A5034078205- Glycosylation and Glycoproteins Research
- Advanced Cellulose Research Studies
- Lipid Membrane Structure and Behavior
- Proteoglycans and glycosaminoglycans research
- Carbohydrate Chemistry and Synthesis
- Protein Structure and Dynamics
- NMR spectroscopy and applications
- Advanced NMR Techniques and Applications
- Molecular spectroscopy and chirality
- Monoclonal and Polyclonal Antibodies Research
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Polysaccharides and Plant Cell Walls
- Polysaccharides Composition and Applications
- Protein purification and stability
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Toxin Mechanisms and Immunotoxins
- Fibroblast Growth Factor Research
- Enzyme Production and Characterization
- Surfactants and Colloidal Systems
- Calpain Protease Function and Regulation
- Surface Modification and Superhydrophobicity
- Lignin and Wood Chemistry
- Power Transformer Diagnostics and Insulation
Centro de Investigaciones Científicas Isla de la Cartuja
2012-2025
Instituto de Investigaciones Químicas
2012-2025
Universidad de Sevilla
2015-2025
University of East Anglia
2018-2024
Norwich Research Park
2018-2024
Consejo Superior de Investigaciones Científicas
2013-2024
University of Oxford
2016-2020
Madrid Health Service
2020
Norwich University
2018
Oxfam
2016
G protein-coupled receptors (GPCRs) are the largest class of membrane receptors, playing a key role in regulation processes as varied neurotransmission and immune response. Evidence for GPCR oligomerisation has been accumulating that challenges idea GPCRs function solely monomeric receptors; however, remains controversial primarily due to difficulties comparing evidence from very different types structural dynamic data. Using combination single-molecule ensemble FRET, double...
Langerin is a C-type lectin present on Langerhans cells that mediates capture of pathogens in carbohydrate-dependent manner, leading to subsequent internalization and elimination the cellular organelles called Birbeck granules. This mechanism mediated by langerin was shown constitute natural barrier for HIV-1 particle transmission. Besides interacting specifically with high mannose fucosylated neutral carbohydrate structures, has ability bind sulfated ligands as 6-sulfated galactosides...
Hydrogels have a complex, heterogeneous structure and organisation, making them promising candidates for advanced structural cosmetics applications. Starch is an attractive material producing hydrogels due to its low cost biocompatibility, but the dynamics of polymer chains within starch are not well understood, limiting their development utilisation. We employed range NMR methodologies (CPSP/MAS, HR-MAS, HPDEC WPT-CP) probe molecular mobility water featuring wide physical properties. The...
Surface hydrophobization of cellulose nanomaterials has been used in the development nanofiller-reinforced polymer composites and formulations based on Pickering emulsions. Despite well-known effect hydrophobic domains self-assembly or association water-soluble amphiphiles, very few studies have addressed behavior hydrophobized aqueous media. In this study, we investigate properties nanocrystals (CNCs) their amphiphilic suspensions gels. CNCs different hydrophobicity were synthesized from...
A novel mechanism of monolayer poration leading to the rapid exfoliation and lysis microbial membranes is reported.
Utilization of reversible non-covalent interactions is a versatile design strategy for the development stimuli responsive soft materials. In this study, hydrophobic were harnessed to assemble water-soluble macromolecules and nanoparticles into transient hybrid network forming thermosensitive hydrogels with tunable rheological properties. Hybrid built biopolymer derived components: cellulose nanocrystals (CNCs), high aspect ratio, hydroxypropyl methylcellulose (HPMC). To enable polymer/CNC...
Abstract Based on the structure of regular heparin, we have prepared a smart library heparin‐like trisaccharides by incorporating some sulfate groups in sequence α‐ D ‐GlcNS‐ (1‐4)‐α‐ L ‐Ido2S‐(1‐4)‐α‐ ‐GlcN. According to 3D which features one helix turn every four residues, this fragment corresponds minimum binding motif. We performed complete NMR study and found that similar heparin itself, but their spectral properties are such allow extract very detailed information about distances...
Cellulose nanocrystals (CNCs) that bind to each other through associative hydrophobic interactions have been synthesized by modifying sulfated CNCs (sCNCs) with moieties. These octyl-CNCs form gels at significantly lower concentrations than parent sCNCs, producing extremely strong hydrogels. Unlike these do not ordered liquid crystalline phases indicating a random association into robust network driven interactions. Furthermore, involvement of the multicomponent supramolecular assembly was...
A novel mechanism of heat-triggered gelation for oxidised cellulose nanofibrils (OCNF) is reported. We demonstrate that a synergistic approach combining rheology, small-angle X-ray scattering (SAXS) and saturation transfer difference NMR (STD NMR) experiments enables detailed characterisation at different length scales. OCNF dispersions experience an increase in solid-like behaviour upon heating as evidenced by rheological studies, associated with enhanced interfibrillar interactions...
The use of potential biostimulants is broad interest in plant science for improving yields. application a humic derivative called fulvic acid (FA) may improve forage crop production. FA an uncharacterized mixture chemicals and, although it has been reported to increase growth parameters many species including legumes, its mode action remains unclear. Previous studies the have lacked appropriate controls, and few included field trials. Here we report yield increases due three European...
Helix 8 of the GPCR neurotensin receptor 1 is stabilised by specific lipid-protein interactions.
STD NMR spectroscopy is a powerful ligand-observed tool for screening and characterizing the interactions of small molecules low molecular weight fragments with given macromolecule, identifying main intermolecular contacts in bound state. It also analytical technique accurate determination protein–ligand dissociation constants (KD) medium-to-weak affinity, interest pharmaceutical industry. However, KD epitope mapping requires long series experiments at increasing saturation times to carry...
Low-affinity protein-ligand interactions are important for many biological processes, including cell communication, signal transduction, and immune responses. Structural characterization of these complexes is also critical the development new drugs through fragment-based drug discovery (FBDD), but it challenging due to low affinity fragments binding site. Saturation transfer difference (STD) NMR spectroscopy has revolutionized study low-affinity receptor-ligand enabling detection structural...
Understanding the fine details of self-assembly building blocks into complex hierarchical structures represents a major challenge en route to design and preparation soft-matter materials with specific properties. Enzymatically synthesised cellodextrins are known have limited water solubility beyond DP9, point at which they self-assemble particles resembling antiparallel cellulose II crystalline packing. We prepared characterised series site-selectively fluorinated different degrees...
Enteropathogenic arginine-glycosyltransferases (Arg-GTs) alter higher eukaryotic proteins by attaching a GlcNAc residue to arginine acceptor sites, disrupting essential pathways such as NF-κB signaling, which promotes bacterial survival. These enzymes are potential drug targets for treating related diseases. In this study, we present novel STD NMR Epitope Perturbation Mutation spectroscopic approach that, in combination with hydrogen–deuterium exchange mass spectrometry (HDX-MS), and...
Rotaviruses (RVs) are the main cause of viral diarrhea among infants, small children, and young many animal species. Histo-blood group antigens (HBGAs) potential RV receptors glycan composition on mucous surfaces influences host susceptibility cross-species virus transmission. RVs exhibit genotype-dependent binding differences due to sequence modifications in VP8* domain spike protein VP4. Nevertheless, molecular bases for this specificity, especially non-A RVs, not thoroughly understood....
The polyconformational behavior of l-iduronic acid (L-IdoA2S) in heparin derivatives has been previously analyzed terms intra-ring proton–proton vicinal coupling constants (3JHH) through mathematical fit experimental and theoretical values (Ferro DR, Provasoli A, Ragazzi M, Casu B, Torri G, Bossennec V, Perly Sinay P, Petitou Choay J. 1990. Conformer Populations l-Iduronic Acid Residues Glycosaminoglycan Sequences. Carbohydr Res. 195:157–167; Muñoz-García JC, López-Prados J, Angulo...
Heparin-like saccharides play an essential role in binding to the fibroblast growth factor (FGF)-1 and their membrane receptors receptor forming a ternary complex that is responsible of internalization signal, via dimerization intracellular regions receptor. In this study, we report affinities between five synthetic hexasaccharides with human FGF-1 obtained by surface plasmon resonance experiments, compare induced mitogenic activity previously obtained. These oligosaccharides differ...
The motional behaviour of heparin oligosaccharides in solution is best described as a top rotor having two perpendicular rotation axes. This prevents an accurate extraction interprotonic distances by NOESY/ROESY based methods. In this paper, we describe the structure hexasaccharide 1 calculated from high exactitude distance data obtained off-resonance ROESY combined with long MD simulation 500 ns. previous studies, have found that synthetic hexasaccharides sulphate groups directed towards...
Aggregation in aqueous dispersions of zwitterionic cellulose nanofibrils can be controlled by addition cationic and anionic surfactants.
The sensitive, rapid and accurate diagnosis of