A5089283763- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Enzyme Structure and Function
- Microbial Metabolites in Food Biotechnology
- Infant Nutrition and Health
- Clostridium difficile and Clostridium perfringens research
- Digestive system and related health
- Gut microbiota and health
- Arsenic contamination and mitigation
- Amino Acid Enzymes and Metabolism
- Enzyme Production and Characterization
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Mercury impact and mitigation studies
- Protein Hydrolysis and Bioactive Peptides
- Heavy Metal Exposure and Toxicity
- Phytase and its Applications
- Microbial Metabolic Engineering and Bioproduction
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Biofuel production and bioconversion
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
Instituto de Agroquímica y Tecnología de Alimentos
2016-2025
Consejo Superior de Investigaciones Científicas
2008-2023
Universidad Cardenal Herrera CEU
2013
Instituto de Biomedicina de Valencia
2013
Agrobiotechnology Institute
2013
Universitat Autònoma de Barcelona
2013
Instituto Valenciano de Investigaciones Agrarias
2013
Indiana Athletic Trainers Association
2010
Centre National de la Recherche Scientifique
2000-2007
AgroParisTech
2007
The gut microbiota has emerged as a key factor in the pathogenesis of intestinal viruses, including enteroviruses, noroviruses and rotaviruses (RVs), where stimulatory inhibitory effects on infectivity have been reported. With aim determining whether members interact with RVs during infection, combination anti-RV antibody labeling, fluorescence-activated cell sorting 16S rRNA amplicon sequencing was used to characterize interaction between specific bacteria RV stool samples children...
The entire genome of Lactobacillus casei BL23, a strain with probiotic properties, has been sequenced. genomes BL23 and the industrially used Shirota YIT 9029 (Yakult) seem to be very similar.
Host genetic factors, such as histo-blood group antigens (HBGAs), are associated with susceptibility to norovirus (NoV) and rotavirus (RV) infections. Recent advances point the gut microbiome a key player necessary for viral pathogen cause infection. In vitro NoV attachment host cells resulting infections have been linked interactions certain bacterial types in microbiota. We investigated relationship between genotype, microbiota, Saliva fecal samples from 35 adult volunteers were analysed...
A novel strain of the genus Holzapfeliella, named He02T, was isolated from flowers Satureja montana L. in a survey for lactic acid bacteria associated with wild and cultivated plants metropolitan area Valencia, Spain. Partial 16S rRNA gene sequencing revealed similarity 99% to Holzapfeliella floricola DSM 23037T=Ryu1-2T. Strain He02T cells are Gram-stain-positive, catalase-negative non-motile rods, usually occurring pairs. Cells show pale yellow pigmentation when pelleted. As H. floricola,...
Viral infections, particularly human norovirus (NoV), are a leading cause of diarrheal diseases globally. To better understand NoV susceptibility, it is crucial to investigate both host glycobiology and the influence microbiota. Histo-blood group antigens (HBGA) displayed on surfaces cells act as receptors, while certain bacteria express HBGA-like substances, facilitating virus-bacteria interactions. identify bacterial species interacting with during infection, stool samples from children...
The genomes of <i>Lactobacillus casei/paracasei </i>and rhamnosus</i> strains carry two genes encoding homologues p40 and p75 from <i>L. GG, secreted proteins which display anti-apoptotic cell protective effects on human intestinal epithelial cells. cysteine, histidine-dependent aminohydrolase/peptidase (CHAP) NLPC/P60 domains, respectively, are characteristic with cell-wall hydrolase activity. In casei</i> BL23 both were to the growth medium also located at...
Three putative α-L-fucosidases encoded in the Lactobacillus casei BL23 genome were cloned and purified. The proteins displayed different abilities to hydrolyze natural fucosyloligosaccharides like 2'-fucosyllactose, H antigen disaccharide, type II trisaccharide, 3'-, 4'-, 6'-fucosyl-GlcNAc. This indicated a possible role utilization of oligosaccharides present human milk intestinal mucosa.
The predominant form of life for microorganisms in their natural habitats is the biofilm mode growth. adherence and colonization probiotic bacteria are considered as essential factors immunoregulatory function host. Here, we show that Lactobacillus casei ATCC334 adheres to colonizes gut zebrafish larvae. abundance pro-inflammatory cytokines recruitment macrophages were low when inflammation was induced probiotic-fed animals, suggesting these have anti-inflammatory properties. We treated...
ABSTRACT Polyphosphate (poly-P) is a polymer of phosphate residues synthesized and in some cases accumulated by microorganisms, where it plays crucial physiological roles such as the participation response to nutritional stringencies environmental stresses. Poly-P metabolism has received little attention Lactobacillus , genus lactic acid bacteria relevance for food production health humans animals. We show that among 34 strains 18 them intracellular poly-P granules, revealed specific...
Summary The probiotic L actobacillus casei catabolizes galacto‐ N ‐biose ( GNB ) and lacto‐ LNB by using a transport system metabolic routes different from those of B ifidobacterium . contains gene cluster, gnbREFGBCDA , involved in the metabolism also ‐acetylgalactosamine. Inactivation gnbC EIIC or ptsI (Enzyme I phosphoenolpyruvate : sugar phosphotransferase PTS prevented growth on three carbohydrates, indicating that they are transported phosphorylated same Gnb Enzyme activities analysis...
In most Gram-positive bacteria, serine-46-phosphorylated HPr (P-Ser-HPr) controls the expression of numerous catabolic genes (≈10% their genome) by acting as catabolite corepressor. kinase/phosphorylase (HprK/P), bifunctional sensor enzyme for repression, phosphorylates HPr, a phosphocarrier protein sugar-transporting phosphoenolpyruvate/glycose phosphotransferase system, in presence ATP and fructose-1,6-bisphosphate but dephosphorylates P-Ser-HPr when phosphate prevails over...
We have cloned and sequenced the Lactobacillus casei ptsH ptsI genes, which encode enzyme I HPr, respectively, general components of phosphoenolpyruvate–carbohydrate phosphotransferase system (PTS). Northern blot analysis revealed that these two genes are organized in a single‐transcriptional unit whose expression is partially induced. The PTS plays an important role sugar transport L. , as was confirmed by constructing I‐deficient mutants, were unable to ferment large number carbohydrates...
L-Fucose is a sugar present in human secretions as part of milk oligosaccharides, mucins, and other glycoconjugates the intestinal epithelium. The genome probiotic Lactobacillus rhamnosus GG (LGG) carries gene cluster encoding putative L-fucose permease (fucP), catabolic pathway (fucI, fucK, fucU, fucA), transcriptional regulator (fucR). metabolism LGG results 1,2-propanediol production, their fucI fucP mutants displayed severe mild growth defect on L-fucose, respectively. Transcriptional...
Rotavirus is the leading agent causing acute gastroenteritis in young children, with P[8] genotype accounting for more than 80% of infections humans. The molecular bases binding VP8* domain from VP4 spike protein to its cellular receptor, secretory H type-1 antigen (Fuc-α1,2-Gal-β1,3-GlcNAc; H1), and precursor lacto-N-biose (Gal-β1,3-GlcNAc; LNB) have been determined. resolution crystal structures complex H1 LNB site-directed mutagenesis experiments revealed that both glycans bind through a...
A bicameral model consisting of Caco-2 and HT29-MTX intestinal epithelial cells THP-1-derived macrophages has been used to test the ability two strains Lactobacillus protect from damage caused by mercury. Exposure 1 mg/ml mercury [Hg(II) or methyl-Hg] for seven days in this resulted an inflammatory pro-oxidant response mainly driven macrophages. This led impairment barrier, defective tight-junctions, increased permeability mucus hypersecretion. In addition, wound-healing capacity monolayer...
Chronic exposure to inorganic arsenic [As(III) and As(V)] affects about 200 million people, is linked a greater incidence of certain types cancer. Drinking water the main route exposure, so, in endemic areas, intestinal mucosa constantly exposed metalloid. However, studies on toxicity As are scarce. The objective this study was evaluate chronic As(III) its associated microbiota. For purpose, BALB/c mice were during 6 months through drinking (15 30 mg/L). Treatment with increased reactive...
The chromosomal ccpA gene from Lactobacillus casei ATCC 393 has been cloned and sequenced. It encodes the CcpA protein, a central catabolite regulator belonging to LacI-GalR family of bacterial repressors, shows 54% identity with proteins Bacillus subtilis megaterium. L. was able complement B. mutant. An mutant showed increased doubling times relief repression some enzymatic activities, such as N-acetylglucosaminidase phospho-beta-galactosidase. Detailed analysis activity performed by using...