A5059539794- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Identification and Quantification in Food
- Aquaculture Nutrition and Growth
- Salmonella and Campylobacter epidemiology
- Yersinia bacterium, plague, ectoparasites research
- Invertebrate Immune Response Mechanisms
- Microbial infections and disease research
- Bacterial biofilms and quorum sensing
- Hemoglobin structure and function
- Viral Infections and Vectors
- Escherichia coli research studies
- Erythrocyte Function and Pathophysiology
- Immune Response and Inflammation
- Bacterial Identification and Susceptibility Testing
- Plant Pathogenic Bacteria Studies
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Iron Metabolism and Disorders
- Fish Biology and Ecology Studies
- Antibiotic Use and Resistance
- Gut microbiota and health
- Service-Learning and Community Engagement
- HIV Research and Treatment
- Environmental Chemistry and Analysis
Universitat de València
2015-2025
Instituto de Biomedicina de Valencia
2018-2025
Universidade de Santiago de Compostela
1989-2000
Universidad de Santiago de Chile
1993
Royal Agricultural University
1992
The constant increase of aquaculture production and wealthy seafood consumption has forced the industry to explore alternative more sustainable raw aquafeed materials, plant ingredients have been used replace marine feedstuffs in many farmed fish. objective present study was assess whether plant-based diets can induce changes intestinal mucus proteome, gut autochthonous microbiota disease susceptibility fish, these could be reversed by addition sodium butyrate diets. Three different trials...
Abstract Tilapia culture is an important source of income and nutrition to many rural families. Since 2000, the production tilapia increased reached domestic global markets. Major farmed species Nile ( Oreochromis niloticus ), in earthen ponds cage cultures. Intensification contributed disease outbreaks, with bacterial infections causing mortalities morbidities, threatening sustainable production. At farms, high nutrient concentrations, water temperature fish densities enhance growth...
The routine use of chemotherapy to control bacterial diseases in aquatic populations has resulted the development and spread antibiotic resistance. inclusion immunostimulants fish diets (functional diets) is one main strategies solve this threat. This study aimed analyse intestinal microbiota cultured European sea bass (Dicentrarchus labrax) fed two functional applying pyrosequencing PCR-amplified 16S rRNA gene. Quality-filtered reads were assigned family genus taxonomic levels using...
Vibrio vulnificus biotype 2 is classically considered an obligate eel pathogen. However, it has recently been associated with one human septicemic case. In this paper, the opportunistic behavior of pathogen discussed. The bacterium can survive alone in brackish water or attached to surfaces for at least 14 days. It able spread through and infect healthy eels by using skin as a portal entry. These results suggest that infected may act reservoirs infection. A capsule seems be essential...
We compared Pasteurella piscicida strains isolated from different fish species in several European countries with Japan and the United States. The taxonomic analysis revealed that, regardless of geographic origin source isolation, all exhibited same biochemical physiological characteristics. Serological assays rabbit antisera demonstrated a high level antigenic similarity among strains, cross-agglutination titers 20,480 to 40,960. This serological homogeneity was supported by...
During a 3 yr penod several epizootics of vibnos~s In turbot Scophthalmus maximus occurred in different marine farms located northwestern Spain.Affected fish showed extensive haemorragic areas around the anus, eyes and mouth, as well characteristic accumulation mucus reddish fluid peritonea1 cavity.Strains Vibrio damsela were isolated from diseased fish, representing, to our knowledge, first report this bacterium causing problems cultured turbot.Isolates biochemically identical collection...
Abstract Yersinia ruckeri , the causal agent of enteric redmouth (ERM) disease, was isolated from epizootics that occurred in different Spanish rainbow trout, Oncorhynchus mykiss (Walbaum), farms which vaccination against ERM had been performed. In all episodes, most pronounced clinical signs exhibited by affected fish were severe haemorrhages mouth, eyes and around vent. The isolates identified as Y. serovar I 16S rRNA sequencing together with serological tests. They lacked motility lipase...
Infectious diseases and fish feeds management are probably the major expenses in aquaculture business. Hence, it is a priority to define sustainable strategies which simultaneously avoid therapeutic procedures reinforce immunity. Currently, one preferred approach use of immunostimulants can be supplemented diets. Arginine versatile amino acid with important mechanisms closely related immune response. Aiming at finding out how arginine affects innate status or improve disease resistance...
ABSTRACT Vibrio vulnificus biotype 2 is the etiological agent of warm-water vibriosis, a disease that affects eels and other teleosts, especially in fish farms. Biotype polyphyletic probably emerged from aquatic bacteria by acquisition transferable virulence plasmid encodes resistance to innate immunity teleosts. Interestingly, comprises zoonotic clonal complex designated as serovar E has extended worldwide. One most interesting factors produced RtxA1 3 , multifunctional protein acts lethal...
Vibrio vulnificus (Vv) is a multi-host pathogenic species currently subdivided into three biotypes (Bts). The Bts are human-pathogens, but only Bt2 also fish-pathogen, an ability that conferred by transferable virulence-plasmid (pVvbt2). Here we present phylogenomic analysis from the core genome of 80 Vv strains belonging to recovered wide range geographical and ecological sources. We have identified five well-supported phylogenetic groups or lineages (L). L1 comprises mixture clinical...
Journal Article Evidence that water transmits the disease caused by fish pathogen Photobacterium damselae subsp. Get access B. Fouz, Fouz Departamento de Microbiología y Parasitología,Universidad Santiago Compostela, andDepartamento Microbiología, Universidad Valencia, Burjasot, Spain Search for other works this author on: Oxford Academic Google Scholar A. E. Toranzo, Toranzo and M. Milán, Milán C. Amaro of Applied Microbiology, Volume 88, Issue 3, 1 March 2000, Pages 531–535,...
Strains of Vibrio vuln~flcus biotype 2 were recovered from diseased European eels Angujlla anguilla during epizootics These stralns biochemically and serologically identical to the ATCC 33149 strain, which was lsolated Japanese eel japonica.The isolates more virulent for elvers (LD50 < 10' cfu) than strain used as control (LDS0 = 104 cfu).All strains able adhere human fish cell lines also strongly cytotoxic.The extracellu1a1-products (ECPs) exhibited lipolytic, phospholipolytic, proteolytic,...
The virulence mechanisms of Vibrio vulnificus biotype 2 have been studied and compared with those 1 in mice as the experimental animals. Biotype isolates from European eels were virulent for strains (50% lethal dose, about 10(5) CFU per mouse); a septicemic infection developed less than 24 h. These had several properties common organisms including capsule expression, uptake various iron sources, production exoproteins, whose role mouse has demonstrated. We also discuss implication human infections.
ABSTRACT Vibrio vulnificus is a heterogeneous bacterial species that comprises virulent and avirulent strains from environmental clinical sources have been grouped into three biotypes. To validate the typing methods proposed to distinguish isolates, we performed phenotypic (API 20E, API 20NE, BIOLOG tests) genetic (ribotyping DNA polymorphism at several loci) studies with large strain collection representing different biotypes, origins, host ranges. No method was useful for biotyping or...
Vibrio vulnificus is a zoonotic pathogen that lives in temperate, tropical and subtropical aquatic ecosystems whose geographical distribution expanding due to global warming. The species genetically variable only the strains belong clonal-complex can cause vibriosis both humans fish (being its main host eel). Interestingly, severity of eel human depends largely on water temperature (highly virulent at 28°C, avirulent 20°C or below) iron content blood, respectively. objective this work was...
ABSTRACT Vibrio vulnificus serovar E (formerly biotype 2) is the etiologic agent that responsible for main infectious disease affecting farmed eels. Although pathogen can theoretically use water as a vehicle transmission, it has not been isolated from tank during epizootics to date. In this work, mode of transmission healthy eels, portals entry into fish, and their putative reservoirs have investigated by means laboratory field experiments. Results experiments direct indirect host-to-host...
Vibriosis due to Vibrio vulnificus serovar E (biotype 2) is one of the main causes mortality in European eels cultured Europe. The objective this study was develop a vaccine and vaccination procedure against pathogen. With aim, we tested several formulations (inactivated whole-cells with without toxoids--inactivated extracellular products--from capsulated uncapsulated strains, attenuated live vaccines purified lipopolysaccharide [LPS]) on maintained under controlled laboratory conditions...
A new disease occurred from August to October 1987 in cultured turbot northwestern Spain. During the outbreak, there was continuous but low mortality, with a total cumulative loss of 4% affected population. Diseased did not display unusual swimming behavior; external signs were abdominal distension and hemorrhagic foci anus base fins. Internal examination fish revealed swollen stomach intestine, which filled mucous liquid. The internal wall peritoneal cavity hemorrhagic, an accumulation...
Vibrio vulnificus is a zoonotic pathogen linked to aquaculture that spreading due climate change. The can be transmitted humans and animals by ingestion of raw shellfish or seafood feed, respectively. aim this work was design test new procedure detect V. hazardous human and/or animal health in food/feed samples. For purpose, we combined pre-enrichment step with multiplex PCR using primers for the species virulence markers. In vitro assays mixed DNA from different cultures showed protocol 100...
Acute hepatopancreatic necrosis (AHPND) is an emerging severe disease caused by strains of Vibrio parahaemolyticus (VpAHPND) in whiteleg shrimp (Litopenaeus vannamei). Mitigating its negative impact, and at the same time minimizing antibiotics treatments, major challenge aquaculture. A sustainable strategy could be to include immunostimulants diet. Phytobiotics, harmless plant extracts with immunostimulatory biocidal activities, are promising candidates. In this study, we evaluated...
Vibrio vulnificus is a gram-negative bacterium capable of producing septicemic infections in eels and immunocompromised humans. Two biotypes are classically recognized, with the virulence for being specific to strains belonging biotype 2, which constitutes homogeneous lipopolysaccharide (LPS)-based O serogroup (which we have designated E). In present study demonstrated that side chain this LPS determines selective 2 eels: (i) 1 do not belong E) destroyed by bactericidal action nonimmune eel...