A5031414882- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Advanced biosensing and bioanalysis techniques
- Bacterial biofilms and quorum sensing
- Plant Virus Research Studies
- Aquaculture disease management and microbiota
- Glycosylation and Glycoproteins Research
- Microbial Community Ecology and Physiology
- Cystic Fibrosis Research Advances
- Monoclonal and Polyclonal Antibodies Research
- Transgenic Plants and Applications
- RNA and protein synthesis mechanisms
Instituto de Investigación Biomédica de A Coruña
2022-2025
Complexo Hospitalario Universitario A Coruña
2022-2025
Universidade da Coruña
2022-2025
Fundación Seimc-Gesida
2022-2024
Abstract Antibiotic failure is one of the most worrisome threats to global health. Among new therapeutic efforts that are being explored, use bacteriophages (viruses kill bacteria), also known as ‘phages’, extensively studied a strategy target bacterial pathogens. However, main drawbacks phage therapy plethora defence mechanisms bacteria defend themselves against phages. This review aims summarize approaches evaluated overcome systems, including innovative applied: circumvention receptor...
Abstract Phage tail-like bacteriocins (PTLBs) are large proteomic structures similar to the tail phages. These function in bacterial competition by making pores membrane of their competitors. The PTLBs identified Pseudomonas aeruginosa known as R-type and F-type pyocins, which have a narrow spectrum action. Their specificity is determined fiber closely related lipopolysaccharide type target competitor strain. In this study, genome sequences 32 clinical P. isolates were analysed investigate...
Researchers, including microbiologists and infectious disease specialists, require more knowledge about the interactions between phages their bacterial hosts defense mechanisms. In this study, we analyzed molecular mechanisms of viral in infecting clinical isolates K. pneumoniae .
Multidrug-resistant bacteria and the shortage of new antibiotics constitute a serious health problem. This problem has led to increased interest in use bacteriophages, which have great potential as antimicrobial agents but also carry risk inducing resistance. The objective present study was minimize development phage resistance Klebsiella pneumoniae strains by inhibiting quorum sensing (QS) thus demonstrate role QS regulating defense mechanisms. Cinnamaldehyde (CAD) added K. cultures inhibit...
ABSTRACT The widespread incidence of antimicrobial resistance has created renewed interest in the use alternative treatments such as phage therapy. Phages are viruses that infect bacteria and generally have a narrow host-range. Combining phages with antibiotics can prevent emergence bacterial resistance. aim present study was to develop therapy medical products (PTMPs) targeting clinical isolates carbapenem-producing Klebsiella pneumoniae belonging high-risk clone ST512. From collection 22...
The combination of several therapeutic strategies is often seen as a good way to decrease resistance rates, since bacteria can more easily overcome single-drug treatments than multi-drug ones. This strategy especially attractive when targets and subpopulations are affected, it the case Klebsiella pneumoniae persister cells, subpopulation able transiently survive antibiotic exposures. work aims evaluate potential repurposed anticancer drug, mitomycin C, combined with K. lytic phage...
ABSTRACT Pseudomonas aeruginosa is a bacterial pathogen that major cause of lung infections in cystic fibrosis (CF) and other patients. Isolates P. from CF patients commonly carry filamentous phages (Pf phages), which constitute family temperate known to be related biofilm production antibiotic sequestration. In this study, we identified 12 new Pf phage genomes collection clinical isolates Study the anti-phage defense systems revealed presence 89 such systems, eight were encoded genomes....
Introduction Antimicrobial resistance (AMR) is a major public health threat, driving the need for alternative treatments such as phage therapy. However, bacterial defense mechanisms, often regulated by quorum sensing (QS) network and encoded in genomic islands (GIs), can generate phage-resistant mutants. Understanding these mechanisms essential optimizing Methods This study analyzed 48 Klebsiella pneumoniae strains to identify pathogenicity (PAIs) containing anti-phage (APD) proteins. We...
ABSTRACT Multidrug resistant (MDR) bacteria and the shortage of new antibiotics are a serious health problem have increased interest in bacteriophages, with great potential as antimicrobial agents but they can induce resistance. The objective present study was to reduce development phage resistance K. pneumoniae strains by inhibiting Quorum Sensing (QS). QS inhibition cinnamaldehyde (CAD) confirmed indirectly reduction biofilm production directly proteomic analysis. Also, infection assays...
Pseudomonas aeruginosa is a bacterial pathogen that major cause of lung infections in cystic fibrosis (CF) and other patients. Isolates P. from CF patients commonly carry filamentous phages (Pf phages), type temperate phage known to be related biofilm production antibiotic sequestration. In this study, 12 new Pf-like were identified collection clinical isolates Analysis the genomes revealed different anti-phage defence systems, described here for first time these types phages. Finally,...
The widespread incidence of antimicrobial resistance has created renewed interest in the use alternative treatments such as phage therapy. Phages are viruses that infect bacteria and generally have a narrow host-range. Combining phages with antibiotics can prevent emergence bacterial resistance. aim present study was to develop therapy medical products (PTMPs) targeting clinical isolates carbapenems-producing Klebsiella pneumoniae belonging high-risk clone ST512. From collection twenty-two...
ABSTRACT Antimicrobial resistance (AMR) is an important threat to public health that has led the development of innovative alternative treatments for bacterial infections, such as phage therapy. However, one greatest disadvantages therapy generation phage-resistant mutants via defence mechanisms, which are mainly contained in genomic islands (GIs) and controlled by quorum sensing (QS) network. In this study, 309 pathogenic (PAIs) harbouring a total 22.1 % proteins related anti-phage (APD)...
Abstract Background novel approaches to treat Klebsiella pneumoniae infections are desperately needed, such as the use of rationally designed combination therapies. Objectives evaluate in vitro and vivo therapeutic potential lytic phages against K. with octapeptin, a promising class lipopeptides broad spectrum Gram-negative activity. Methods we determined MICs twenty-two lipopeptide compounds chose one octapeptin (OPX10053) for evaluation synergism using checkerboard assays, optical density...
Abstract Mucins are important glycoproteins that form a protective layer throughout the gastrointestinal and respiratory tracts. There is scientific evidence of increase in phage-resistance presence mucin for some bacterial pathogens. Manipulation composition may ultimately influence effectiveness phage therapy. In this work, two clinical strains K. pneumoniae (K3574 K3325), were exposed to lytic bacteriophage vB_KpnS-VAC35 absence on long-term co-evolution assay, an attempt mimic vitro...
Mucins are important glycoproteins that form a protective layer throughout the gastrointestinal and respiratory tracts. There is scientific evidence of increase in phage-resistance presence mucin for some bacterial pathogens. Manipulation composition may ultimately influence effectiveness phage therapy. In this work, two clinical strains K. pneumoniae (K3574 K3325), were exposed to lytic bacteriophage vB_KpnS-VAC35 absence on long-term co-evolution assay, an attempt mimic vitro exposure...
ABSTRACT Lytic phages are currently considered among the best options for treating infections caused by multi-drug resistant pathogens. Phages have some advantages over conventional antibiotics. For example, acquire modifications in accordance with their environment, and thus bacteria present, which has led to co-evolution of both types organism. Therefore, acquired resistance mechanisms protection. In this context, aims present study were analyze proteins isolated from twenty-one novel...
Abstract Phage tail-like bacteriocins (PTLBs) are large proteomic structures similar to the tail phages. These function in bacterial competition by making pores membrane of their competitors. The PTLBs identified Pseudomonas pneumoniae known as R and F pyocins, which have a narrow spectrum action. Their specificity is determined fiber closely related lipopolysaccharide type target competitor strain. In this study, genome sequences 32 clinical isolates P. aeruginosa were analysed investigate...