Mapping of high-throughput sequencing (HTS) reads to a single arbitrary reference genome is frequently used approach in microbial genomics. However, the choice may represent source errors that affect subsequent analyses such as detection nucleotide polymorphisms (SNPs) and phylogenetic inference. In this work, we evaluated effect on short-read sequence data from five clinically epidemiologically relevant bacteria ( Klebsiella pneumoniae , Legionella pneumophila Neisseria gonorrhoeae...

Salmonella enterica is one of the most important foodborne pathogens around world. In last years, S. serovar Infantis has become an emerging pathogen in many countries, often as multidrug resistant clones. To understand importance broiler industry Ecuador, we performed a study based on phenotypic and WGS data isolates from poultry farms, chicken carcasses humans. We showed high prevalence farms (41.4%) (55.5%), but low (1.98%) human samples. was shown to be prevalent with 98.2%, 97.8% 50%...

Abstract One major health issue is the microbial and chemical contamination of natural freshwater, particularly in Latin American countries, such as Ecuador, where it still lacking wastewater treatment plants. This study analyzed water quality twelve rivers Ecuador (Coastal, Andean, Amazonian regions). All showed levels E. coli total coliforms above maximum limit according to International Ecuadorian legislations. The most polluted were Zamora, Esmeraldas Machángara. Also, pathotypes found...

Human Papillomavirus (HPV) is the most common sexually transmitted infection worldwide and it responsible for cases of uterine cancer. In Ecuador there limited information about HPV types (and variants) in cancerous lesions; however, identifying type‐specific prevalence cervical lesions women living important to better predict impact prophylactic vaccination this country. We studied or precancerous from 164 Ecuadorian found that 86.0% were positive. The HPV16 (41.8%) HPV58 (30.5%)....

The increase in the resistance of bacteria to antibiotics represents a priority problem be addressed for humanity because it causes infectious diseases become increasingly difficult or impossible treat, which could lead reduction hope and quality life. For this reason, medicinal plants have great alternative treatment infections reducing bacterial resistance, through search new compounds present various plant species. Kalanchoe daigremontiana is species from Crassulaceae family, native...

Indigenous beers ( chicha ) are part of the indigenous culture in Ecuador. The fermentation process these probably relies on microorganisms from fermented substrates, environment and human microbiota. We analyzed microbiota artisanal (including a type beer produced after chewing boiled cassava) using bacterial 16S ribosomal RNA (rRNA) gene-based tag-encoded FLX amplicon pyrosequencing (bTEFAP). Surprisingly, we found that Streptococcus salivarius mutans (part oral microbiota) were among most...

Abstract Background Cutaneous leishmaniasis is endemic to the Pacific coast of Ecuador, and Nyssomyia trapidoi considered be its main vector. Dujardin et al. [1] recorded some differences in body pigmentation isoenzymatic profiles sympatric populations Ny. from Ecuador suggested existence two cryptic species. Methods Entomological collections were performed November 2008 March 2011 locality Paraíso Escondido using CDC miniature light traps human bait. Morphological, isoenzymatical molecular...

Salmonella enterica is among the most important foodborne pathogens. In Ecuador, there limited information about non-typhoidal S. occurrence in raw meats, its serotype distribution, and antimicrobial resistance. this study, we addressed issue 1095 retail fresh meats (chicken, pork, veal, lamb, beef, turkey) Quito by performing a traditional culture methodology molecular detection. We found that was present 38.1% of samples, Infantis common showing high antibiotic resistance wide host range....

In Ecuador, cervical cancer is the third most common among women and second cause of cancer-related death in women. Although HPV represents a serious public health problem worldwide, information about its prevalence genotypes remote communities Ecuador limited. The aim this study was to determine distribution ethnic minority from two northwestern region Ecuador: Afro-Ecuadorians Chachis (Amerindian group).

Leptospirosis is the most common zoonosis worldwide, and increasingly in poor urban communities, where there inadequate sewage disposal abundance of domestic peridomestic animals. There are many risk factors associated with disease, such as contaminated water exposure, close contact animals, floods, recreational activities related to water, wet agriculture. The symptoms leptospirosis other infectious diseases and, if not treated, it can lead meningitis, liver failure, kidney damage death....

Listeria monocytogenes is a foodborne pathogen that can produce serious, even fatal, infections. Among other foods, it be found in unpasteurized dairy and ready-to-eat products. Surveillance of L. great interest since sources infection are difficult to determine due the long incubation period, because symptoms listeriosis similar diseases. We performed genomic study isolated from fresh cheeses clinical samples Ecuador. Sixty-five isolates were evaluated sequenced, 14 cheese 20 cases National...

Listeria monocytogenes is one of the most important bacteria associated with foodborne diseases, soft cheese being an L. vehicle. In Ecuador, consumed in 84.3% urban households. We determined prevalence and serogroups 260 fresh artisanal samples collected 18 24 Ecuadorian provinces. spp. detection was carried out by culture-dependent independent methods; 14.23% were positive for monocytogenes. Serogroup IVb found 83.78% food isolates. Serogroups IIb IIa present 8.11% our To knowledge, this...

La soya es un cultivo cuya producción nacional en el Ecuador no suficiente para suplir la demanda interna. Por esta razón, se recurre a importación de semilla siembra y grano consumo, desde países donde uso transgénica ha sido aprobado. Como consecuencia, existe probabilidad del ingreso este territorio ecuatoriano. En investigación, estandarizó una metodología detección cuantificación genéticamente modificada con tecnología SYBR Green. Los primers utilizados amplificación corresponden...

Indigenous beers ( chicha ) are part of the indigenous culture in Ecuador. The fermentation process these relay probably on microorganisms from: fermenting substrates, environment and human microbiota. We analyzed microbiota artisanal (including a type beer produced after chewing boiled cassava) using bacterial 16S-based tag-encoded FLX amplicon pyrosequencing (bTEFAP). Surprisingly, we found that Streptococcus salivarius S treptococcus mutans (part oral microbiota) where among most abundant...

Background Leptospirosis is one of the most common zoonoses in world which associated with a severe febrile disease humans causing variety syndromes including meningitis, interstitial nephritis, hepatitis, and sometimes death. caused by different pathogenic Leptospira species divided into almost 30 serogroups more than 300 serovars are carried some animal asymptomatic chronic infections. Humans can become infected through direct contact urine or indirectly coming fresh water mud contaminated...

Cervical cancer is the fourth most common type of in women. Worldwide, it a public health problem with around 604,127 women diagnosed per year and 341,831 deaths. persistent high-risk human papillomavirus (HPV) infection are highly associated. However, other factors also involved, such as viral load, HPV variants, sexual behavior, genetic factors. The host immune response against has been widely studied shown associations development cervical cancer. leukocyte antigen (HLA) genes related to...

Abstract Mapping of high-throughput sequencing (HTS) reads to a single arbitrary reference genome is frequently used approach in microbial genomics. However, the choice may represent source errors that affect subsequent analyses such as detection nucleotide polymorphisms (SNPs) and phylogenetic inference. In this work, we evaluated effect on short-read sequence data from five clinically epidemiologically relevant bacteria ( Klebsiella pneumoniae , Legionella pneumophila Neisseria gonorrhoeae...

Indigenous beers ( chicha ) are part of the indigenous culture in Ecuador. The fermentation process these relay probably on microorganisms from: fermenting substrates, environment and human microbiota. We analyzed microbiota artisanal (including a type beer produced after chewing boiled cassava) using bacterial 16S-based tag-encoded FLX amplicon pyrosequencing (bTEFAP). Surprisingly, we found that Streptococcus salivarius S treptococcus mutans (part oral microbiota) where among most abundant...

Salmonella enterica subspecies is one of the most important foodborne pathogens and causative agent salmonellosis, which affects both humans animals producing numerous infections every year. The study understanding its epidemiology are key to monitoring controlling these bacteria. With development whole-genome sequencing (WGS) technologies, surveillance based on traditional serotyping phenotypic tests resistance being replaced by genomic surveillance. To introduce WGS as a routine...