A5061946610- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Energy Harvesting in Wireless Networks
- Infant Nutrition and Health
- Clostridium difficile and Clostridium perfringens research
- Respiratory viral infections research
- Cell Adhesion Molecules Research
- Escherichia coli research studies
- Cytomegalovirus and herpesvirus research
- Hepatitis Viruses Studies and Epidemiology
- Congenital Anomalies and Fetal Surgery
- Digestive system and related health
- Advanced Thermodynamic Systems and Engines
- Long-Term Effects of COVID-19
- Protease and Inhibitor Mechanisms
- Hematopoietic Stem Cell Transplantation
- Monoclonal and Polyclonal Antibodies Research
- Gut microbiota and health
- Immune Cell Function and Interaction
Universitat de València
2016-2025
INCLIVA Health Research Institute
2018-2025
Hospital Clínico Universitario de Valencia
2021-2024
Instituto de Biomedicina de Valencia
2021-2022
Instituto de Agroquímica y Tecnología de Alimentos
2012-2021
Centro de Investigacion Principe Felipe
2010-2020
Parc Científic de la Universitat de València
2018
The gut microbiota has emerged as a key factor in the pathogenesis of intestinal viruses, including enteroviruses, noroviruses and rotaviruses (RVs), where stimulatory inhibitory effects on infectivity have been reported. With aim determining whether members interact with RVs during infection, combination anti-RV antibody labeling, fluorescence-activated cell sorting 16S rRNA amplicon sequencing was used to characterize interaction between specific bacteria RV stool samples children...
Host genetic factors, such as histo-blood group antigens (HBGAs), are associated with susceptibility to norovirus (NoV) and rotavirus (RV) infections. Recent advances point the gut microbiome a key player necessary for viral pathogen cause infection. In vitro NoV attachment host cells resulting infections have been linked interactions certain bacterial types in microbiota. We investigated relationship between genotype, microbiota, Saliva fecal samples from 35 adult volunteers were analysed...
Viral infections, particularly human norovirus (NoV), are a leading cause of diarrheal diseases globally. To better understand NoV susceptibility, it is crucial to investigate both host glycobiology and the influence microbiota. Histo-blood group antigens (HBGA) displayed on surfaces cells act as receptors, while certain bacteria express HBGA-like substances, facilitating virus-bacteria interactions. identify bacterial species interacting with during infection, stool samples from children...
The aim of the present study was to perform molecular epidemiology rotaviruses and noroviruses detected in sewage samples from a large wastewater facility city Valencia, Spain. A total 46 were collected over one-year period (September 2016 September 2017). Norovirus rotavirus quantified by RT-qPCR, genotyped semi-nested RT-PCR further characterized sequencing phylogenetic analyses. Noroviruses widely distributed (69.6% for norovirus GI, 76.0% GII, 71.7% rotaviruses) viral loads varied 4.33...
Whether antibody levels measured by commercially available enzyme or chemiluminescent immunoassays targeting the SARS-CoV-2 spike (S) protein can act as a proxy for serum neutralizing activity remains to be established many of these assays. We evaluated degree correlation between antibodies (NtAb) binding and SARS-CoV-2-S-IgG four commercial in sera drawn from hospitalized COVID-19 patients. Ninety 51 patients were tested pseudotyped virus neutralization assay, LIAISON S1/S2 IgG, Euroimmun...
Rotavirus is the leading agent causing acute gastroenteritis in young children, with P[8] genotype accounting for more than 80% of infections humans. The molecular bases binding VP8* domain from VP4 spike protein to its cellular receptor, secretory H type-1 antigen (Fuc-α1,2-Gal-β1,3-GlcNAc; H1), and precursor lacto-N-biose (Gal-β1,3-GlcNAc; LNB) have been determined. resolution crystal structures complex H1 LNB site-directed mutagenesis experiments revealed that both glycans bind through a...
The S:A222V point mutation, within the G clade, was characteristic of 20E (EU1) SARS-CoV-2 variant identified in Spain early summer 2020. This mutation has since reappeared Delta subvariant AY.4.2, raising questions about its specific effect on viral infection. We report combined serological, functional, structural and computational studies characterizing impact this mutation. Our results reveal that promotes an increased RBD opening slightly increases ACE2 binding as compared to parent...
The gastrointestinal microbiota members produce α-l-fucosidases that play key roles in mucosal, human milk, and dietary oligosaccharide assimilation. Here, 36 open reading frames (ORFs) coding for putative belonging to glycosyl hydrolase family 29 (GH29) were identified through metagenome analysis of breast-fed infant fecal microbiome. Twenty-two those ORFs showed a complete sequence with deduced amino acid sequences displaying the highest degree identity from Bacteroides thetaiotaomicron,...
Goodpasture antigen-binding protein (GPBP) is an exportable1 Ser/Thr kinase that induces collagen IV expansion and has been associated with chemoresistance following epithelial-to-mesenchymal transition (EMT). Here we demonstrate cancer EMT phenotypes secrete GPBP (mesenchymal GPBP) which displays a predominant multimeric oligomerization directs the formation of previously unrecognized mesh networks IV). Yeast two-hybrid (YTH) system was used to identify 260SHCIE264 motif critical for...
Human milk glycans present a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development. A humanized mouse model generated fecal transplantation was utilized here to evaluate impact fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on microbiota host–microbiota interactions. 16S rRNA amplicon sequencing showed certain bacterial genera significantly increased (Ruminococcus Oscillospira) or...
Abstract The current study aimed at characterizing the dynamics of SARS‐CoV‐2 nucleocapsid (N) antigenemia in a cohort critically ill adult COVID‐19 patients and assessing its potential association with plasma levels biomarkers clinical severity mortality. Seventy‐three consecutive (median age, 65 years) were recruited. Serial ( n = 340) specimens collected. A lateral flow immunochromatography assay reverse‐transcription polymerase chain reaction (RT‐PCR) used for N protein detection RNA...
Cytomegalovirus (CMV) DNA in plasma is mainly unprotected and highly fragmented. The size of the amplicon largely explains variation CMV loads quantified across PCR platforms. In this proof-of-concept study, we assessed whether fragmentation profile may vary allogeneic hematopoietic stem cell transplant recipients (allo-SCT), within same patient over time, or affected by letermovir (LMV) use. A total 52 specimens from 14 nonconsecutive allo-SCT were included. RealTime (Abbott Molecular), was...
Enveloped viruses enter cells by binding to receptors present on host cell membranes, which trigger internalization and membrane fusion. For many viruses, this either directly or indirectly involves interaction with membrane-anchored carbohydrates, such as heparan sulfate, providing a potential target for broad-spectrum antiviral approach. Based hypothesis, we screened library of functionalized chitosan sulfates that mimic sulfate in cellular membranes inhibition SARS-CoV-2 respiratory...
Rotaviruses (RVs) are the main cause of viral diarrhea among infants, small children, and young many animal species. Histo-blood group antigens (HBGAs) potential RV receptors glycan composition on mucous surfaces influences host susceptibility cross-species virus transmission. RVs exhibit genotype-dependent binding differences due to sequence modifications in VP8* domain spike protein VP4. Nevertheless, molecular bases for this specificity, especially non-A RVs, not thoroughly understood....
Human noroviruses (NoVs) are the main etiological agents of acute gastroenteritis worldwide. While NoVs highly diverse (more than 30 genotypes have been detected in humans), during last 40 years most outbreaks and epidemics caused by GII.4 genotype strains, raising questions about their persistence population. Among other potential explanations, immune evasion is considered to be a driver success. In order study antibody recognition detail, we analyzed conformational epitope recognized...
The gastrointestinal tract accommodates more than 10 14 microorganisms that have an enormous impact on human health. mechanisms enabling commensal bacteria and administered probiotics to colonize the gut remain largely unknown. ability utilize host-derived carbon energy resources available at mucosal surfaces may provide these with a competitive advantage in gut. Here, we identified species Lactobacillus casei novel metabolic pathway for utilization of glycoamino acid fucosyl-α-1,6- N...
The immune effector mechanisms involved in protecting against severe COVID-19 infection elderly nursing home residents following vaccination or natural are not well understood. Here, we measured SARS-CoV-2 Spike (S)-directed functional antibody responses, including neutralizing antibodies (NtAb) and Fc-mediated NK cell activity (degranulation IFNγ production), the Wuhan-Hu-1, BA.4/5 (for NtAb), Omicron XBB.1.5 variants (n = 39; median age, 91 years) before a third (pre- post-3D) fourth...
Rotavirus remains a significant public health threat, especially in low-income countries, where it is the leading cause of severe acute childhood gastroenteritis, contributing to over 128,500 deaths annually. Although introduction Rotarix and RotaTeq vaccines 2006 marked milestone reducing mortality rates, approximately 83,158 preventable persisted, showing ongoing challenges vaccine accessibility effectiveness. To address these issues, novel subcutaneous formulation targeting multiple...