A5081168031- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Hepatitis Viruses Studies and Epidemiology
- Salmonella and Campylobacter epidemiology
- Respiratory viral infections research
- Environmental Justice and Health Disparities
- Microplastics and Plastic Pollution
- Animal Disease Management and Epidemiology
- Municipal Solid Waste Management
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- Plant Virus Research Studies
- Recycling and Waste Management Techniques
- COVID-19 diagnosis using AI
- Fecal contamination and water quality
- COVID-19 Clinical Research Studies
- Veterinary medicine and infectious diseases
- Microbial Community Ecology and Physiology
- Advanced Biosensing Techniques and Applications
- Infection Control and Ventilation
- Cancer Research and Treatments
Instituto de Agroquímica y Tecnología de Alimentos
2020-2025
INCLIVA Health Research Institute
2025
Consejo Superior de Investigaciones Científicas
2020-2022
Universitat de Barcelona
2020-2022
Wastewater-based epidemiology (WBE) has lately arised as a promising tool for monitoring and tracking viral pathogens in communities. In this study, we analysed WBE's role multi-pathogen surveillance strategy to detect the presence of several illness causative agents. Thus, an epidemiological study was conducted from October 2021 February 2023 estimate weekly levels Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Syncytial virus (RSV), Influenza A (IAV) influent wastewater...
Abstract Background The COVID-19 disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a rapidly emerging pandemic which has enforced extreme containment measures worldwide. In the absence of vaccine or efficient treatment, cost-effective epidemiological surveillance strategies are urgently needed. Methods Here, we have used RT-qPCR for SARS-CoV-2 detection in series longitudinal metropolitan wastewaters samples collected during earliest stages epidemic Region...
Hepatitis E virus (HEV), particularly zoonotic genotype 3, is present in environmental waters worldwide, especially industrialized countries. Thus, monitoring the presence of HEV wastewater treatment plants (WWTPs) an emerging topic due to importance reusing water on a global level. Given limited data, this study aimed monitor occurrence influent and effluent waste- drinking- (WWTPs DWTPs). To end, different procedures concentrate from WWTPs DWTPs were initially evaluated. The evaluated...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 200,000 reported COVID-19 cases in Spain resulting 20,800 deaths as of April 21, 2020. Faecal shedding SARS-CoV-2 RNA from patients extensively been reported. Therefore, we investigated the occurrence six wastewater treatments plants (WWTPs) serving major municipalities within Region Murcia (Spain), area with lowest prevalence Iberian Peninsula. Firstly, an aluminum hydroxide adsorption-precipitation...
Wastewater discharge to the environment or its reuse after sanitization poses a concern for public health given risk of transmission human viral diseases. However, estimating infectivity along wastewater cycle presents technical challenges and still remains underexplored. Recently, human-associated crAssphage has been investigated serve as pathogen indicator monitor fecal impacted water bodies, even though assessment biomarker infectious enteric viruses not explored yet. To this end,...
During the current COVID-19 pandemic, wastewater-based epidemiology (WBE) emerged as a reliable strategy both surveillance method and way to provide an overview of SARS-CoV-2 variants circulating among population. Our objective was compare two different concentration methods, well-established aluminum-based procedure (AP) commercially available Maxwell® RSC Enviro Wastewater TNA Kit (TNA) for human enteric virus, viral indicators surveillance. Additionally, methods were analyzed their impact...
Abstract Isolation, contact tracing and restrictions on social movement are being globally implemented to prevent control onward spread of SARS-CoV-2, even though the infection risk modelled RNA detection by RT-qPCR remains biased as viral shedding infectivity not discerned. Thus, we aimed develop a rapid viability procedure infer SARS-CoV-2 in clinical specimens environmental samples. We screened monoazide dyes platinum compounds molecular markers five targets. A chloride-based was then...
ABSTRACT We investigated whether antibody concentrations measured in plasma using the Roche Elecsys® Anti‐SARS‐CoV‐2 S assay (targeting receptor binding domain, RBD) could estimate levels of Wuhan‐Hu‐1 and Omicron XBB.1.5 spike‐directed antibodies with neutralizing ability (NtAb) or those mediating NK‐cell activity. analyzed 135 samples from 39 vaccinated elderly nursing home residents. A strong correlation was found for NtAb against both (Rho = 0.73, p < 0.001) (sub)variants 0.001)....
Los virus de la influenza A (IAV) representan una amenaza significativa para salud humana y animal. Pese a las medidas control, diferentes brotes continúan produciéndose con riesgo transmisión zoonótica. Recientes en vacas lecheras EE. UU. variante H5N1 subrayan necesidad métodos efectivos detección. En este estudio, se evaluó el método adsorción-precipitación por aluminio (AlCl3) tipos leche (cruda, pasteurizada UHT) detección IAV. La extracción ácidos nucleicos realizó utilizando equipo...
The use of SARS-CoV-2 metagenomics in wastewater can allow the detection variants circulating at community level. After comparing with clinical databases, we identified three novel spike gene, and six new detected for first time Spain. We finally support hypothesis that this approach allows identification unknown or only low frequencies genomes. Abstract Figure
Abstract This year-long research analyzed emerging risks in influent, effluent wastewaters and biosolids from six wastewater treatment plants Spain’s Valencian Region. Specifically, it focused on human enteric respiratory viruses, bacterial viral faecal contamination indicators, extended-spectrum beta-lactamases-producing Escherichia coli , antibiotic-resistance genes. Additionally, particles microplastics biosolid samples were assessed. Human viruses prevalent influent wastewater, with...
Abstract Combined kinetic analysis of plasma SARS-CoV-2 RNAemia, Nucleocapsid (N)-antigenemia and virus-specific antibodies may help ascertain the role in preventing virus dissemination COVID-19 patients. We performed this a cohort 71 consecutive critically ill patients (49 male; median age, 65 years) using RT-PCR assay, lateral flow immunochromatography method receptor binding domain (RBD) N-based immunoassays. A total 338 specimens collected at 12 days after symptoms onset were available...
The ongoing coronavirus 2019 (COVID-19) pandemic constitutes a concerning global threat to public health and economy. In the midst of this scenario, role environment-to-human COVID-19 spread is still matter debate because mixed results have been reported SARS-CoV-2 stability on high-touch surfaces in real-life scenarios. Up now, no alternative accessible procedures for cell culture applied evaluate infectivity fomites. Several strategies based viral capsid integrity latterly developed using...
Background: The COVID-19 disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a rapidly emerging pandemic which has enforced extreme containment measures worldwide. In the absence of vaccine or efficient treatment, cost-effective epidemiological surveillance strategies are urgently needed. Methods: Here, we have used RT-qPCR for SARS-CoV-2 detection in series longitudinal metropolitan wastewaters samples collected during earliest stages epidemic Region...
The virological quality of process water (PW) used by the produce industry has received limited attention. As a first step to overcoming technical limitations in monitoring viruses PW, analytical performance ultrafiltration was assessed concentrate viral particles from 20 L spiked PW. selected method for sample concentration PW carefully validated, thus enabling accurate quantification and estimation titers human enteric phages. collected periodically washing tanks commercial facilities....