A5080299359- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Enterobacteriaceae and Cronobacter Research
- Bacterial biofilms and quorum sensing
- Antibiotic Use and Resistance
- Pharmaceutical and Antibiotic Environmental Impacts
- Pneumonia and Respiratory Infections
- Diabetes and associated disorders
- Genomics and Phylogenetic Studies
- Diabetes Management and Research
- Urinary Tract Infections Management
- Pancreatic function and diabetes
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Infections and bacterial resistance
- Gut microbiota and health
- Microbial infections and disease research
- Spanish Linguistics and Language Studies
- Cystic Fibrosis Research Advances
- Nursing care and research
- Media, Journalism, and Communication History
- Social Sciences and Policies
- Evolution and Genetic Dynamics
Instituto Ramón y Cajal de Investigación Sanitaria
2015-2025
Hospital Universitario Ramón y Cajal
2015-2025
Centro de Investigación Biomédica en Red
2021-2025
Instituto de Salud Carlos III
2015-2025
Biomedical Research Institute of Lleida
2017-2024
Hospital Universitari Arnau de Vilanova
2017-2024
Universitat de Lleida
2024
Fundación Seimc-Gesida
2023
Red Espanola de Investigacion en Patologia Infecciosa
2015-2023
Instituto Cajal
2022-2023
Abstract Plasmid persistence in bacterial populations is strongly influenced by the fitness effects associated with plasmid carriage. However, wild-type hosts remain largely unexplored. In this study, we determined of major antibiotic resistance pOXA-48_K8 wild-type, ecologically compatible enterobacterial isolates from human gut microbiota. Our results show that although produced an overall reduction fitness, it small most hosts, and even beneficial several isolates. Moreover, genomic...
FDC susceptibility testing is challenging as none of the commercial tests have been proven to accurately determine in area technical uncertainty (ATU). Here, we evaluated performance different methods on Klebsiella pneumoniae isolates around this range. A collection 104 K. with was designed, including high representation (70%) 2024-ATU (21-23 mm) ± 1 mm. MICs were determined by broth-microdilution (BMD) reference method, BMD (ComASP®, EUMDROXF®), MIC-gradient strips (Liofilchem) and...
The ST131 Escherichia coli clone is associated with the global dissemination of ESBLs. It has been hypothesized that could take advantage better colonizing abilities. However, data on colonization prevalence ESBL-ST131 in European hospitals are scarce. To assess and its microbiological characteristics among ESBL-producing E. (ESBL-Ec) from hospitalized patients four (Berlin, Geneva, Madrid Utrecht) during R-GNOSIS study. ESBL-Ec isolates (n = 688) were obtained rectal swabs March 2014 to...
Novel β-lactam-β-lactamase inhibitor combinations currently approved for clinical use are poorly active against metallo-β-lactamase (MBL)-producing strains. We evaluated the
The emergence of Klebsiella pneumoniae isolates carrying novel blaKPC variants conferring ceftazidime-avibactam (CAZ/AVI) resistance is being increasingly reported. We evaluated the accuracy phenotypic methods commonly used in routine clinical laboratories detection K. carbapenemase (KPC) enzymes. Additionally, we characterized by whole-genome sequencing (WGS) KPC-ST307-K. recovered our hospital before and after CAZ/AVI therapy. Rectal colonization or infection carbapenem-resistant KPC-3...
Abstract Phage tail-like bacteriocins (PTLBs) are large proteomic structures similar to the tail phages. These function in bacterial competition by making pores membrane of their competitors. The PTLBs identified Pseudomonas aeruginosa known as R-type and F-type pyocins, which have a narrow spectrum action. Their specificity is determined fiber closely related lipopolysaccharide type target competitor strain. In this study, genome sequences 32 clinical P. isolates were analysed investigate...
To describe the incidence and microbiological features of carbapenemase-producing Enterobacteriaceae (CPE) from colonized patients in a Spanish university hospital during cluster-randomized study [the Resistance Gram-Negative Organisms: Studying Intervention Strategies (R-GNOSIS) project] on isolation strategies for faecal ESBL carriers.From March 2014 to 2016, 15 556 rectal swabs 8209 admitted two surgical wards medical were collected seeded CPE chromogenic agars. Carbapenemase...
Abstract Objectives Ceftazidime/avibactam and cefiderocol are two of the latest antibiotics with activity against a wide variety Gram-negatives, including carbapenem-resistant Enterobacterales. We sought to describe phenotypic genotypic characteristics ceftazidime/avibactam- cefiderocol-resistant KPC-Klebsiella pneumoniae (KPC-Kp) detected during an outbreak in 2020 medical ICU our hospital. Methods collected 11 KPC-Kp isolates (6 clinical; 5 surveillance samples) resistant...
The Enterobacter cloacae complex (ECC) encompasses heterogeneous clusters of species that have been associated with nosocomial outbreaks. These may different acquired antimicrobial resistance and virulence mechanisms, their identification is challenging. This study aims to develop predictive models based on matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) profiles machine learning for species-level identification. A total 219 ECC 118 Klebsiella...
Abstract Objectives Despite the introduction of cystic fibrosis transmembrane conductance regulator (CFTR) modulators, Pseudomonas aeruginosa is still a major pathogen in people with (pwCF). We determine activity cefiderocol and comparators collection 154 P. isolates recovered from pwCF during three multicentre studies performed 17 Spanish hospitals 2013, 2017 2021. Methods ISO broth microdilution was MICs were interpreted CLSI EUCAST criteria. Mutation frequency WGS also performed. Results...
We describe the clonal spread of New Delhi metallo-β-lactamase (NDM) 1-producing
The role of wild animals, particularly migratory birds, in the dissemination antibiotic-resistant bacteria between geographically distant ecosystems is usually underestimated. aim this work was to characterize Escherichia coli population from Franklin's gull feces, focusing on extended-spectrum β-lactamase (ESBL)-producing strains. In summer 2011, 124 fecal swabs seagulls (1 each) migrating United States and Canada coast Antofagasta, north Chile, were collected. Samples seeded MacConkey agar...
To assess the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) faecal carriers at admission in a University Hospital Spain. Prevalence survey. Pneumology, gastroenterology, urology and neurosurgery units university tertiary hospital Madrid (Spain). A total 10 643 patients aged 18 older admitted from March 2014 to April 2016 with rectal swab taken or as soon possible within first 48 hours. ESBL-E infections admission. The prevalance was 7.69% (CI 95%...
Abstract Background Murepavadin, a novel peptidomimetic antibiotic, is being developed as an inhalation therapy for treatment of Pseudomonas aeruginosa respiratory infection in people with cystic fibrosis (CF). It blocks the activity LptD protein P. causing outer membrane alterations. Objectives To determine vitro murepavadin against CF isolates and to investigate potential mechanisms resistance. Methods MIC values were determined by both broth microdilution agar dilution results compared....
The objective of this study was to describe the prevalence and microbiological characteristics carbapenemase-producing Enterobacteriaceae (CPE) colonizing patients in long-term care hospitals (LTCHs) Madrid, Spain.Three LTCHs were included a single-day point-prevalence survey (September 2013). Rectal swabs, collected from all hospitalized (137 LTCH-A, 121 LTCH-B 83 LTCH-C), plated onto chromogenic media. Population structure (PFGE MLST), genes encoding carbapenemases ESBLs plasmids carrying...
BackgroundThe current spread of carbapenemase-producing Enterobacterales (CPE) is a great concern.MethodsWe recovered 198 CPE from 162 patients admitted in our Hospital (March 2014-March 2016) during the R-GNOSIS European Project. Microbiological features and plasmid characteristics co-colonized with multiple were studied.FindingsThirty (18.5%; CI 95%= 12.5%–24.5%) presented co-colonization producing same (CPE-SC) (15.4%) or different carbapenemase (CPE-DC) (4.3%). OXA-48 (83.3%) was most...
We report the emergence of an isolate belonging to sequence type (ST)131-Escherichia coli high-risk clone with ceftazidime-avibactam resistance recovered from a patient bacteremia in 2019. Antimicrobial susceptibility was determined and whole genome sequencing (Illumina-NovaSeq6000) cloning experiments were performed investigate its phenotype. A KPC-3-producing E. susceptible (MIC = 0.5/4 mg/L) non-wild MIC meropenem (8 detected blood culture at hospital admission. Following 10-days standard...
Carbapenemase-mediated carbapenem resistance in Pseudomonas aeruginosa is a relevant health problem. We detected for the first time Spain two clinical NDM-producing P. (NDM-Pa) isolates Ukrainian patients admitted to our hospital between April-August 2022. Antimicrobial susceptibility was studied by microdilution and MIC gradient strips (EUCAST-2022 criteria). Carbapenemase genes were Xpert Carba-R immunochromatography assays. WGS (Illumina Oxford-Nanopore) also performed. In May-2022, we an...
Carbapenem-resistant pathogens have been recognized as a health concern they are both difficult to treat and detect in clinical microbiology laboratories. Researchers making great efforts develop highly specific, sensitive, accurate, rapid diagnostic techniques, required prevent the spread of these microorganisms improve prognosis patients. In this context, CRISPR-Cas systems proposed promising tools for development methods due their high specificity; Cas13a endonuclease can discriminate...
Plasmids are extrachromosomal genetic elements commonly found in bacteria. known to fuel bacterial evolution through horizontal gene transfer (HGT), but recent analyses indicate that they can also promote intragenomic adaptations. However, the role of plasmids as catalysts beyond HGT remains poorly explored. In this study, we investigate impact a widespread conjugative plasmid, pOXA-48, on various multidrug-resistant clinical enterobacteria. Combining experimental and within-patient...
Carbapenemase-producing Enterobacterales (CPE) are increasingly recognized in nosocomial infections, also affecting ICU patients. We aimed to characterize the carbapenemase-producing Serratia marcescens (CPSm) isolates recovered our hospital Madrid (Spain) between March 2016 and December 2018.Overall, 50 from clinical epidemiological surveillance samples were 24 patients admitted medical 10 non-ICU-related based on their phenotypic resistance. Carbapenemase characterization, antibiotic...