A5076169110- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Hepatitis B Virus Studies
- Antibiotics Pharmacokinetics and Efficacy
- SARS-CoV-2 and COVID-19 Research
- Vibrio bacteria research studies
- Identification and Quantification in Food
- SARS-CoV-2 detection and testing
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
- Hepatitis C virus research
- Antimicrobial Resistance in Staphylococcus
- Mycobacterium research and diagnosis
- Respiratory viral infections research
- Microbial infections and disease research
- Bacterial biofilms and quorum sensing
- Salmonella and Campylobacter epidemiology
- COVID-19 epidemiological studies
- Aquaculture disease management and microbiota
- Liver Disease Diagnosis and Treatment
- Species Distribution and Climate Change
- Hepatitis Viruses Studies and Epidemiology
- Antibiotic Use and Resistance
- COVID-19 diagnosis using AI
Institute for Integrative Systems Biology
2017-2025
Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana
2016-2025
Universitat de València
2016-2024
Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública
2017-2018
Clinica Universidad de Navarra
2004
Bacteriophages play key roles in bacterial ecology and evolution are potential antimicrobials. However, the determinants of phage-host specificity remain elusive. Here, we isolate 46 phages to challenge 138 representative clinical isolates Klebsiella pneumoniae, a widespread opportunistic pathogen. Spot tests show narrow host range for most phages, with <2% 6,319 combinations tested yielding detectable interactions. Bacterial capsule diversity is main factor restricting phage range....
Mapping of high-throughput sequencing (HTS) reads to a single arbitrary reference genome is frequently used approach in microbial genomics. However, the choice may represent source errors that affect subsequent analyses such as detection nucleotide polymorphisms (SNPs) and phylogenetic inference. In this work, we evaluated effect on short-read sequence data from five clinically epidemiologically relevant bacteria ( Klebsiella pneumoniae , Legionella pneumophila Neisseria gonorrhoeae...
Vibrio vulnificus is a pathogen of public health concern that causes either primary septicemia after ingestion raw shellfish or secondary wound exposure to seawater. In consequence, and seawater are considered its main reservoirs. However, there one aspect biology systematically overlooked: association with fish in natural environment. This led 1975 the emergence zoonotic clade within phylogenetic lineage 2 following successive outbreaks vibriosis farmed eels. Although this now worldwide...
Carbapenem-resistant Klebsiella pneumoniae is a major cause of hospital-acquired infections and the fastest-growing pathogen in Europe. Carbapenem resistance was detected at Consorcio Hospital General Universitario de Valencia (CHGUV) early 2015, there has been significant increase carbapenem-resistant isolates since then. In this study, we collected from hospital during period (from 2015 to 2019) studied how K. emerged spread hospital. A total 225 were subjected whole-genome sequencing with...
Since the discovery of blaNDM-1, NDM β-lactamases have become one most widespread carbapenemases worldwide. To date, 43 different variants been reported but some, such as blaNDM-23, not characterized in detail yet. Here, we describe emergence a novel blaNDM-23 allele from blaNDM-1 ancestor and multidrug resistance plasmid that has disseminated it through Klebsiella pneumoniae ST437 clone several Spanish hospitals. Between 2016 2019, 1,972 isolates were collected an epidemiological survey for...
Abstract Background Klebsiella pneumoniae is a highly prevalent antimicrobial-resistant (AMR) pathogen in healthcare-settings worldwide, especially third-generation cephalosporin (3GC) and carbapenem-resistant strains. In the Comunitat Valenciana, Spain, these strains are growing concern due to their increasing prevalence limited treatment options. Fortunately, Valenciana has well-established microbiological surveillance system, called RedMIVA, that gathers all tests performed at...
Summary The emergence of resistance‐associated substitutions (RASs) can compromise the high efficacy direct‐acting antivirals (DAAs). Little is known about RASs selection at very early time points during DAA treatment. Therefore, we analyzed potential immediately after therapy initiation. Samples 71 patients treated with different DAAs were collected baseline, (hours 4 and 8; days 1‐7; weeks 2‐4) or until target not detected. HCV‐RNA levels determined by qPCR, detected deep sequencing....
The aim of this study has been to characterize carbapenem-resistant Klebsiella pneumoniae isolates and determine the resistance mechanisms involved, clonal relationship between strains clinical demographical data infected patients.Clinical from patients were collected statistically analysed. Antimicrobial susceptibility testing was performed genes detected both phenotypically genotypically. Conjugation assays show horizontal transferability genes. Clonal also studied. Next-generation...
Potentially zoonotic Vibrio vulnificus strains were isolated from vibriosis outbreaks occurring on eastern Mediterranean tilapia farms between 2016 and 2019. In this work, the draft genome sequences of three representative isolates are presented.
Illness due to the hepatitis B virus is an enormous problem for international public health, affecting over 300 million persons throughout world, although its prevalence varies considerably between different geographic areas. The universal vaccination of newly born, promulgated by World Health Organisation, has made it possible partially control spread in many countries, including Spain. However, vaccine does not generate protective antibodies approximately 5% population. Besides, infection...
ABSTRACT Bacteriophages play key roles in bacterial ecology and evolution are potential antimicrobials. However, the determinants of phage-host specificity remain elusive. Here, we used 46 newly-isolated phages to challenge 138 representative clinical isolates Klebsiella pneumoniae , a widespread opportunistic pathogen. Spot tests revealed narrow host range for most phages, with <2% 6319 combinations tested yielding detectable interactions. Bacterial capsule diversity was main factor...
The first descriptions of reinfection by SARS-CoV-2 have been recently reported. However, these studies focus exclusively on the reinfected case, without considering epidemiological context event. Our objectives were to perform a complete analysis sequential infections and community transmission events around reinfection, including infection preceding it, exposure, subsequent transmissions. was supported host genetics, viral whole-genome sequencing, phylogenomic population analysis, refined...
ABSTRACT The COVID-19 pandemic has shaken the world since beginning of 2020. Spain is among European countries with highest incidence disease during first wave. We established a multidisciplinar consortium to monitor and study evolution epidemic, aim contributing decision making stopping rapid spreading across country. present results for 2170 sequences from wave SARS-Cov-2 epidemic in representing 12% diagnosed cases until 14 th March. This effort allows us document at least 500 initial...
Abstract Klebsiella pneumoniae is an important cause of healthcare-associated infections, with high levels antimicrobial resistance (AMR) to critical antibiotics such as carbapenems and third-generation cephalosporins (3GCs). Accurate susceptibility detection essential for guiding appropriate treatment. In this study, we evaluated the efficacy machine learning (ML) models predicting AMR phenotypes in K. particularly which rule-based approaches fail. We analyzed a dataset 5,907 genomes from...