated from broiler farms (animal component), carcasses (food human enteritis (human...

The inappropriate use of antibiotics leads to antibiotic resistance, which reduces their efficacy. education undergraduates is likely influence practices. Assessing awareness critical in the general effort confront spread resistance. This cross-sectional investigation was carried out using questionnaire “Antibiotic resistance: Multi-country public awareness” developed by World Health Organization. Students from different backgrounds at Central University participated study (n = 733). survey...

Twelve 7-chloroquinoline derivatives were designed and synthesized using the principle of molecular hybridization through coupling 2-[2-(7-chloroquinolin-4-ylthio)-4-methylthiazol-5-yl]acetic acid 1 with various benzoyl hydrazines 2a-l. The synthetic compounds tested as antimalarials. Some them showed an efficient in vitro activity inhibitors β-hematin formation vivo a murine model, resulting 8 9 most active ones IC50 values 0.65 ± 0.09 0.64 0.16 µM, respectively. effects on cell viability,...

Article Common laboratory tests as indicators of COVID-19 severity on admission at high altitude: a single-center retrospective study in Quito (ECUADOR) was published July 1, 2021 the journal Clinical Chemistry and Laboratory Medicine (CCLM) (volume 59, issue 8).

ABSTRACT The gene encoding the DNA gyrase A subunit of Streptococcus pneumoniae was cloned and sequenced. gyrA codes for a protein 822 amino acids homologous to eubacteria. Translation in an Escherichia coli expression system revealed 92-kDa polypeptide. sequence-directed curvature identified promoter region . bend center mapped located between −35 −10 regions promoter. Primer extension analysis showed that transcription initiates 6 bp downstream extended possible implications bent as...

After uptake by susceptible host cells, Legionella pneumophila displays the ability to arrest phagolysosome fusion. To elucidate role of lipopolysaccharide (LPS) in this mechanism, we investigated its influence on Acanthamoeba castellanii, A/J mouse macrophages and human monocytes. For this, legionellae were cultured broth replicative, noninfectious phase or infectious expressing virulence traits. Shed LPS-enriched outer membrane vesicles (OMV) LPS species <300 kDa obtained from L. Corby...

This pilot investigation aimed at studying the feasibility of using a low dose (0.2%) dietary microalgae as means improving intestinal morphometry, body weight, and selected meat quality parameters in broilers. A total 72 one-day-old ROSS 308 male chicks were randomly separated into four groups; three experimental pens which birds fed with biomass from Tysochrysis lutea, Tetraselmis chuii, Porphyridium cruentum over 30 days control group. T. chuii P. had positive effect regard to weight. In...

The COVID-19 pandemic poses challenges in terms of diagnosis, as existing tests often fail to consistently distinguish pneumonia from other acute respiratory infections. Although the integration machine learning (ML) with diagnostic procedures holds promise addressing this issue, a comprehensive understanding current scientific relevance and trends approach has not yet been undertaken. In study, it was conducted bibliometric analysis explore significance developing ML-based algorithms for...

The genes encoding the subunits of DNA topoisomerase IV (parC and parE) gyrase (gyrA gyrB) Streptococcus pneumoniae were cloned overproduced in Escherichia coli by using T7promoter-T7 RNA polymerase system. four separately purified to near homogeneity column chromatography. Protein purification was achieved DEAE-sepharose, heparin-agarose, hydroxylapatite reconstituted when ParC ParE combined at a 3.8-fold excess ParE. showed generate efficient ATP-dependent decatenation activity....

Antimicrobial resistance genes are often associated with integrons, which promote their movement between and within DNA molecules. IntFinder 1.0 I-VIP v1.2 were used for the detection of integrons in assembled sequences raw reads. A dataset comprising 1688 sequenced Salmonella enterica isolates from countries Andean Community was developed. total 749 680 identified by v1.2, respectively; class 2 most abundant followed 1, whereas no 3 detected. These elements mainly animal sources. S....

A series of benzocycloalkanone derivatives have been prepared and evaluated as antimalarial antitrypanosomal agents. The compounds were obtained by direct coupling preformed 4-substituted benzaldehyde indanone or tetralone substitutes through aldol condensation Claisen-Schmidt using sodium hydroxide a catalyst in ethanol at room temperature. Although designed to inhibit the formation β-hematin vitro, only three compounds, 10, 11, 12, showed activities greater than 50% (75.16%, 63.02%,...

Adherence to preventive measures is influenced by people’s knowledge, attitudes and practices towards a disease; therefore, assessing knowledge of COVID-19 critical in the overall effort contain outbreak. This cross-sectional study was conducted among undergraduates (n = 3621) different programs levels education associated with universities north-central Ecuador. The form consisted 32 questions covering demographics, symptoms, detection, treatment, transmission, prevention virus. rate...

Leukemia is associated with exposure to radiation, benzene derivatives, and pesticides. Previous research has documented an increase in work-related leukemia the Latin American Andean region. To date, there are only few studies Ecuador on impact of oil exploitation adjacent indigenous communities. Our study aims show working-age population. For calculation morbidity mortality rates, we used hospital discharge death records from National Institute Statistics Ecuador. These data were collected...

The aim of this work was to obtain and evaluate, as antiprotozoals, new derivatives benzoate imidazo-1,3,4-thiadiazole 18–23 based on the concepts molecular repositioning hybridization. In design these compounds, two important pharmacophoric subunits fexnidazole prototype were used: metronidazole used a molecule, p-aminobenzoic acid incorporated bridge group, 1,3,4-thiadiazole group second pharmacophore, which at position 5 has an aromatic with different substituents incorporated. final six...