A5002424657- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- French Urban and Social Studies
- Pneumonia and Respiratory Infections
- Bacterial Genetics and Biotechnology
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Intensive Care Unit Cognitive Disorders
- Cystic Fibrosis Research Advances
- Fermentation and Sensory Analysis
- Fungal and yeast genetics research
- Respiratory viral infections research
- RNA and protein synthesis mechanisms
- SARS-CoV-2 and COVID-19 Research
- Inhalation and Respiratory Drug Delivery
- Social Sciences and Governance
- Microbial infections and disease research
- Biochemical and Structural Characterization
- Microbial Metabolism and Applications
- Mechanical Circulatory Support Devices
- Bacterial Infections and Vaccines
- Bacterial Identification and Susceptibility Testing
- Neonatal and Maternal Infections
Debiopharm (Switzerland)
2023-2025
University Hospital of Bern
2019-2025
University of Bern
2019-2025
University of Dundee
2021
Medical Research Council
2021
Monash University
2011-2020
Australian Regenerative Medicine Institute
2015-2020
Northeastern University
2018-2020
UK Centre for Ecology & Hydrology
2019
Wilfrid Laurier University
2017
Background Daptomycin remains one of our last-line anti-staphylococcal agents. This study aims to characterize the genetic evolution daptomycin resistance in S. aureus. Methods Whole genome sequencing was performed on a unique collection isogenic, clinical (21 strains) and laboratory (12 derived strains that had been exposed developed daptomycin-nonsusceptibility. Electron microscopy (EM) lipid membrane studies were selected isolates. Results On average, six coding region mutations observed...
Abstract In contrast to the many reports of successful real-world cases personalized bacteriophage therapy (BT), randomized controlled trials non-personalized products have not produced expected results. Here we present outcomes a retrospective observational analysis first 100 consecutive BT difficult-to-treat infections facilitated by Belgian consortium in 35 hospitals, 29 cities and 12 countries during period from 1 January 2008 30 April 2022. We assessed how often positive clinical...
The mannoprotein which is a major component of the cell wall Saccharomyces cerevisiae an effective bioemulsifier. Mannoprotein emulsifier was extracted in high yield from whole cells fresh bakers' yeast by two methods, autoclaving neutral citrate buffer and digestion with Zymolase (Miles Laboratories; Toronto, Ontario, Canada), beta-1,3-glucanase. Heat-extracted purified ultrafiltration contained approximately 44% carbohydrate (mannose) 17% protein. Treatment protease eliminated...
Significance Staphylococcus aureus is one of the most significant human bacterial pathogens that has capacity to cause serious infections and become highly resistant antibiotics. In this study, we identified a metabolic adaptation mechanism used by S. simultaneously circumvent killing last-line antistaphylococcal antibiotics, daptomycin, attack from host innate immune cells. This process led enhanced survival was mediated change in membrane phospholipid composition sufficient impair...
The fusion-generating phage lambda plac Mu1 was used to produce fusions of lacZ fhuA, the gene encoding ferrichrome-iron receptor (FhuA protein) in outer membrane Escherichia coli K-12. Fusions fhuA a delta (lac) strain were selected by their resistance bacteriophage phi 80 vir. Ten independent (fhuA'-'lacZ) all Lac+ and resistant lethal agents which require FhuA protein as receptor, i.e., vir, T5, T1, UC-1, colicin M; none could utilize ferrichrome sole iron source. Specialized transducing...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetermination of interfacial areas in emulsions using turbidimetric and droplet size data: correction the formula for emulsifying activity indexDavid R. Cameron, Martin E. Weber, Edmund S. Idziak, Ron J. Neufeld, David G. CooperCite this: Agric. Food Chem. 1991, 39, 4, 655–659Publication Date (Print):April 1, 1991Publication History Published online1 May 2002Published inissue 1 April...
The genetic mechanisms that contribute to reduced susceptibility vancomycin in Staphylococcus aureus are complex and heterogeneous. In addition, debate is emerging as the true effect of on staphylococcal virulence. To investigate this, comparative genomics was performed a collection vancomycin-exposed isogenic S. pairs (14 strains total). Previously described mutations were observed genes such vraG, agrA, yvqF, rpoB; however, new mechanism identified involving serine/threonine phosphatase,...
The occurrence of mutations in methicillin-resistant Staphylococcus aureus (MRSA) during persistent infection leads to antimicrobial resistance but may also impact host-pathogen interactions. Here, we investigate the consequences 2 arising clinical MRSA infection: RpoB H481Y, which is linked rifampicin resistance, and RelA F128Y, associated with an active stringent response. Allelic exchange experiments showed that both cause global transcriptional changes, leading upregulation capsule...
Sirtuins are an ancient family of NAD(+)-dependent deacylases connected with the regulation fundamental cellular processes including metabolic homeostasis and genome integrity. We show existence a hitherto unrecognized class sirtuins, found predominantly in microbial pathogens. In contrast to earlier described classes, these sirtuins exhibit robust protein ADP-ribosylation activity. our model organisms, Staphylococcus aureus Streptococcus pyogenes, activity is dependent on prior lipoylation...
Persisters represent a small subpopulation of cells within bacterial culture that are tolerant to killing by antibiotics. have been linked recalcitrant infections caused numerous pathogens, including Pseudomonas aeruginosa A classic example is the incurable infection airways for patients with cystic fibrosis. The genetic mediators persister formation P. poorly understood. We generated high-density transposon insertion library PAO1 and determined relative frequency each following...
Daptomycin is a lipopeptide antibiotic used clinically for the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections. The emergence daptomycin-nonsusceptible S. isolates during therapy often associated with multiple genetic changes; however, relative contributions these changes to resistance and other phenotypic usually remain unclear. present study was undertaken investigate this issue using genetically characterized series four isogenic clinical MRSA strains derived...
Rationale: Infections caused by multidrug-resistant bacteria are a major clinical challenge. Phage therapy is promising alternative antibacterial strategy.Objectives: To evaluate the efficacy of intravenous phage for treatment ventilator-associated pneumonia due to methicillin-resistant Staphylococcus aureus in rats.Methods: In randomized, blinded, controlled experimental study, we compared teicoplanin (3 mg/kg, n = 12), cocktail four phages (2–3 × 109 plaque-forming units/ml 2003, 2002, 3A,...
Despite intensive antibiotic treatment, Pseudomonas aeruginosa often persists in the airways of cystic fibrosis (CF) patients for decades, and can do so without resistance development. Using high-throughput screening assays bacterial survival after treatment with high concentrations ciprofloxacin, we have determined prevalence persisters a large patient cohort using 460 longitudinal isolates P. from 39 CF patients. Isolates were classed as persister variants (Hip) if they regrew following at...
Staphylococcus capitis is an opportunistic pathogen of the coagulase negative staphylococci (CoNS). Functional genomic studies S. have thus far been limited by a lack available complete genome sequences. Here, we determined closed and methylome using Single Molecule Real Time (SMRT) sequencing. The strain, AYP1020, harbors single circular chromosome 2.44 Mb encoding 2304 predicted proteins, which smallest all staphylococcal genomes sequenced to date. AYP1020 two large mobile genetic...
Abstract The treatment of infections caused by methicillin-resistant Staphylococcus aureus is complicated the emergence strains with intermediate-level resistance to vancomycin (termed VISA). We have characterised a molecular pathway involved in vivo evolution VISA mediated regulatory proteins YycH and YycI. In contrast their function other bacterial species, we report positive role for these auxiliary regulation two-component regulator WalRK. Transcriptional profiling yycH yycI deletion...
The optimal method for delivering phages in the context of ventilator-associated pneumonia (VAP) is unknown. In current study, we assessed utility aerosolized (aerophages) experimental methicillin-resistant Staphylococcus aureus (MRSA) pneumonia.Rats were ventilated 4 hours before induction pneumonia. Animals received one following: (1) aerophages; (2) intravenous (IV) phages; (3) a combination IV and (4) linezolid; or (5) linezolid aerophages. Phages administered at 2, 12, 24, 48, 72 hours,...
Abstract A novel biodrying process was investigated whose goal to increase the calorific value of pulp and paper wastewater sludge for efficient combustion in woodwaste furnaces. sludge-woodwaste mixture dried using forced air, where drying rates were enhanced by exothermic metabolic activity aerobic thermophilic microorganisms. Data collected throughout monitor temperature, humidity, pressure, airflow, total mass so that moisture carbon losses could be estimated three sludge/woodwaste...
ABSTRACT An unorthodox, surprising mechanism of resistance to the antibiotic linezolid was revealed by cryo-electron microscopy (cryo-EM) in 70S ribosomes from a clinical isolate Staphylococcus aureus . This high-resolution structural information demonstrated that single amino acid deletion ribosomal protein uL3 confers despite being located 24 Å away binding pocket peptidyl-transferase center. The mutation induces cascade allosteric rearrangements rRNA ultimately results alteration site....