A5065792523- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Evolution and Genetic Dynamics
- Microbial infections and disease research
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- SARS-CoV-2 detection and testing
- Polyomavirus and related diseases
- Antibiotic Resistance in Bacteria
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- Genetics, Bioinformatics, and Biomedical Research
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 Clinical Research Studies
- Mathematical and Theoretical Epidemiology and Ecology Models
- Nanoparticles: synthesis and applications
- HIV Research and Treatment
- Advanced Nanomaterials in Catalysis
- Hepatitis B Virus Studies
- Plant and Fungal Interactions Research
- Animal Virus Infections Studies
- Vibrio bacteria research studies
Institute for Integrative Systems Biology
2018-2025
Universitat de València
2013-2025
Consejo Superior de Investigaciones Científicas
2019-2024
Parc Científic de la Universitat de València
2020-2024
National Research Council
2022
Université de Strasbourg
2014-2018
Inserm
2014-2018
Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine
2016-2018
Architecture et Réactivité de l'arN
2015
Institut de Biologie Moléculaire et Cellulaire
2015
Bacteriophages play key roles in bacterial ecology and evolution are potential antimicrobials. However, the determinants of phage-host specificity remain elusive. Here, we isolate 46 phages to challenge 138 representative clinical isolates Klebsiella pneumoniae, a widespread opportunistic pathogen. Spot tests show narrow host range for most phages, with <2% 6,319 combinations tested yielding detectable interactions. Bacterial capsule diversity is main factor restricting phage range....
Mutational fitness effects can be measured with relatively high accuracy in viruses due to their small genome size, which facilitates full-length sequencing and genetic manipulation. Previous work has shown that animal plant RNA are very sensitive mutation. Here, we characterize mutational single-stranded (ss) DNA ssRNA bacterial viruses. First, performed a mutation-accumulation experiment subjected three ssDNA (ΦX174, G4, F1) phages (Qβ, MS2, SP) plaque-to-plaque transfers chemical...
BK virus–associated nephropathy (BKVAN) causes renal allograft dysfunction. The current management of BKVAN relies on pre-emptive adaptation immunosuppression according to viral load monitoring. However, this empiric strategy is not always successful. Therefore, pretransplant predictive markers are needed. In a prospective longitudinal study, we enrolled 168 kidney transplant recipients and 69 matched donors. To assess the value BKV genotype–specific neutralizing antibody (NAb) titers as...
Abstract Phages are increasingly considered promising alternatives to target drug-resistant bacterial pathogens. However, their often-narrow host range can make it challenging find matching phages against bacteria of interest. Current computational tools do not accurately predict interactions at the strain level in a way that is relevant and properly evaluated for practical use. We present PhageHostLearn, machine learning system predicts strain-level between receptor-binding proteins...
Abstract Bacterial evolution is affected by mobile genetic elements like phages and conjugative plasmids, offering new adaptive traits while incurring fitness costs. Their infection the bacterial capsule. Yet, its importance has been difficult to quantify because of high diversity confounding mechanisms in genomes such as anti-viral systems surface receptor modifications. Swapping capsule loci between Klebsiella pneumoniae strains allowed us their impact on plasmid phage independently...
Abstract Background The COVID-19 disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a rapidly emerging pandemic which has enforced extreme containment measures worldwide. In the absence of vaccine or efficient treatment, cost-effective epidemiological surveillance strategies are urgently needed. Methods Here, we have used RT-qPCR for SARS-CoV-2 detection in series longitudinal metropolitan wastewaters samples collected during earliest stages epidemic Region...
Klebsiella pneumoniae is an opportunistic Gram-negative pathogen that employs different strategies (resistance and persistence) to counteract antibiotic treatments. This study aimed search for new means of combatting imipenem-resistant persister strains K. by repurposing the anticancer drug mitomycin C as antimicrobial agent combining conventional imipenem with lytic phage vB_KpnM-VAC13. Several clinical isolates were characterized, isolate (harboring OXA-245 β-lactamase) a selected study....
In this work, nanostructured copper materials have been designed, synthetized, and evaluated in order to produce a more efficient sustainable bionanohybrid with catalytical antimicrobial properties. Thus, conditions are sought where the most critical steps reduced or minimized, such as use of reducing agents cryogenization step. addition, new characterized through different techniques, their oxidative reductive capacities, well activity, evaluated. The addition quantities agent synthesis...
is a bacterium that can cause food-borne infections and responsible for the most common gastrointestinal illnesses. The emergence of multi-drug resistant (MDR) strains worldwide major threat, representing challenge in public health. To reduce its incidence, One Health approach required, development new biocontrol protocols will help prevent or eliminate spread
Genome sizes and mutation rates covary across all domains of life. In unicellular organisms DNA viruses, they show an inverse relationship known as Drake's rule. However, it is still unclear whether a similar exists between genome in RNA genomes. Coronaviruses, the viruses with largest genomes (∼30 kb), encode proofreading 3' exonuclease that allows them to increase replication fidelity. unknown whether, conversely, smallest tend particularly high rates. To test this, we measured rate...
The SARS-CoV-2 can be excreted in feces and reach sewage systems. Determining the presence of infective viral particles is necessary to take adequate control measures elucidate new routes transmission. Here, we have developed a sample concentration methodology that allows us maintain infectivity. Feces COVID-19 patients wastewater samples been analyzed both by molecular methods cell culture. Our results show no evidence particles, suggesting fecal-oral transmission not primary route....
Abstract Since their discovery, toxin-antitoxin (TA) systems have captivated the attention of many scientists. Recent studies demonstrated that TA play a key role in phage inhibition. The aim present study was to investigate PemIK (PemK/PemI) type II system inhibition by its intrinsic expression clinical strains Klebsiella pneumoniae carrying lncL plasmid, which harbours carbapenemase OXA-48 and PemK/PemI system. Furthermore, induced an IPTG-inducible plasmid reference strain K. ATCC10031...