A5077880593- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Polyomavirus and related diseases
- SARS-CoV-2 and COVID-19 Research
- Parvovirus B19 Infection Studies
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Monoclonal and Polyclonal Antibodies Research
- Plant Virus Research Studies
- Energy Harvesting in Wireless Networks
- Full-Duplex Wireless Communications
- Mosquito-borne diseases and control
- Stress Responses and Cortisol
- HIV Research and Treatment
- COVID-19 Impact on Reproduction
- Antenna Design and Analysis
- Viral Infections and Immunology Research
- Systemic Lupus Erythematosus Research
- Tryptophan and brain disorders
- Cytomegalovirus and herpesvirus research
- Diabetes and associated disorders
- Vector-borne infectious diseases
- Bipolar Disorder and Treatment
- T-cell and B-cell Immunology
Université de Strasbourg
2010-2022
Inserm
2010-2022
Institut de Virologie
2006-2022
Immuno-Rhumathologie moléculaire
2022
Hôpitaux Universitaires de Strasbourg
2008-2016
Eurométropole de Strasbourg
2007
Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Scavenger receptor class B type I (SR-BI) has been shown to bind HCV envelope glycoprotein E2, participate in entry pseudotype particles, and modulate infection. However, the functional role SR-BI for productive infection remains unclear. In this study, we investigated as an factor human hepatoma cells using cell culture-derived (HCVcc). Anti-SR-BI antibodies directed against epitopes extracellular loop specifically...
The tight junction protein claudin-1 (CLDN1) has been shown to be essential for hepatitis C virus (HCV) entry—the first step of viral infection. Due the lack neutralizing anti-CLDN1 antibodies, role CLDN1 in entry process is poorly understood. In this study, we produced antibodies directed against human extracellular loops by genetic immunization and used these investigate mechanistic HCV an infectious cell culture system hepatocytes. Antibodies specific surface–expressed specifically...
End-stage liver disease caused by chronic hepatitis C virus (HCV) infection is a leading cause for transplantation (LT). Due to viral evasion from host immune responses and the absence of preventive antiviral strategies, reinfection graft universal. The mechanisms which evades immunity reinfect are unknown. In longitudinal analysis six HCV-infected patients undergoing LT, we demonstrate that HCV variants reinfecting were characterized efficient entry poor neutralization antibodies present in...
Hepatitis C virus (HCV) is transmitted between hepatocytes via classical cell entry but also uses direct cell-cell transfer to infect neighboring hepatocytes. Viral transmission has been shown play an important role in viral persistence allowing evasion from neutralizing antibodies. In contrast, the of HCV for antiviral resistance unknown. Aiming address this question we investigated phenotype strains exhibiting direct-acting antivirals (DAAs) state-of-the-art model systems and spread. Using...
BK virus–associated nephropathy (BKVAN) causes renal allograft dysfunction. The current management of BKVAN relies on pre-emptive adaptation immunosuppression according to viral load monitoring. However, this empiric strategy is not always successful. Therefore, pretransplant predictive markers are needed. In a prospective longitudinal study, we enrolled 168 kidney transplant recipients and 69 matched donors. To assess the value BKV genotype–specific neutralizing antibody (NAb) titers as...
Transplant recipients, who receive therapeutic immunosuppression to prevent graft rejection, are characterized by high coronavirus disease 2019 (COVID-19)-related mortality and defective response vaccines. We observed that previous infection with severe acute respiratory syndrome 2 (SARS-CoV-2), but not the standard two-dose regimen of vaccination, provided protection against symptomatic COVID-19 in kidney transplant recipients. therefore compared cellular humoral immune responses these two...
BK virus (BKV) replication occurs frequently in kidney transplant recipients (KTR), potentially leading to BKV-associated nephropathy (BKVAN) and graft loss. Patients with high titers of BKV-neutralizing antibodies (NAbs) are protected against BKV replication, intravenous immunoglobulin (IVIg) infusion can increase NAb titers. We investigated whether early IVIg administration prevents patients low (<4 log10 the BKV-specific genotype). Based on day transplantation, KTR followed Strasbourg...
In patients with hepatitis C virus (HCV) infection, enhanced activity of indoleamine-2,3-dioxygenase 1 (IDO) has been reported. IDO - a tryptophan-catabolizing enzyme considered as both an innate defence mechanism and important regulator the immune response. The molecular induction in HCV infection its role antiviral response remain unknown. Using primary human hepatocytes, we show that stimulates expression. gene was transient coincided expression types I III interferons (IFNs)...
International guidelines define a BK virus (BKV) load of ≥4 log10 copies/ml as presumptive BKV-associated nephropathy (BKVN) and cutoff for therapeutic intervention. To investigate whether BKV DNA loads (BKVL) are comparable between laboratories, 2 panels 15 8 clinical specimens (urine, whole blood, plasma) harboring different genotypes were distributed to 20 27 French hospital centers in 2013 2014, respectively. Although 68% the reported results fell within acceptable range expected result...
BK polyomavirus (BKPyV)-associated nephropathy (BKPyVAN) is one of the major causes kidney graft dysfunction, and there are no BKPyV-specific antiviral therapies available. BKPyV neutralizing antibodies (NAbs) play key roles in protecting against replication represent a potential therapeutic or preventive strategy. In this study, we evaluated NAb titers intravenous immunoglobulin (i.v. Ig) preparations transplant recipients (KTR) before after i.v.
End-stage liver disease as a result of chronic hepatitis C virus (HCV) infection is the main indication for transplant (LT), but allografts are systematically infected with HCV soon after transplant. Viral quasispecies poorly described during early posttransplant period.For 17 patients who received an LT disease, plasma viral evolution was determined by sequence analysis hypervariable region 1 E2 envelope gene before (BT), 7 days (D7), and month (M1). T helper (Th)1/Th2 cytokine levels were...
Nonrandom distribution of hepatitis C virus (HCV) quasispecies (compartmentalization between blood plasma and leukocytes) suggests the presence HCV leukotropic variants. compartmentalization in setting liver transplantation (LT) is poorly understood. To study variants, we investigated evolution after immunosuppression transplant recipients.Plasma peripheral mononuclear cell (PBMC) samples were collected from 5 recipients before LT. We used clone sequencing to analyze hypervariable region 1...
Screening of BK virus (BKV) replication is recommended to identify patients at increased risk BKV-associated diseases. However, the heterogeneity molecular techniques hinders establishment universal guidelines for BKV monitoring. Here we aimed compare performance CE-marked R-gene kit (R-gene) our in-house assay quantification DNA loads (BKVL). A 12-specimen panel from Quality Control Molecular Diagnostics (QCMD) organization, 163 urine samples, and 88 paired specimens plasma whole blood (WB)...
Abstract Transplant recipients, which receive therapeutic immunosuppression to prevent graft rejection, are characterized by high COVID-19-related mortality and defective response vaccines. Having observed that previous infection SARS-CoV-2 but not the standard “2 doses” scheme of vaccination, provided complete protection against COVID-19 transplant we undertook this translational study compare cellular humoral immune responses these 2 groups patients. Neutralizing anti-Receptor Binding...
Background SARS-CoV-2 breakthrough infections after complete vaccination are increasing whereas their determinants remain uncharacterized. Methods We analyzed two cases of post-vaccination by α and β variants, respectively. For each participant both humoral (binding neutralizing antibodies) cellular (activation markers cytokine expression) immune responses were characterized longitudinally. Results The first (P1) was infected an variant displayed extended short period viral excretion...
Cirrhosis and hepatocarcinoma related to hepatitis C virus (HCV) lead more than 30% of liver transplantations. Host- virus-related mechanisms, involved in the recurrence HCV infection graft, are not yet well known. A weak CD4+ T-cell response was shown be outcome re-infection but whether dendritic cell numbers modified patients transplanted for HCV-related disease has never been evaluated. Eight eight non-HCV-infected controls were included. Blood plasmacytoid cells myeloid quantified before...
JC virus (JCV) causes progressive multifocal leukoencephalopathy (PML) in immunosuppressed patients. There is currently no effective specific antiviral treatment and PML management relies on immune restoration. Prognosis markers are crucially needed this disease because of its high mortality rate. In work, we investigated the compartmentalization JCV strains as well humoral neutralizing response various matrices to further understand pathophysiology define survival. Four patients were...
As a consequence of selective pressure exerted by the immune response during hepatitis C virus (HCV) infection, high rate nucleotide mutations in viral genome is observed which leads to emergence escape mutants. The aim this study was evaluate evolution amino acid (aa) sequence HCV nonstructural protein 3 (NS3) isolates after liver transplantation. Six patients with HCV-induced disease undergoing transplantation (LT) were followed up for analysis. Hepatitis recurrence all LT. synonymous (dS)...